LOCUS       sequence-303177-        9655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9655)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-MXRA5(Trunc2)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..9655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6997..7041
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7042..7059
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7199..7216)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7205..7316
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7434..7454)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7439..7768
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7619..7754
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7816..7863
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7882..8481
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7882..7901)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8378..8398
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8611..8732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8648..8667)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8702..8721
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8785..9576,9577..9645))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9408..9427
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9646..9655,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccaa aaaaggctct
     2761 ggcctgcctt ctaagagagg cagaaggcct ggcgccaaag ctctgagtag agtgcgggaa
     2821 gatatcgtgg aagatgaagg cggctctggc atgggcgacg aggaaaacac aagcagaagg
     2881 ctgctgcacc ccaaggacca agaggtgttc ctgaaaacaa aggacgacgc catcaacggc
     2941 gacaagaagg ccaagaaagg cagacggaag ctgaagctgt ggaagcacag cgagaaagaa
     3001 cccgagacaa acgtggccga gggcagaaga gtgtttgaga gccggcggag aatcaacatg
     3061 gccaacaagc agatcaaccc cgagagatgg gccgacattc tggccaaagt gcggggcaag
     3121 aatctgccta agggaaccga agtgcctcct ctgatcaaga ccacctctcc acctagcctg
     3181 agcctggaag tgacccctcc ttttcctgcc atctcacctc caagcgcctc tccagtgcag
     3241 acagtgacaa gcgccgagga atcttctgcc gatgtgcctc tgctgggaga agaggaacac
     3301 gtgctgggaa caatcagcag cgcctctatg ggcctcgagc acaaccataa tggcgtgatc
     3361 ctggtggaac ccgaagtgac cagcacacct ctggaagagg tggtggacga cctgagcgag
     3421 aaaaccgagg aaatcaccag caccgagggc gaccttaagg gaacagccgc tcctacactg
     3481 atcagcgagc cttacgagcc ctctccaaca ctgcacaccc tggacacagt gtacgagaag
     3541 cccacacacg aggaaacagc cactgaaggc tggtccgctg cagatgtggg aagctctcct
     3601 gagcctacaa gcagcgagta cgaacctcct ctggatgccg tgtctctggc cgagtctgag
     3661 cccatgcagt acttcgaccc cgacctggaa acaaagagcc agcctgacga ggacaaaatg
     3721 aaggaagata cgttcgccca cctgacgcct acacctacca tctgggtcaa cgatagcagc
     3781 accagccagc tgttcgagga tagcacaatt ggagagcctg gcgtgccagg acagtctcat
     3841 ctgcaaggcc tgaccgacaa catccacctg gtcaagagca gcctgtctac ccaggacacc
     3901 ctgctgatca aaaagggcat gaaggaaatg agccagacac tgcaaggcgg caacatgctg
     3961 gaaggcgacc ctacacacag cagatctagc gagagcgagg gccaagagag caagagcatc
     4021 accctgcctg atagcaccct gggcatcatg agcagcatga gccctgtgaa gaagcccgcc
     4081 gagacaacag tgggcacact gctggataag gataccacca ccgccaccac cacacctaga
     4141 cagaaagtgg cccctagctc caccatgagc acacacccta gcagaagaag gcctaacgga
     4201 aggcggagac tgcggcccaa caagttcaga cacagacaca aacagacccc tccaaccaca
     4261 ttcgccccta gcgagacatt cagcacccag cctacacagg cccctgacat caagatcagc
     4321 agccaggtgg aaagcagcct ggtgcctaca gcctgggtcg acaataccgt gaacacccct
     4381 aagcagctcg agatggaaaa gaacgccgag cctaccagca agggcacccc tagaagaaag
     4441 cacggcaagc ggcctaacaa gcacagatac acccctagca ccgtgtctag cagagccagc
     4501 ggaagcaagc cttctccatc tcctgagaac aagcaccgga acatcgtgac cccttccagc
     4561 gagacaatcc tgctgcctcg gacagtgtct ctgaaaacag agggccccta cgacagcctg
     4621 gactacatga ccaccaccag aaagatctac agcagctacc ccaaggtgca agagactctg
     4681 cccgtgacct acaagccaac cagcgacggc aaagaaatca aggacgatgt ggccaccaac
     4741 gtggacaagc acaagagcga tatcctggtc accggcgagt ccatcaccaa cgccattcca
     4801 acctccagat ctctggtgtc caccatgggc gagttcaaag aagagagcag cccagtcggc
     4861 ttccccggca cacctacatg gaatccaagc agaacagccc agcctggcag actgcagaca
     4921 ggcatccctg tgacaaccag cggcgagaat ctgaccgatc ctccactgct gaaagaactc
     4981 gaggacgtgg acttcaccag cgagtttctg tcctctctga ccgtgtccac acctttccac
     5041 caagaggaag ccggcagcag cacaaccctg tcctctatta aggtggaagt ggcctccagc
     5101 caggccgaga ctacaacact ggatcaggac catctggaaa ccaccgtggc catcctgctg
     5161 agcgaaacca gacctcagaa tcacacccct accgccgcta ggatgaagga acctgccagc
     5221 agctctccct ccaccatcct gatgtctctg ggccagacca caaccaccaa gcctgctctc
     5281 ccatctccaa gaatctccca ggccagccgg gacagcaaag aaaatgtgtt tctgaactac
     5341 gtgggcaacc ccgaaaccga ggccacacct gtgaacaatg agggcacaca gcacatgagc
     5401 ggccccaatg agctgagcac acctagcagc gaccaggacg ccttcaatct gagcaccaag
     5461 ctggaactgg aaaaacaggt gttcggcagc agaagcctgc ctagaggccc tgactctcag
     5521 agacaggatg gaagagtgca cgccagccac cagcttacca gagtgcctgc caaacctatc
     5581 ctgccaacag ctacagtgcg gctgcctgag atgtctacac agagcgccag ccggtacttc
     5641 gtgacctctc agtctcctcg gcactggacc aacaagcccg agatcacaac ataccctagc
     5701 ggagccctgc cagagaacaa acagttcaca acccctcggc tgtccagcac cacaattcct
     5761 ctgcctctgc acatgtccaa gcctagcatc cccagcaagt tcaccgacag acggaccgac
     5821 cagttcaacg gctacagcaa agtgttcgga aacaacaaca tccccgaggc cagaaatccc
     5881 gtgggcaagc caccttctcc tagaatccct cactacagca acggccggct gccattcttc
     5941 accaacaaga ccctgagctt ccctcagctg ggagtgacca gaaggccaca gatccctaca
     6001 tctcccgctc cagtgatgcg cgagagaaaa gtgatccccg gcagctacaa cagaatccac
     6061 agccacagca cctttcacct ggactttgga cctccagctc ctcctctgct gcacacacct
     6121 cagacaacag gcagcccaag caccaacctg cagaacatcc ctatggtgtc ctccacacag
     6181 agcagcatca gcttcatcac cagctctgtg cagagcagcg gctcctttca ccagagcagc
     6241 tccaagtttt ttgccggcgg acctcctgcc tccaagttct ggtccctggg agagaaaggc
     6301 gcgccgggcg gcgacaagac ccacacctgc cccccctgcc cagctccaga actgctggga
     6361 gggccttccg tgttcctgtt cccccccaag cccaaggaca ccctgatgat cagcaggacc
     6421 cccgaggtga cctgcgtggt ggtggccgtg tcccacgagg acccagaggt gaagttcaac
     6481 tggtacgtgg acggcgtgga ggtgcacaac gccaagacca agcccagaga ggagcagtac
     6541 aacagcacct acagggtggt gtccgtgctg accgtgctgc accaggactg gctgaacggc
     6601 aaagaataca agtgcaaagt ctccaacaag gccctggctg ccccaatcga aaagacaatc
     6661 agcaaggcca agggccagcc acgggagccc caggtgtaca ccctgccccc cagccgggag
     6721 gagatgacca agaaccaggt gtccctgacc tgtctggtga agggcttcta ccccagcgat
     6781 atcgccgtgg agtgggagag caacggccag cccgagaaca actacaagac caccccccca
     6841 gtgctggaca gcgacggcag cttcttcctg tacagcaagc tgaccgtgga caagtccagg
     6901 tggcagcagg gcaacgtgtt cagctgcagc gtgatgcacg aggccctgca caaccactac
     6961 acccagaagt ccctgagcct gagccccggc ggcagcggtc ttaatgatat tttcgaggct
     7021 cagaagattg agtggcatga acaccaccac catcaccact aaggccggcc tgtagtcact
     7081 ggcacaacgc gttacccggt aagccaatcg ggtatacacg gtcgtcatac tgcagacagg
     7141 gttcttctac tttgcaagat agtcttgagt agtaaaataa atagatagag aaaatcagcc
     7201 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7261 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcacaa
     7321 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7381 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     7441 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7501 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     7561 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7621 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7681 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     7741 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     7801 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     7861 gacaaggtga ggaactaaac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     7921 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     7981 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     8041 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     8101 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     8161 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     8221 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     8281 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     8341 gccggggtgc ccgccttcct ggagacatcc gcgccccgca acctcccctt ctacgagcgg
     8401 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     8461 acccgcaagc ccggtgcctg acacgtgcta cgagatttcg attccaccgc cgccttctat
     8521 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8581 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     8641 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8701 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     8761 tagagcttgg cgtaatcatg gtcattacca atgcttaatc agtgaggcac ctatctcagc
     8821 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8881 acgggagggc ttaccatctg gccccagcgc tgcgatgata ccgcgagaac cacgctcacc
     8941 ggctccggat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9001 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9061 ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct acaggcatcg tggtgtcacg
     9121 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9181 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9241 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9301 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9361 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9421 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9481 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9541 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9601 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatattct tcctt
//
