LOCUS       sequence-303193-       10465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PXDN(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..10465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             7807..7851
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7852..7869
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8009..8026)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8015..8126
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8244..8264)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8249..8578
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8429..8564
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8491..8510
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8626..8673
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8692..9291
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8692..8711)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9188..9208
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9421..9542
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9458..9477)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9512..9531
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9595..10386,10387..10455))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10218..10237
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10456..10465,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccgt tgttgctcaa
     2761 aaacctggcg ctggctgccc tagcagatgc ctgtgtttta gaaccaccgt gcggtgcatg
     2821 catctgctgc tggaagctgt tcctgccgtg gctcctcaga cctccatcct ggacctgcgg
     2881 ttcaaccgga tcagagaaat ccagcctggc gccttcagac ggctgagaaa cctgaatacc
     2941 ctgctgctga acaacaacca gatcaagaga atccccagcg gcgccttcga ggacctggaa
     3001 aatctgaagt acctgtacct ctacaagaac gagatccaga gcatcgaccg gcaggccttc
     3061 aaaggactgg cttccctgga acagctgtac ctgcacttca atcagatcga gacactggac
     3121 cccgacagct ttcagcatct gcccaagctg gaacggctgt ttctgcacaa caacagaatc
     3181 acccacctgg tgccaggcac cttcaaccac ctggaatcca tgaagcggct gcggctggat
     3241 agcaacaccc tgcactgtga ttgcgagatc ctgtggctgg ccgacctgct gaaaacatac
     3301 gccgagtctg gaaatgccca ggccgctgcc atctgcgagt accctagaag aatccagggc
     3361 agaagcgtgg ccaccatcac acccgaggaa ctgaattgcg agaggcccag aatcacaagc
     3421 gagccccagg atgccgatgt gacaagcggc aacaccgtgt acttcacctg tagagccgag
     3481 ggcaacccca agcctgagat catctggctg cgcaacaaca acgagctgag catgaagacc
     3541 gacagcagac tgaacctgct ggacgacggc accctgatga tccagaacac ccaagagaca
     3601 gaccagggca tctaccagtg catggccaag aatgtggccg gcgaagtgaa aacccaagaa
     3661 gtgaccctga gatacttcgg cagccccgcc agacctacct ttgtgatcca gcctcagaac
     3721 accgaggtgc tcgtgggaga gtctgtgaca ctggaatgta gcgccacagg acaccctcca
     3781 cctagaatca gctggaccag aggcgataga acccctctgc ctgtggaccc cagagtgaat
     3841 atcacacctt ctggcggcct gtacatccag aacgtggtgc aaggcgatag cggcgagtat
     3901 gcctgcagcg ccaccaacaa tatcgattct gtgcacgcca ccgccttcat cattgtgcag
     3961 gccctgcctc agttcaccgt gacacctcag gatagagtgg tcatcgaggg ccagaccgtg
     4021 gattttcagt gcgaggccaa gggcaatcct cctcctgtga tcgcttggac aaaaggcggc
     4081 tcccagctgt ccgtggatag aaggcatctg gtgctgagca gcggcacact gagaattagc
     4141 ggagtggccc tgcacgatca gggccagtat gagtgtcagg ccgtgaacat catcggcagc
     4201 cagaaagtgg tggcccacct gacagtgcag cctagagtga cccctgtgtt cgccagcatt
     4261 cctagcgata ccacagtgga agtgggcgcc aatgtgcagc tgccttgttc tagtcagggc
     4321 gagcctgagc ctgccatcac atggaacaaa gacggcgtgc aagtgaccga gagcggcaag
     4381 tttcatatca gccctgaggg cttcctgacc atcaatgatg tgggacctgc cgacgccggc
     4441 agatatgagt gtgtggccag aaataccatc ggctccgcct ccgtgtctat ggtgctgtct
     4501 gtgaatgtgc ccgacgtgtc cagaaacggc gatccttttg tggccacctc tatcgtggaa
     4561 gccattgcca ccgtggaccg ggccatcaat agcaccagaa cacacctgtt cgacagccgg
     4621 cctagaagcc ctaatgacct gctggccctg ttcagatacc ccagagatcc ctataccgtg
     4681 gaacaggcca gagccggcga gatctttgag agaaccctgc agctgatcca agagcacgtg
     4741 cagcacggac tgatggtgga tctgaatggc accagctacc actacaacga cctggtgtcc
     4801 ccacagtacc tgaacctgat cgccaatctg tctggctgca cagcccacag aagagtgaac
     4861 aactgcagcg acatgtgctt ccaccagaag tacagaaccc acgacggaac ctgcaacaac
     4921 ctgcagcatc ctatgtgggg agccagcctg accgcctttg aaagactgct gaagtccgtg
     4981 tacgagaacg gcttcaacac ccctagaggc atcaaccctc accggctgta caatggacat
     5041 gccctgccta tgcctcggct ggtgtctaca acactgatcg gcaccgagac agtgacccca
     5101 gatgagcagt tcacccacat gctgatgcag tggggccagt tcctggatca cgatctggat
     5161 tctaccgtgg tggctctgag ccaggccaga ttttctgatg gccagcactg cagcaacgtg
     5221 tgcagcaatg atcctccttg cttcagcgtg atgatccctc ctaacgactc cagagctaga
     5281 agcggagccc ggtgcatgtt ttttgtgcgg tctagccctg tgtgcggcag cggaatgaca
     5341 agcctgctga tgaatagcgt gtaccccagg gaacagatca accagctgac ctcctacatc
     5401 gacgcctcca atgtgtacgg cagcacagag cacgaggccc gctctattag agatctggcc
     5461 tctcacagag gcctgctgag acagggaatt gtgcagagaa gcggcaagcc tctgctgcct
     5521 tttgccaccg gacctccaac cgagtgcatg agagatgaga acgagagccc cattccttgc
     5581 tttctggccg gggatcacag agccaatgaa cagctgggcc tgaccagcat gcacaccctg
     5641 tggttcagag agcacaatcg gatcgccaca gagctgctga aactgaaccc tcactgggac
     5701 ggcgacacca tctactacga gacaagaaag atcgtgggcg ccgagattca gcacatcacc
     5761 tatcagcact ggctgcctaa gatcctgggc gaagtgggca tgagaacact gggcgagtac
     5821 cacggctacg accctggaat caacgccggc atctttaacg ccttcgccac cgctgccttc
     5881 agattcggac acaccctggt caaccctctg ctgtaccggc tggacgagaa cttccagcct
     5941 atcgctcagg accatctgcc actgcacaag gcctttttca gccccttccg gatcgtgaac
     6001 gaaggcggaa tcgatccact gctgagaggc ctgtttggcg tggccggaaa gatgagagtt
     6061 cccagccagc tgctcaatac cgagctgacc gaaagactgt tcagcatggc ccacactgtg
     6121 gccctggatc tggccgccat taacatccag agaggcagag atcacggcat ccctccatac
     6181 cacgactacc gggtgtactg caatctgagc gccgctcaca cctttgagga tctgaagaat
     6241 gagatcaaga accccgagat cagagagaag ctgaagagac tgtacggctc taccctgaac
     6301 atcgatctgt ttcccgctct ggtggtggaa gatctggtgc ctggaagcag actgggccct
     6361 acactgatgt gtctgctgag cacccagttc aagcggctga gagatggcga ccggctttgg
     6421 tacgaaaacc ctggcgtgtt cagccctgct cagctgaccc agattaagca gactagcctg
     6481 gccagaatcc tgtgcgacaa cgccgacaac atcaccagag tgcagagcga cgtgttcaga
     6541 gtggccgagt ttcctcacgg ctatggcagc tgtgacgaga tcccaagagt ggacctgaga
     6601 gtgtggcagg actgctgcga ggattgcaga accagaggcc agttcaacgc cttcagctat
     6661 cacttccggg gcagaagaag cctggaattc agctaccaag aggacaagcc caccaagaaa
     6721 acccggccta ggaagatccc ttctgtcggc agacagggcg aacacctgag caattctacc
     6781 agcgccttct ccaccagatc cgacgccagc ggaacaaacg acttccgcga gttcgtgctc
     6841 gagatgcaga aaaccatcac cgacctgcgg acccagatca aaaagctgga aagcaggctg
     6901 tccaccaccg aatgtgtgga tgctggcgga gagtcccacg ccaacaacac caagtggaag
     6961 aaggacgcct gcaccatttg cgagtgcaag gatggccaag tgacctgctt tgtggaagcc
     7021 tgtcctcctg ctacatgcgc cgtgcctgtt aatatccctg gcgcttgttg tcccgtgtgc
     7081 ctgcagaaga gagccgaaga gaaacctggc gcgccgggcg gcgacaagac ccacacctgc
     7141 cccccctgcc cagctccaga actgctggga gggccttccg tgttcctgtt cccccccaag
     7201 cccaaggaca ccctgatgat cagcaggacc cccgaggtga cctgcgtggt ggtggccgtg
     7261 tcccacgagg acccagaggt gaagttcaac tggtacgtgg acggcgtgga ggtgcacaac
     7321 gccaagacca agcccagaga ggagcagtac aacagcacct acagggtggt gtccgtgctg
     7381 accgtgctgc accaggactg gctgaacggc aaagaataca agtgcaaagt ctccaacaag
     7441 gccctggctg ccccaatcga aaagacaatc agcaaggcca agggccagcc acgggagccc
     7501 caggtgtaca ccctgccccc cagccgggag gagatgacca agaaccaggt gtccctgacc
     7561 tgtctggtga agggcttcta ccccagcgat atcgccgtgg agtgggagag caacggccag
     7621 cccgagaaca actacaagac caccccccca gtgctggaca gcgacggcag cttcttcctg
     7681 tacagcaagc tgaccgtgga caagtccagg tggcagcagg gcaacgtgtt cagctgcagc
     7741 gtgatgcacg aggccctgca caaccactac acccagaagt ccctgagcct gagccccggc
     7801 ggcagcggtc ttaatgatat tttcgaggct cagaagattg agtggcatga acaccaccac
     7861 catcaccact aaggccggcc tgtagtcact ggcacaacgc gttacccggt aagccaatcg
     7921 ggtatacacg gtcgtcatac tgcagacagg gttcttctac tttgcaagat agtcttgagt
     7981 agtaaaataa atagatagag aaaatcagcc tcgactgtgc cttctagttg ccagccatct
     8041 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     8101 tcctaataaa atgaggaaat tgcatcacaa cactcaaccc tatctcggtc tattcttttg
     8161 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     8221 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     8281 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     8341 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8401 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8461 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
     8521 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     8581 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
     8641 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catgaccgag
     8701 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     8761 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     8821 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     8881 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     8941 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     9001 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     9061 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     9121 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacatcc
     9181 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     9241 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acacgtgcta
     9301 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     9361 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     9421 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9481 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9541 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcattacca
     9601 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9661 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagcgc
     9721 tgcgatgata ccgcgagaac cacgctcacc ggctccggat ttatcagcaa taaaccagcc
     9781 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9841 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9901 tgccatcgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9961 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10021 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10081 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10141 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10201 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10261 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10321 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10381 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10441 atgttgaata ctcatattct tcctt
//
