LOCUS       sequence-303180-        3579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NFASC-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..3579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attgagatcc ctatggaccc ctctatccag aacgagctga cccagcctcc taccatcaca
       61 aagcagagcg ccaaggacca catcgtggac cccagagaca acatcctgat cgagtgcgag
      121 gccaagggca accctgctcc tagcttccac tggacccgga acagccggtt cttcaatatc
      181 gccaaagatc cccgggtgtc catgagaagg cgctctggca cactggtcat cgactttaga
      241 agcggcggca gacctgagga atacgagggc gagtaccagt gcttcgcccg gaacaaattt
      301 ggcacagccc tgagcaaccg gatcagactc caggtgtcca agtctcccct gtggcctaaa
      361 gaaaatctgg accccgtggt ggtgcaagag ggtgctcctc tgactctgca gtgcaatcct
      421 cctcctggac tgccctctcc tgtgatcttt tggatgagca gcagcatgga acccatcaca
      481 caggacaaga gagtgtccca gggccacaac ggcgacctgt acttcagcaa cgtgatgctg
      541 caggacatgc agaccgacta cagctgcaac gccagattcc acttcaccca caccatccag
      601 cagaagaacc ccttcacact gaaggtgctg accacaagag gcgtggccga gagaacccct
      661 agcttcatgt atcctcaggg caccgccagc agccagatgg tgcttagagg aatggacctg
      721 ctgctggaat gtatcgccag cggcgtgcca acacctgata tcgcctggta caagaaaggc
      781 ggcgatctgc ccagcgacaa ggccaagttc gagaacttca acaaggccct gcggatcacc
      841 aacgtgtccg aggaagatag cggcgagtac ttctgcctgg ccagcaacaa gatgggcagc
      901 atcagacaca ccatcagcgt cagagtgaag gccgctcctt actggctgga cgagcccaag
      961 aatctgattc tggcccctgg cgaagatggc agactcgtgt gtagagccaa cggcaatcct
     1021 aagcctaccg tgcagtggat ggtcaacggc gaacctctgc agtctgcccc tcctaatcct
     1081 aatagagagg tggccggcga caccatcatc ttcagggata cccagatcag cagcagagcc
     1141 gtgtaccagt gtaacaccag caacgagcac ggctacctgc tggccaatgc tttcgtgtct
     1201 gtgctggacg tgccacctag aatgctgagc cccagaaacc agctgatccg cgtgatcctg
     1261 tacaacagaa cccggctgga ttgcccattc ttcggcagcc ccattcctac actgcggtgg
     1321 tttaagaacg gccagggctc taacctggac ggcggcaatt accacgtgta cgagaacggc
     1381 agcctggaaa tcaagatgat ccggaaagag gaccagggca tctacacatg cgtggccacc
     1441 aatatcctgg gcaaagccga gaatcaagtg cggctggaag tgaaggaccc caccagaatc
     1501 tacagaatgc ccgaggatca ggtggccaga cggggaacaa cagtgcagct ggaatgcaga
     1561 gtgaaacacg accccagcct gaagctgacc gtgtcctggc tgaaggacga cgagcctctg
     1621 tacatcggca acaggatgaa gaaagaggat gacagcctga ccatcttcgg agtggccgaa
     1681 agagatcagg gctcctatac ctgtgtggcc agcaccgaac tggaccagga tctggccaaa
     1741 gcctatctga cagtgctggc cgatcaggcc acacctacaa atagactggc cgctctgcct
     1801 aagggcagac ccgatagacc tagagatctg gaactgaccg acctggctga gagatctgtg
     1861 cggcttacat ggattcccgg cgacgccaac aacagcccca tcacagatta cgtggtgcag
     1921 ttcgaagagg atcagtttca gcccggcgtg tggcacgatc acagcaagta tcctggctcc
     1981 gtgaacagcg ccgtgctgag actgagccct tacgtgaact accagttcag agtgatcgcc
     2041 atcaatgaag tgggcagctc tcaccctagc ctgcctagcg agagatacag aacaagcgga
     2101 gcccctcctg agagcaaccc tggggatgtg aaaggcgagg gcaccagaaa gaacaacatg
     2161 gaaatcacct ggacacccat gaacgccacc agcgcctttg gacccaacct gcggtatatt
     2221 gtgaagtggc ggcggagaga gacacgcgag gcttggaaca atgtgaccgt gtggggcagc
     2281 agatacgtcg tgggacagac acctgtgtac gtgccctacg agattagagt gcaggccgag
     2341 aacgatttcg gcaagggacc tgagcctgag agcgtgatcg gatacagcgg cgaggattat
     2401 cctagagccg ctcctaccga agtgaaagtg cgcgtgatga acagcacagc catcagcctg
     2461 cagtggaaca gagtgtacag cgacacagtg cagggccagc tgagagagta ccgggcctac
     2521 tattggagag agagcagcct gctgaagaac ctgtgggtgt cccagaagag acagcaggcc
     2581 agctttcctg gcgatagact gagaggcgtc gtgtccagac tgttccccta ctccaactac
     2641 aagctggaaa tggtggtcgt gaacggcaga ggcgacggcc ctagaagcga gacaaaagag
     2701 tttaccacac ctgagggcgt gcccagcgct cctagaaggt ttagagtgcg gcagcccaac
     2761 ctggaaacca tcaatctgga atgggatcac cctgagcacc ccaacggcat catgatcggc
     2821 tacaccctga aatacgtggc cttcaacggc accaaagtgg gcaagcagat cgtggaaaac
     2881 ttcagcccca accagaccaa gttcaccgtg cagagaaccg atccagtgtc cagataccgg
     2941 ttcaccctgt ccgccagaac acaagttgga tctggcgagg ccgtgaccga ggaatctcct
     3001 gctcctccaa atgaggccac tccaacagct gctccaccaa cactgccacc tacaacagtt
     3061 ggagccacag gcgccgtgtc tagcacagat gctacagcca ttgccgccac cacagaggcc
     3121 acaaccgtgc ctattattcc cacagtggcc cctaccacaa tcgccacaac aaccaccgtg
     3181 gctaccacca caactaccac agccgctgcc acaaccacaa cagagagccc tccaacaaca
     3241 acctccggca ccaagatcca cgagagcgcc cctgatgagc agtccatttg gaatgtgact
     3301 gtgctgccta acagcaagtg ggccaacatc acatggaagc acaacttcgg ccctggcacc
     3361 gacttcgtgg tcgagtacat cgacagcaac cacaccaaaa agaccgtgcc tgtgaaggcc
     3421 caggctcagc ctattcagct gaccgatctg taccccggca tgacctacac actgcgggtg
     3481 tacagcagag acaatgaggg catcagctcc accgtgatca ccttcatgac cagcaccgcc
     3541 tacaccaaca accaggccga tattgccaca caaggctgg
//
