LOCUS       sequence-303186-        3759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PTPRS-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..3759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(202..219)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gaagaacctc ctagattcat caaagagccc aaggaccaga tcggcgttag cggcggagtt
       61 gccagttttg tgtgtcaggc cacaggcgac cctaagccta gagtgacctg gaacaagaag
      121 ggcaagaaag tcaacagcca gcgcttcgag acaatcgagt tcgatgaatc tgccggcgct
      181 gtgctgagaa tccagcctct gagaacccct cgggacgaga atgtgtatga gtgcgtggcc
      241 cagaacagcg tgggagagat tacagtgcac gccaagctga ccgtgctgag agaggatcag
      301 ctgcccagcg gcttccccaa cattgatatg ggacctcagc tgaaggtggt ggaaagaacc
      361 agaaccgcca caatgctgtg tgccgcctct ggcaatcctg atcctgagat tacctggttc
      421 aaggacttcc tgcctgtgga ccctagcgcc agcaatggca gaatcaagca gctgcggagc
      481 gagacattcg agagcacacc tattagaggc gccctgcaga tcgagagcag cgaggaaaca
      541 gaccagggca aatacgagtg tgtggccacc aatagcgctg gcgtgcggta ctctagccct
      601 gccaatctgt atgtgcgcga gctgagagaa gtgcggagag tggccccaag attcagcatc
      661 ctgcctatga gccacgagat catgcctggc ggcaacgtga acatcacatg tgtggccgtg
      721 ggcagcccca tgccttatgt gaaatggatg caaggcgccg aggatctgac ccctgaggat
      781 gatatgcctg tgggcagaaa cgtgctggaa ctgaccgatg tgaaggacag cgccaactac
      841 acatgcgtgg ccatgtctag cctgggcgtg atcgaagccg tggctcagat cacagtgaag
      901 tccctgccta aggctcctgg cacacccatg gttacagaga acaccgccac cagcatcacc
      961 atcacctggg atagcggaaa ccccgatcct gtgtcctact acgtgatcga gtacaagagc
     1021 aagagccagg acggccccta ccagatcaaa gaggacatca ccaccaccag atacagcatc
     1081 ggcggactga gccccaacag cgagtatgag atttgggtgt ccgccgtgaa ctccatcgga
     1141 cagggacctc catctgagag cgtggtcaca agaacaggcg aacaggctcc tgccagcgct
     1201 cctagaaatg tgcaggccag aatgctgagc gccaccacca tgatcgtgca gtgggaagaa
     1261 cccgtggaac ccaatggcct gatccggggc tacagagtgt actacaccat ggaacctgag
     1321 caccccgtcg gcaactggca gaaacacaat gtggacgaca gcctgctgac caccgtggga
     1381 tctctgctgg aagatgagac atacaccgtg cgcgtgctgg cctttacatc tgttggagat
     1441 ggccctctga gcgaccccat ccaagtgaaa acacagcagg gcgtgcccgg ccagcctatg
     1501 aatcttagag ccgaggccag aagcgagact agcatcacac ttagctggtc cccacctcgg
     1561 caagagagca tcattaagta cgagctgctg ttcagagagg gcgaccacgg aagagaagtc
     1621 ggcagaacct ttgatcccac cacctcctac gtggtggaag atctgaagcc caataccgag
     1681 tacgccttca gactggccgc cagatctcca caaggactgg gcgcttttac acccgtcgtc
     1741 agacagagaa ccctgcagtc taaaccctcc gctccacctc aggatgtgaa gtgcgtgtcc
     1801 gtgcggtcta cagccattct ggtttcttgg cggcctcctc cacctgagac acataatggc
     1861 gccctcgtgg gctactctgt gcggtataga cctctgggca gcgaagatcc cgagcctaaa
     1921 gaagtgaacg gcatccctcc taccacaaca cagattctgc tcgaggccct ggaaaagtgg
     1981 acccagtaca gaatcaccac agtggcccac accgaagttg gccctggacc tgaatcttct
     2041 cctgtggtcg tgcggaccga tgaggatgtt cctagcgctc ctcctaggaa ggtggaagcc
     2101 gaagctctga atgccaccgc catcagagtg ctttggagaa gccctgctcc aggcagacag
     2161 cacggccaga ttagaggcta ccaggtgcac tatgtgcgga tggaaggcgc tgaagccaga
     2221 ggacctccac ggatcaagga cgttatgctg gccgatgctc agtgggagac agacgataca
     2281 gccgagtacg agatggtcat caccaacctg cagcctgaga cagcctacag cattaccgtg
     2341 gccgcctaca caatgaaagg cgacggcgcc agatccaagc ctaaggtggt cgtgacaaaa
     2401 ggcgcagtgc tgggcagacc tacactgtct gttcagcaga cacctgaggg cagtctgctg
     2461 gctagatggg aacctcctgc cggaacagct gaagatcagg tgctgggata cagactgcag
     2521 ttcggcagag aggatagcac ccctctggcc acactggaat tccctccaag cgaggataga
     2581 tacaccgcca gcggagtgca caaaggcgcc acatacgtgt tcaggctggc tgctagaagc
     2641 agaggcggac ttggagaaga ggccgccgag gttctgtcta tcccagagga tacacctcgg
     2701 ggacaccctc agattctgga agccgccgga aatgcttctg ctggcacagt tctgctgaga
     2761 tggctgcctc ctgtgcctgc cgagagaaat ggcgctatcg tgaagtacac tgtggccgtc
     2821 agagaagctg gtgccctggg acctgctaga gagacagaac ttcctgctgc tgccgagcct
     2881 ggcgccgaaa atgctcttac actgcaagga ctgaagcccg acaccgccta tgatctgcaa
     2941 gtgcgggccc acacaagaag aggacctgga ccttttagcc caccagtgcg gtacaggacc
     3001 ttcctgagag atcaagtgtc ccctaagaac ttcaaagtga agatgatcat gaagaccagc
     3061 gtgctgctga gctgggagtt ccccgacaac tacaacagcc ccacacctta caagatccag
     3121 tacaacggac tgaccctgga cgtggacggc aggaccacaa agaagctgat cacccacctg
     3181 aagccacaca ccttctacaa cttcgtgctg accaacagag gcagctctct cggaggactg
     3241 cagcagacag ttacagcctg gaccgccttc aacctgctga atggcaaacc tagcgtggcc
     3301 cctaaacctg acgccgatgg cttcatcatg gtgtacctgc ctgatggcca gtctccagtg
     3361 cctgtgcaga gctacttcat cgtgatggtg cccctgagaa agagtagagg cgggcagttt
     3421 ctgaccccac tgggatctcc cgaggacatg gatctggaag aactgatcca ggacatctcc
     3481 cggctgcaga gaagaagcct gaggcacagt agacagctgg aagtgcccag accttatatc
     3541 gccgccaggt tttctgtgct gcctccaaca tttcaccccg gcgatcagaa gcagtacggc
     3601 ggctttgaca acagaggact ggaacccggc cacagatacg tgctgtttgt gctggcagtg
     3661 ctgcagaagt ccgagcctac attcgccgcc tctccattca gcgacccctt tcagctggac
     3721 aaccctgatc cacagcctat cgtggatggc gaagagggt
//
