LOCUS       sequence-303176-        3429 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NRCAM-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..3429
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caaatgattt ctgccctgga agtgcctctg gaccccaagc tgctggaaga tctggtgcag
       61 cctcctacca tcacacagca gagccccaag gactacatca tcgaccccag agaaaacatc
      121 gtgatccagt gcgaggccaa gggcaagcct cctccaagct ttagctggac cagaaacggc
      181 acccacttcg atattgacaa ggaccctctg gtcaccatga agcccggaac cggcacactg
      241 atcatcaaca tcatgagcga gggcaaagcc gagacatacg agggcgtgta ccagtgcacc
      301 gccagaaatg aacggggagc cgccgtgtcc aacaacatcg tcgttagacc cagcagatcc
      361 cctctgtgga ccaaagagaa gctggaaccc atcactctgc agtccggcca gtctctggtg
      421 ctgccttgta gacctcctat cggcctgcct cctcctatca tcttctggat ggacaacagc
      481 ttccagcggc tgccccagag cgaaagagtt agccaaggcc tgaacggcga cctgtacttc
      541 agcaacgtgc tgcccgagga caccagagag gattacatct gctacgcccg gttcaatcac
      601 acccagacca tccagcagaa acagcccatc agcgtgaaag tgatcagcgt ggacgagctg
      661 aacgacacaa tcgccgccaa cctgagcgac accgagtttt acggcgccaa gagcagcaga
      721 gagcggcctc caacatttct gacccctgag ggcaacgcca gcaacaaaga ggaactgcgg
      781 ggaaacgtgc tgagcctgga atgtattgcc gagggcctgc ctacaccaat tatctactgg
      841 gccaaagagg acggcatgct gcccaagaac cggaccgtgt acaagaactt cgagaaaacc
      901 ctgcagatca tccacgtgtc cgaggccgac agcggcaact atcagtgtat cgccaagaat
      961 gccctgggcg ccatccacca cacaatcagc gttagagtga aggccgctcc ttattggatc
     1021 acagcccctc agaacctggt gctgtctcct ggcgaagatg gcaccctgat ctgtagagcc
     1081 aacggcaacc ccaagcctcg gatcagctgg ctgacaaatg gcgtgcccat cgagatcgcc
     1141 cctgacgacc ctagcagaaa gatcgacggc gacaccatca tcttttccaa cgtgcaagag
     1201 cggagcagcg ccgtctacca gtgtaatgcc agcaacgagt acggctacct gctggccaac
     1261 gccttcgtga atgtgctggc cgagcctcct agaatcctga caccagccaa cacactgtac
     1321 caagtgatcg ccaacagacc cgctctgctg gactgcgcct tttttggctc tcccctgcct
     1381 accatcgagt ggttcaaagg cgccaaggga tctgccctgc acgaggacat ctatgtgctg
     1441 cacgagaacg gaaccctgga aatccccgtg gctcagaagg atagcaccgg cacctatacc
     1501 tgtgtggcca gaaacaagct cggcatggcc aagaacgaag tgcacctcga gatcaaggac
     1561 cccacctgga tcgtgaagca gcctgagtat gctgtggtgc agaggggctc catggtgtcc
     1621 ttcgagtgca aagtgaagca cgatcacacc ctgagcctga ccgtgctgtg gctgaaggac
     1681 aatagagagc tgcccagcga cgagcggttc accgtggata aggatcatct ggtggtggcc
     1741 gatgtgtccg acgatgactc tggcacctac acatgcgtgg caaacaccac actggacagc
     1801 gtgtcagctt ccgccgtgct gtctgtggtt gcccctacac ctacaccagc tccagtgtac
     1861 gacgtgccca atcctccttt cgacctggaa ctgaccgacc agctggacaa gagcgtgcag
     1921 ctgtcttgga cacccggcga cgacaacaac agccccatca ccaagttcat catcgagtac
     1981 gaggacgcca tgcacaagcc tggactgtgg caccatcaga ccgaagtgtc tggcacccag
     2041 acaactgccc agctgaagct gagcccctac gtgaactact ccttccgcgt gatggccgtg
     2101 aacagcatcg gcaagtctct gccttctgag gccagcgagc agtacctgac aaaggcctcc
     2161 gagcctgaca agaaccctac agccgttgaa ggcctgggca gcgagcctga taatctggtc
     2221 atcacctgga agcccctgaa tggcttcgag agcaatggcc ctggcctgca gtataaggtt
     2281 tcctggcgac agaaggatgg cgacgacgag tggacatctg tggtggtcgc taacgtgtcc
     2341 aagtatatcg tgtccggcac acccaccttc gtgccctacc tgattaaggt gcaggccctg
     2401 aacgatatgg gcttcgctcc tgaacctgcc gtggtcatgg gacacagcgg agaggatctg
     2461 cctatggtgg ctcctggcaa tgtgcgcgtg aacgtggtca atagcacact ggccgaagtg
     2521 cattgggacc ccgtgcctct gaagtccatc agaggccatc tccagggcta cagaatctac
     2581 tactggaaaa cccagagcag ctccaagcgg aacagacggc acatcgagaa gaagatcctg
     2641 acatttcagg gcagcaagac ccacggaatg ctgcctggcc tggaaccttt cagccactac
     2701 accctgaatg tgcgggtcgt gaacggcaaa ggcgagggac ctgcttctcc cgacagagtg
     2761 ttcaataccc cagagggcgt gccaagcgct ccttcctctc tgaaaatcgt gaaccctact
     2821 ctggacagcc tgacactgga atgggaccct ccatctcacc ccaacggcat cctgaccgag
     2881 tacacactga agtaccagcc tatcaacagc acccacgagc tgggccctct ggtggatctg
     2941 aagatccctg ccaacaagac caggtggacc ctgaagaatc tgaacttcag cacccggtac
     3001 aagttctact tctacgccca gacaagcgcc ggcagcggaa gccagattac tgaagaggcc
     3061 gtgaccacag tggacgaggc cggaattctg cctcctgatg ttggagccgg caaagtgcag
     3121 gctgtgaacc ccagaatcag caacctgaca gccgccgctg ccgaaaccta cgccaatatc
     3181 agctgggagt atgagggccc cgagcacgtg aacttctacg tggaatatgg cgtggccggc
     3241 agcaaagaag agtggcggaa agaaattgtg aacggctctc ggagcttctt cggcctgaag
     3301 ggactgatgc ctggcaccgc ctacaaagtt cgagtgggag ccgtgggcga tagcggcttt
     3361 gtgtctagcg aggacgtgtt cgagacaggc cctgccatgg cttccagaca ggtggacatt
     3421 gccacacaa
//
