LOCUS       sequence-303187-        9886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PAPLN(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..9886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             7228..7272
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7273..7290
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7430..7447)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7436..7547
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7665..7685)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7670..7999
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7850..7985
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7912..7931
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8047..8094
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8113..8712
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8113..8132)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8609..8629
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8842..8963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8879..8898)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8933..8952
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9016..9807,9808..9876))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9639..9658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9877..9886,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccgc tcctaaagtt
     2761 cggagacagt ctgatacctg gggaccttgg agtcagtggt ccccttgcag cagaacatgt
     2821 ggcggcggag tgtccttcag agagaggcct tgttacagcc agcggagaga tggcggcagc
     2881 tcttgtgttg gacctgccag aagccacaga agctgcagaa ccgagagctg ccctgatggc
     2941 gccagagatt tcagagccga gcagtgcgcc gagttcgatg gcgctgaatt ccaaggcaga
     3001 cggtacagat ggctgcccta ctacagcgcc cctaacaagt gcgagctgaa ctgcatcccc
     3061 aagggcgaga acttctacta caagcaccgg gaagccgtgg tggatggcac cccttgtgaa
     3121 cctggcaaga gggatgtgtg tgtggacggc agctgtagag tcgtgggctg tgatcacgag
     3181 ctggacagca gcaagcaaga ggacaagtgc ctgagatgcg gaggcgacgg caccacatgt
     3241 tatcctgtgg ccggcacctt cgacgccaac gatcttagca gaggctacaa ccagatcctg
     3301 atcgtgccta tgggcgccac cagcattctg atcgatgaag ccgctgccag ccggaacttt
     3361 ctggccgtga aaaatgtgcg gggcgagtac tacctgaacg gccactggac aatcgaggcc
     3421 gctagagcac ttcctgccgc ctctacaatc ctgcactatg agagaggcgc cgagggcgat
     3481 ctggctcctg aaagacttca tgccagagga cccaccagcg agcctctggt tatcgagctg
     3541 atctcccaag agcctaatcc tggcgtgcac tacgagtacc atctgcctct gagaaggccc
     3601 tctcctggct tctcttggag ccacggatct tggagcgatt gctctgccga atgtggcgga
     3661 ggacaccaga gcagactggt gttctgcacc atcgaccacg aggcttaccc cgaccacatg
     3721 tgccagagac agcctagacc tgccgacagg cggagctgta atctgcaccc ttgtcctgag
     3781 acaaagcggt ggaaagccgg accttgggct ccttgttctg cctcttgcgg cggaggatct
     3841 cagtccagaa gcgtgtactg catcagctcc gatggcgccg gaatccaaga ggctgtggaa
     3901 gaggctgaat gtgccggcct gcctggaaag cctcctgcca ttcaggcttg caacctgcag
     3961 agatgtgccg cttggagccc tgagccttgg ggcgaatgtt ctgtgtcttg tggcgtgggc
     4021 gtcagaaagc ggagcgtgac atgcagaggc gagagaggat ctctgctgca cacagccgcc
     4081 tgcagcctgg aagatagacc tcctctgacc gagccttgcg tgcacgagga ttgtcctctg
     4141 ctgtctgatc aggcctggca cgttggaaca tggggcctgt gtagcaagag ctgtagcagc
     4201 ggaacccggc ggagacaagt gatctgtgcc atcggacctc caagccactg tggaagcctg
     4261 cagcacagca agcccgtgga tgtggaacct tgcaacaccc agccttgcca tctgccacaa
     4321 gaggtgccca gcatgcagga tgtgcacacc cctgccagca atccctggat gccactggga
     4381 cctcaagaga gcccagccag cgatagtaga ggacagtggt gggccgctca agagcaccct
     4441 tctgctagag gcgatcacag aggcgaaagg ggcgatccca ggggagatca aggcacacat
     4501 ctgtctgctc tcggccctgc accttctctg cagcagcctc cttatcagca gccactgaga
     4561 agcggctctg gccctcacga ttgcagacac tctccacacg gctgttgccc agatggccac
     4621 acagcatctc tgggccctca atggcaagga tgtcctggcg ctccttgcca gcagagcaga
     4681 tacggatgct gccccgatag agtgtccgtg gccgaaggac ctcatcacgc cggctgtaca
     4741 aagagctacg gcggcgattc tacaggcggc atgcctagaa gtagagccgt ggccagcacc
     4801 gtgcacaaca ctcatcagcc tcaggctcag cagaacgagc cctctgagtg tagaggcagc
     4861 cagttcggct gctgctacga caatgtggct acagccgctg gacctctcgg cgaaggatgt
     4921 gtgggacagc cttctcacgc ttaccctgtg cgttgtctgc tgccttctgc tcacggctct
     4981 tgtgccgatt gggccgccag atggtatttc gtggcctctg tgggccagtg caaccggttt
     5041 tggtacggcg gctgtcacgg caacgccaac aatttcgcca gcgagcaaga gtgcatgagc
     5101 agctgtcagg gctctctgca cggacctaga aggcctcaac ctggcgcctc tggcagatct
     5161 acacacacag atggtggcgg atctagccct gccggcgaac aagaacctag ccagcataga
     5221 acaggcgccg ctgtgcagag gaagccttgg ccttctggcg gactttggag acaggaccag
     5281 caacctggac ctggcgaagc ccctcacaca caggcttttg gagaatggcc atggggccaa
     5341 gagctgggat caagagcccc tggacttggc ggagatgctg gatctcctgc tcctccattt
     5401 cacagcagca gctacagaat cagcctggcc ggcgtggaac catctctggt tcaagctgct
     5461 ctgggacagc tcgtgcggct gagctgttcc gatgatacag cccctgaatc tcaggccgcc
     5521 tggcagaaag atggccagcc aatcagcagc gaccggcaca gactgcagtt tgacggcagc
     5581 ctgatcattc acccactgca ggctgaggac gccggaacat atagctgcgg ctctaccaga
     5641 cctggccggg actctcagaa gatccagctg agaatcatcg gcggcgacat ggccgttctg
     5701 tctgaggccg aactgagcag attccctcag cctagagatc ccgctcagga ttttggacag
     5761 gctggcgctg ctggaccact gggagctatt ccatcttctc accctcagcc agccaaccgg
     5821 ctgagactgg accagaatca acccagagtg gtggacgcct ctccaggcca gagaatcaga
     5881 atgacctgta gagccgaggg cttcccacca ccagctatcg agtggcaacg agatgggcag
     5941 cctgtgtcta gcccaagaca tcagctgcag ccagatggca gcctcgtgat tagcagagtg
     6001 gccgtggaag atggcggatt ctacacatgc gtggccttca acggccagga ccgggatcag
     6061 agatgggtgc agctgagagt gctgggcgag ctgacaattt ctggcctgcc tcctaccgtg
     6121 acagtgccag agggcgatac agccagactg ctgtgtgtgg tggctggcga gtccgtgaac
     6181 atccggtggt ccagaaatgg cctgccagtg caggccgatg gccatagagt tcaccagtct
     6241 ccagacggca ccctgctgat ctacaacctg agagccagag atgagggctc ctacacctgt
     6301 agcgcctacc agggttctca ggctgtgtcc agatccaccg aagtgaaggt ggtgtcccct
     6361 gctcctacag cacagccacg agatcccggc agagactgtg tggatcaacc cgagctggcc
     6421 aactgcgacc tgattctgca ggcccagctg tgcggcaatg agtactacag cagcttttgc
     6481 tgcgccagct gctccagatt ccagccacac gctcagccca tctggcaagg cgcgccgggc
     6541 ggcgacaaga cccacacctg ccccccctgc ccagctccag aactgctggg agggccttcc
     6601 gtgttcctgt tcccccccaa gcccaaggac accctgatga tcagcaggac ccccgaggtg
     6661 acctgcgtgg tggtggccgt gtcccacgag gacccagagg tgaagttcaa ctggtacgtg
     6721 gacggcgtgg aggtgcacaa cgccaagacc aagcccagag aggagcagta caacagcacc
     6781 tacagggtgg tgtccgtgct gaccgtgctg caccaggact ggctgaacgg caaagaatac
     6841 aagtgcaaag tctccaacaa ggccctggct gccccaatcg aaaagacaat cagcaaggcc
     6901 aagggccagc cacgggagcc ccaggtgtac accctgcccc ccagccggga ggagatgacc
     6961 aagaaccagg tgtccctgac ctgtctggtg aagggcttct accccagcga tatcgccgtg
     7021 gagtgggaga gcaacggcca gcccgagaac aactacaaga ccaccccccc agtgctggac
     7081 agcgacggca gcttcttcct gtacagcaag ctgaccgtgg acaagtccag gtggcagcag
     7141 ggcaacgtgt tcagctgcag cgtgatgcac gaggccctgc acaaccacta cacccagaag
     7201 tccctgagcc tgagccccgg cggcagcggt cttaatgata ttttcgaggc tcagaagatt
     7261 gagtggcatg aacaccacca ccatcaccac taaggccggc ctgtagtcac tggcacaacg
     7321 cgttacccgg taagccaatc gggtatacac ggtcgtcata ctgcagacag ggttcttcta
     7381 ctttgcaaga tagtcttgag tagtaaaata aatagataga gaaaatcagc ctcgactgtg
     7441 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7501 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcaca acactcaacc
     7561 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7621 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     7681 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7741 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7801 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7861 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7921 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     7981 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     8041 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     8101 aggaactaaa ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     8161 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     8221 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     8281 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     8341 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     8401 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     8461 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     8521 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     8581 cccgccttcc tggagacatc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     8641 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     8701 cccggtgcct gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     8761 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8821 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     8881 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8941 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     9001 gcgtaatcat ggtcattacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9061 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9121 cttaccatct ggccccagcg ctgcgatgat accgcgagaa ccacgctcac cggctccgga
     9181 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9241 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9301 taatagtttg cgcaacgttg ttgccatcgc tacaggcatc gtggtgtcac gctcgtcgtt
     9361 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9421 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9481 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9541 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9601 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9661 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9721 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9781 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9841 gggaataagg gcgacacgga aatgttgaat actcatattc ttcctt
//
