LOCUS       sequence-303190-        4272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NOTCH4(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..4272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agaggactgc tgtgtggatc ttttccagag ccttgcgcca atggcggcac atgtctgtct
       61 ctgtctctcg gccagggaac ctgtcagtgt gctcctggat ttctgggcga gacttgtcag
      121 ttccccgatc cttgtcagaa cgcccagctg tgtcagaatg gcggctcttg tcaagctctg
      181 ctgcctgctc ctctgggact gccttcttct ccatctccac tgacacccag ctttctgtgc
      241 acctgtctgc ctggctttac cggcgaaaga tgccaggcca agctggaaga tccctgtcct
      301 cctagcttct gcagcaagag gggcagatgt cacatccagg caagcggcag acctcagtgt
      361 tcttgtatgc ctggctggac aggcgagcag tgccagctga gagatttctg cagcgccaat
      421 ccttgtgtga acggcggcgt gtgtctggcc acatatcctc agatccagtg ccactgtcct
      481 ccaggctttg agggacacgc ctgcgagagg gatgtgaacg agtgttttca ggaccccgga
      541 ccttgtccta agggcacctc ttgtcacaac accctgggca gcttccagtg tctgtgtcct
      601 gtgggacaag agggacccag atgtgaactg agagccgggc cttgtccacc tagaggctgt
      661 tctaatggcg ggacctgtca gctgatgccc gagaaggata gcaccttcca cctgtgtctg
      721 tgcccacctg gcttcatcgg ccccgattgc gaagtgaacc ccgacaattg tgtgtcccac
      781 cagtgccaga acggcggaac ttgtcaggac ggcctggaca cctatacctg cctgtgtcca
      841 gagacatgga ccggctggga ttgcagcgag gatgtggatg agtgtgaaac acagggccct
      901 ccacactgca gaaacggcgg gacatgccag aatagcgccg gctcttttca ctgcgtgtgt
      961 gtgtcaggct ggggcggcac aagctgcgag gaaaatctgg acgattgcat tgccgccaca
     1021 tgcgcccctg gcagcacatg tattgataga gtgggctcct tcagctgtct ctgccctcct
     1081 ggaagaacag gcctgctgtg tcacctggaa gatatgtgcc tgagccagcc ttgtcatggc
     1141 gacgcccagt gtagcacaaa tcctctgacc ggcagcaccc tgtgtctttg tcagcctggc
     1201 tactctggcc ctacctgcca ccaggatctg gatgaatgtc tgatggccca gcagggccct
     1261 tctccttgtg aacatggcgg aagctgtctg aacacacccg gcagcttcaa ctgcttgtgt
     1321 cctcctggct acaccggaag cagatgcgag gccgatcaca acgaatgcct gtctcagcct
     1381 tgccatcctg gctctacctg tctggatctg ctggccacct ttcactgcct ctgtccgcct
     1441 ggactggaag gacagctgtg cgaggtggaa acaaatgagt gcgccagcgc tccttgcctg
     1501 aatcacgccg attgccacga cctgctgaac ggctttcagt gtatctgcct gcctggattc
     1561 agcggcacca gatgcgaaga ggacatcgac gagtgtagaa gcagcccttg tgccaacggt
     1621 ggccagtgtc aagatcagcc aggcgccttc cactgcaagt gtctgccagg atttgaaggc
     1681 cccagatgcc agaccgaagt ggacgagtgt ctgagcgatc catgtccagt gggagcctct
     1741 tgtcttgacc tgcctggcgc cttcttttgt ctttgcccta gcggctttac agggcagctg
     1801 tgtgaagtgc ccctgtgcgc tcctaatctg tgccagccta aacagatctg caaggaccag
     1861 aaggataagg ccaactgcct ttgtcctgat ggctctccag gatgcgcccc acctgaggat
     1921 aattgcacat gtcaccacgg ccactgccag agaagctctt gtgtgtgtga tgtcggatgg
     1981 acaggacccg agtgtgaagc cgaactcggc ggatgtatta gcgccccatg tgctcacggt
     2041 ggcacctgtt atccacagcc aagcggctac aactgcacat gccctacagg ctacacaggc
     2101 ccaacctgct ctgaggaaat gacagcctgt cacagcggcc catgccttaa cggcgggtct
     2161 tgtaatcctt ctcctggcgg ctactactgt acctgtccac catctcacac cgggcctcag
     2221 tgtcagacaa gcaccgacta ctgtgtgtct gccccttgct ttaacggtgg aacctgcgtg
     2281 aacagacctg gcacattctc ttgcctgtgc gccatgggct ttcaggggcc tagatgtgaa
     2341 ggcaagctga ggccttcctg cgccgactct ccctgtagaa atagagccac ctgtcaggac
     2401 agccctcagg gcccaagatg tttgtgtcca accggctata ccggcggcag ctgtcagaca
     2461 ctgatggacc tgtgtgccca gaagccttgt cctcggaata gccactgtct gcaaacaggc
     2521 ccctccttcc attgcctctg cctgcaagga tggaccgggc cactgtgtaa tctgcctctc
     2581 agctcctgtc agaaggccgc actgtctcag ggcatcgatg tgtccagtct ctgccacaat
     2641 ggcggactgt gtgttgatag cggccccagc tacttctgtc actgcccacc aggttttcag
     2701 ggctccctgt gtcaggatca cgtgaaccct tgcgagagca gaccttgcca gaatggggcc
     2761 acttgtatgg ctcagccttc tggctatctg tgtcagtgcg ccccaggcta cgatggccag
     2821 aattgcagca aagagctgga cgcctgtcag agccagccat gccacaatca cggcacatgc
     2881 acacctaagc ctggcggatt tcactgtgca tgccctccag gattcgtggg acttcgctgt
     2941 gaaggcgacg ttgacgaatg tctggaccag ccatgtcacc ctactggaac agccgcctgt
     3001 cattctctgg ccaacgcctt ctattgccag tgtctccctg gacacacagg ccagtggtgc
     3061 gaggtcgaga ttgatccttg ccactctcag ccctgctttc acggcggtac atgtgaagct
     3121 acagccggct ctcccctggg cttcatttgt cactgtccca agggattcga gggccccaca
     3181 tgttctcaca gagcccctag ctgtggcttc caccactgtc atcacggcgg actctgtctg
     3241 ccatctccta agccaggctt cccacctaga tgcgcctgtc tctctggata tggcggccct
     3301 gattgtctga cccctcctgc tcctaaaggc tgtggccctc cttctccatg cctgtacaat
     3361 ggcagctgta gcgagacaac aggcctcggc ggaccaggct ttagatgtag ctgtcctcac
     3421 agcagccctg gaccaaggtg tcagaaacct ggcgctaagg gctgcgaagg cagatctggc
     3481 gacggtgcat gtgatgctgg atgttctgga cctggcggaa actgggatgg cggagattgc
     3541 tctctgggag tccctgatcc ttggaaggga tgccctagcc acagcagatg ctggctgctg
     3601 ttcagagatg gccagtgcca tcctcagtgc gacagcgaag agtgcctgtt cgatggctac
     3661 gactgcgaga caccaccagc ctgtacaccc gcctacgatc agtactgcca cgatcacttc
     3721 cacaacggac actgcgagaa gggctgcaat accgccgaat gtggatggga cggtggcgat
     3781 tgcagaccag aggatggcga tcctgaatgg ggaccttctc tcgctctgct ggttgtgctt
     3841 agccctccag ctctggatca gcagctgttt gctctggcta gagtgctgag cctgactctg
     3901 agagttggcc tgtgggtccg aaaggaccgc gacggcagag atatggtgta cccttatcca
     3961 ggcgccagag ccgaggaaaa gctcggcgga acaagagatc ccacctacca agaaagagcc
     4021 gctcctcaga cacagcccct gggcaaagag acagattccc tgagcgccgg atttgtggtg
     4081 gtcatgggag tcgatctgtc cagatgtgga cctgatcacc ccgccagtag atgtccttgg
     4141 gaccctggac tgctgctgag attccttgct gctatggctg ccgtgggagc acttgaacca
     4201 ttgctgcctg gaccactgct ggctgtgcat cctcatgctg gaaccgctcc tccagctaac
     4261 cagcttcctt gg
//
