LOCUS       sequence-303191-       10426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PXDNL(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..10426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             7768..7812
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7813..7830
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7970..7987)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7976..8087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8205..8225)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8210..8539
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8390..8525
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8452..8471
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8587..8634
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8653..9252
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8653..8672)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9149..9169
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9382..9503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9419..9438)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9473..9492
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9556..10347,10348..10416))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10179..10198
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10417..10426,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgcctg tcctagcaga
     2761 tgcctgtgtt ttaagagcac cgtgcgctgc atgcacctga tgctggatca cattcctcag
     2821 gtgccacagc agaccaccgt gctggacctg cggttcaaca gaatcagaga gatccccggc
     2881 agcgccttca agaagctgaa gaacctgaac accctgctgc tgaacaacaa ccacatccgg
     2941 aagatcagcc ggaacgcctt tgaaggcctg gaaaacctgc tgtacctgta cctctacaag
     3001 aacgagattc acgccctgga caagcagacc ttcaagggcc tgatttccct ggaacacctg
     3061 tacatccact tcaaccagct ggaaatgctg cagcccgaga cattcggcga cctgctgaga
     3121 ctggaacggc tgttcctgca caacaacaag ctgagcaaga tcccagccgg cagcttcagc
     3181 aacctggaca gcctgaaaag actgcggctg gacagcaatg ccctcgtgtg cgattgtgat
     3241 ctgatgtggc tgggagagct gctgcaggga tttgcccagc atggacatac acaggccgct
     3301 gccacctgtg aataccctag aaggctgcat ggcagagccg tggcctctgt gaccgtggaa
     3361 gagttcaact gtcagagccc cagaatcacc ttcgagcccc aagatgtgga agtgcccagc
     3421 ggcaacaccg tgtactttac atgcagagcc gagggcaacc ccaagcctga gatcatctgg
     3481 attcacaaca atcacagcct ggacctggaa gatgacaccc ggctgaacgt gttcgacgac
     3541 ggcaccctga tgatccggaa caccagagaa agcgatcagg gcgtgtacca gtgcatggcc
     3601 agaaattctg ccggcgaggc caagacacag agcgccatgc tgagatacag cagcctgcct
     3661 gccaagccta gcttcgtgat tcagcctcag gacaccgagg tgctgatcgg cacaagcacc
     3721 acactggaat gcatggctac cggccatcct catcctctga tcacctggac cagagacaac
     3781 ggcctggaac tggatggaag cagacacgtg gcaaccagct ctggcctgta cctgcagaac
     3841 atcacccaac gcgaccacgg cagattcacc tgtcacgcca acaatagcca cggcacagtg
     3901 caggctgccg ccaatattat cgttcaggcc cctcctcagt tcaccgtgac acctaaagat
     3961 caggtggtgc tggaagaaca cgccgtggaa tggctgtgcg aggccgatgg aaatcctcct
     4021 cctgtgatcg tgtggaccaa gacaggtgga cagctgccag tggaaggaca gcacacagtg
     4081 ctgtctagcg gcacactgag aatcgacaga gccgctcagc acgaccaggg ccagtatgaa
     4141 tgtcaagccg tgtccagcct gggcgtgaag aaagtgtctg tgcagctgac cgtgaagccc
     4201 aaggctctgg ctgtgtttac ccagctgcct caggatacaa gcgtggaagt gggcaagaac
     4261 atcaacatca gctgccacgc tcagggcgag cctcagccta tcatcacatg gaacaaagaa
     4321 ggcgtgcaga tcaccgagag cggcaagttt catgtggacg acgagggaac cctgaccatc
     4381 tacgatgccg gctttccaga ccagggcaga tacgagtgtg tggcccggaa ttcttttggc
     4441 ctggccgtga ccaacatgtt cctgacagtg accgccattc agggcagaca ggccggcgac
     4501 gattttgtgg aaagcagcat tctggacgcc gtgcagagag tggatagcgc catcaatagc
     4561 accagacggc acctgttcag ccagaagcct cacacatcta gcgatctgct ggcccagttt
     4621 cactacccta gggaccctct gatcgtggaa atggccagag ccggcgagat ctttgagcac
     4681 acactgcagc tgatccgcga gagagtgaag cagggactga cagtggatct ggaaggcaaa
     4741 gagttccggt acaacgacct ggtgtcccct agaagcctga gcctgatcgc caatctgagc
     4801 ggatgtactg ccagaaggcc cctgcctaac tgcagcaata gatgcttcca cgccaagtac
     4861 agagcccacg acggaacctg caacaacctc cagcaaccta catggggagc cgctctgact
     4921 gcctttgcta gactgctcca gccagcctac agagatggaa tcagagcacc tagaggcctg
     4981 ggactgcctg ttggaagtag acaacctctg cctcctccta gactggtggc cacagtttgg
     5041 gctagagctg ccgctgtgac acccgatcac agctacacaa gaatgctgat gcactggggc
     5101 tggtttctgg aacacgatct ggaccatacc gtgcctgctc tgagcaccgc cagattttcc
     5161 gatggcagac cttgcagcag cgtgtgcacc aacgatcctc catgcttccc catgaacacc
     5221 cggcacgctg atcctagagg aacacacgcc ccttgcatgc tgtttgccag atcttctcct
     5281 gcctgtgcct ctggcagacc aagcgctaca gtggattctg tgtacgccag agagcagatc
     5341 aaccagcaga ccgcctacat cgacggcagc aatgtgtacg gcagcagcga gagagaatct
     5401 caggcactga gagatcctag cgtgcccaga ggcctgctga aaacaggatt tccttggcca
     5461 cctagcggca agcccctgct gccattttct acaggccctc caaccgagtg cgccagacaa
     5521 gagcaagaga gcccttgttt tctggccggc gatcacagag ccaatgagca tctggcactg
     5581 gccgccatgc acaccctgtg gttcagagaa cacaaccgga tggccaccga gctgagcgca
     5641 ctgaatcctc actgggaggg aaataccgtg taccaagagg ccagaaagat cgtgggagcc
     5701 gagctgcagc acattaccta ctctcactgg ctgcccaagg tgctgggcga tcctggaact
     5761 agaatgctca gaggctaccg gggctacaac cccaatgtga acgccggcat catcaacagc
     5821 tttgccaccg ccgccttcag attcggacac accctgatca accccatcct gtacagactg
     5881 aacgccacac tgggagagat cagcgagggc catctgcctt tccataaggc cctgtttagc
     5941 cccagcagaa tcatcaaaga aggtggcatc gaccccgtgc tgagaggact gtttggagtg
     6001 gctgctaagt ggcgggctcc tagctatctc ctgtctcctg agctgaccca gagactgttt
     6061 agcgccgcct attctgctgc tgtggattcc gccgccacaa tcatccagag aggcagagat
     6121 cacggcatcc ctccatacgt ggacttcaga gtgttctgca acctgacctc cgtgaagaac
     6181 ttcgaggatc tgcagaatga gatcaaggac agcgagatcc ggcagaagct gcggaagctt
     6241 tacggcagcc ctggcgatat tgatctgtgg ccagcactga tggtggaaga tctgatccct
     6301 ggcacaagag tgggccccac actgatgtgt ctgttcgtga cacagttcca gcggctgcgc
     6361 gacggcgata gattttggta cgagaaccct ggcgtgttca cccctgctca gctgacacag
     6421 ctgaaacagg cttccctgag cagagtgctg tgcgacaatg gcgatagcat ccagcaggtt
     6481 caggccgacg tgttcgtgaa ggccgagtat ccccaggact acctgaactg cagcgagatt
     6541 cccaaggtgg acctgagagt gtggcaggac tgttgcgccg attgcagaag cagaggccag
     6601 ttcagagcag tgacccaaga gtcccagaag aagagaagcg cccagtacag ctaccccgtg
     6661 gacaaggaca tggaactgag ccacctgaga agcaggcagc aggacaaaat ctacgtgggc
     6721 gaagatgccc ggaatgtgac tgtgctggct aagaccaagt tctcccagga cttcagcacc
     6781 ttcgccgccg agatccaaga aaccatcaca gccctgcggg aacagattaa caagctggaa
     6841 gccagactga ggcaggccgg ttgtacagat gttcggggag tgcctcggaa ggccgaagaa
     6901 cggtggatga aggaagattg cacccactgc atctgcgagt ccggccaagt gacctgcgtg
     6961 gtggaaattt gccctcctgc tccatgtcca tcacctgagc tggtcaaggg cacatgctgc
     7021 cccgtgtgta gagatagagg catgccaagc gacagccccg agaagcgagg cgcgccgggc
     7081 ggcgacaaga cccacacctg ccccccctgc ccagctccag aactgctggg agggccttcc
     7141 gtgttcctgt tcccccccaa gcccaaggac accctgatga tcagcaggac ccccgaggtg
     7201 acctgcgtgg tggtggccgt gtcccacgag gacccagagg tgaagttcaa ctggtacgtg
     7261 gacggcgtgg aggtgcacaa cgccaagacc aagcccagag aggagcagta caacagcacc
     7321 tacagggtgg tgtccgtgct gaccgtgctg caccaggact ggctgaacgg caaagaatac
     7381 aagtgcaaag tctccaacaa ggccctggct gccccaatcg aaaagacaat cagcaaggcc
     7441 aagggccagc cacgggagcc ccaggtgtac accctgcccc ccagccggga ggagatgacc
     7501 aagaaccagg tgtccctgac ctgtctggtg aagggcttct accccagcga tatcgccgtg
     7561 gagtgggaga gcaacggcca gcccgagaac aactacaaga ccaccccccc agtgctggac
     7621 agcgacggca gcttcttcct gtacagcaag ctgaccgtgg acaagtccag gtggcagcag
     7681 ggcaacgtgt tcagctgcag cgtgatgcac gaggccctgc acaaccacta cacccagaag
     7741 tccctgagcc tgagccccgg cggcagcggt cttaatgata ttttcgaggc tcagaagatt
     7801 gagtggcatg aacaccacca ccatcaccac taaggccggc ctgtagtcac tggcacaacg
     7861 cgttacccgg taagccaatc gggtatacac ggtcgtcata ctgcagacag ggttcttcta
     7921 ctttgcaaga tagtcttgag tagtaaaata aatagataga gaaaatcagc ctcgactgtg
     7981 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     8041 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcaca acactcaacc
     8101 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     8161 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     8221 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8281 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8341 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     8401 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8461 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     8521 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     8581 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     8641 aggaactaaa ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     8701 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     8761 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     8821 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     8881 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     8941 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     9001 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     9061 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     9121 cccgccttcc tggagacatc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     9181 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     9241 cccggtgcct gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     9301 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     9361 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     9421 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9481 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     9541 gcgtaatcat ggtcattacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9601 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9661 cttaccatct ggccccagcg ctgcgatgat accgcgagaa ccacgctcac cggctccgga
     9721 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9781 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9841 taatagtttg cgcaacgttg ttgccatcgc tacaggcatc gtggtgtcac gctcgtcgtt
     9901 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9961 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
    10021 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
    10081 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10141 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10201 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10261 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10321 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10381 gggaataagg gcgacacgga aatgttgaat actcatattc ttcctt
//
