LOCUS       sequence-303197-       10840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-DSCAM(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..10840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             8182..8226
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8227..8244
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8384..8401)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8390..8501
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8619..8639)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8624..8953
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8804..8939
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8866..8885
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9001..9048
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9067..9666
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9067..9086)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9563..9583
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9796..9917
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9833..9852)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9887..9906
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9970..10761,10762..10830))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10593..10612
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10831..10840,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccga agatctgcac
     2761 agcagcctgt acttcgtgaa cgccagcctg caagaggtgg tgttcgccag cacaacaggc
     2821 accctggttc cttgtcctgc cgccggaatt cctcctgtga cactgagatg gtatctggcc
     2881 accggcgaag agatctacga tgtgcctggc atcagacacg tgcaccccaa tggaaccctg
     2941 cagattttcc catttcctcc aagcagcttc agcaccctga tccacgacaa cacctactac
     3001 tgcaccgccg agaatcccag cggcaagatc agatcccagg acgtgcacat caaggccgtg
     3061 ctgagagaac cctacaccgt gcgcgtggaa gatcagaaaa ccatgagagg caacgtggcc
     3121 gtgttcaagt gcatcatccc cagcagcgtg gaagcctaca tcaccgtggt gtcctgggag
     3181 aaagacaccg tgtctctggt gtccggcagc cggtttctga tcacatctac tggcgccctg
     3241 tacatcaagg atgtgcagaa cgaggacggc ctgtacaact accggtgcat cacccggcac
     3301 cggtacacag gcgaaaccag acagagcaac agcgccagac tgttcgtgtc tgaccccgcc
     3361 aatagcgccc cttctatcct ggatggcttc gaccacagaa aggccatggc tggccagaga
     3421 gtggaactgc cttgtaaagc cctgggacac cccgagcctg actacagatg gctgaaggac
     3481 aacatgcccc tggaactgag cggccggttc cagaaaacag tgaccggact gctgatcgag
     3541 aacatcagac ccagcgacag cggcagctac gtgtgcgagg tgtccaatag atacggcacc
     3601 gccaaagtga tcggccggct gtatgtgaag cagcccctga aggccacaat cagccccaga
     3661 aaagtgaagt ccagcgtggg ctctcaggtg tccctgagct gttctgtgac aggcaccgag
     3721 gaccaagagc tgagctggta cagaaacggc gagattctga accccggcaa gaacgtgcgg
     3781 atcaccggca tcaaccacga gaacctgatc atggaccaca tggtcaagag cgacggcgga
     3841 gcctaccagt gctttgtgcg gaaggataag ctgagcgccc aggactatgt gcaggtcgtg
     3901 ctggaagatg gcacccctaa gatcatcagc gccttcagcg agaaggtggt gtctcctgcc
     3961 gaacctgtgt ctctgatgtg caacgtgaag ggcacccctc tgcctaccat cacctggaca
     4021 ctggacgacg accccatcct gaaaggcggc agccacagaa tcagccagat gatcaccagc
     4081 gagggcaatg tggtgtctta cctgaacatc agcagcagcc aagtccgaga tggcggcgtg
     4141 tacagatgta ccgccaacaa ttctgccggc gtggtgctgt accaggccag aatcaatgtt
     4201 cggggccctg ccagcatcag gcccatgaag aatatcaccg ccattgccgg cagagatacc
     4261 tacatccact gccgcgtgat cggctacccc tactacagca tcaagtggta caagaacagc
     4321 aacctgctgc cgttcaacca cagacaggtg gccttcgaga acaatggcac cctgaagctg
     4381 tccgacgtgc agaaagaagt ggacgagggc gagtacacct gtaacgtgct ggttcagcca
     4441 cagctgagca ccagccagtc tgtgcacgtg acagtgaagg tgccaccttt catccagcct
     4501 ttcgagttcc ccagattcag catcggccag cgggtgttca tcccttgtgt ggtcgttagc
     4561 ggcgacctgc ctatcaccat cacatggcag aaggacggca gacccattcc tggatctctg
     4621 ggcgtgacca tcgacaacat cgacttcacc tccagcctgc ggatctccaa cctgagcctg
     4681 atgcacaacg gcaactacac atgtatcgcc cggaatgagg ccgctgccgt ggaacatcag
     4741 tctcagctga tcgtcagagt gcctcctaag ttcgtggtgc agcctagaga tcaggacggc
     4801 atctacggaa aggccgtgat cctgaattgc agcgccgagg gctatcccgt gcctacaatc
     4861 gtgtggaagt tcagcaaagg cgctggcgtg ccccagttcc agcctatcgc tctgaatggc
     4921 agaatccagg tgctgagcaa cggcagcctg ctgatcaagc acgtggtgga agaggacagc
     4981 ggctactacc tgtgtaaagt gtccaacgac gtgggcgccg acgtgtccaa gagcatgtat
     5041 ctgaccgtga agatccccgc catgatcaca agctacccca acaccacact ggccacacag
     5101 ggccagaaaa aagagatgag ctgcacagcc cacggcgaga agcccatcat cgtcagatgg
     5161 gaaaaagagg accggatcat caaccctgag atggcccgct acctggtgtc caccaaagaa
     5221 gtcggcgagg aagtcatcag cacactgcag atcctgccta ccgtgcggga agatagcggc
     5281 ttctttagct gccacgccat caacagctac ggcgaggaca gaggcatcat ccagctgaca
     5341 gtgcaagagc ctcctgatcc tcctgagatc gagatcaagg acgtgaaggc ccggaccatc
     5401 acactgaggt ggaccatggg cttcgacggc aacagcccta tcaccggcta cgacatcgag
     5461 tgcaagaaca agtccgacag ctgggacagc gcccagagga caaaggatgt gtcccctcag
     5521 ctgaactccg ccaccatcat cgatattcac cccagctcca cctactccat caggatgtac
     5581 gccaagaacc ggatcggcaa gagcgagccc agcaacgagc tgacaatcac cgctgatgag
     5641 gctgcccctg atggccctcc tcaagaagtg cacctggaac ctatcagcag ccagagcatc
     5701 agagtgacct ggaaggcccc taagaagcac ctccagaacg gcatcatcag aggctaccag
     5761 atcggctata gagagtactc caccggcggc aacttccagt tcaacatcat ctccgtggat
     5821 accagcggcg acagcgaggt gtacacactg gacaacctga acaagttcac ccagtacggc
     5881 ctggtggtgc aggcctgtaa tagagccgga acaggccctt ccagccaaga gattatcacc
     5941 accacactcg aggacgtgcc cagctatcct ccagagaacg tgcaggccat tgccacctct
     6001 ccagagagca tctccatcag ctggtctacc ctgagcaaag aggccctgaa cggaatcctg
     6061 cagggcttca gagtgatcta ctgggccaac ctgatggacg gcgagctggg agagatcaag
     6121 aacattacca ccactcagcc cagcctcgag ctggatggcc tggaaaagta caccaattac
     6181 agcattcagg tgctggcctt caccagagct ggcgacggcg ttagaagcga gcagatcttt
     6241 acccggacca aagaggacgt ccccggacct cctgctggtg tcaaagctgc agcagcctct
     6301 gcctctatgg tgtttgtgtc ctggctgcct cctctgaagc tgaacgggat catccggaag
     6361 tacaccgtgt tttgctctca cccctatcct accgtgatca gcgagttcga ggcctctcct
     6421 gacagcttct cctacagaat ccccaacctg tccagaaaca gacagtacag cgtgtgggtc
     6481 gtcgccgtga catctgccgg aagaggcaat agcagcgaga tcatcacagt ggaacccctg
     6541 gccaaggctc ccgccagaat cctgacattt tccggcaccg tgaccacacc ttggatgaag
     6601 gacatcgtgc tgccctgcaa agccgtgggc gatccatctc ctgctgtgaa gtggatgaag
     6661 gatagcaacg gcacccctag cctggtcaca atcgacggca gaagatccat cttcagcaat
     6721 ggcagcttca tcatcaggac cgtgaaagcc gaggactccg gctactatag ctgcattgcc
     6781 aacaacaact ggggctccga tgagatcatc ctgaacctgc aagtgcaggt cccacctgac
     6841 cagcctagac tgaccgtgtc taagaccacc agctccagca tcaccctgtc ttggctgcca
     6901 ggcgataatg gcggcagctc tatcaggggc tacatcctgc agtactccga ggacaactct
     6961 gagcagtggg gcagcttccc tatcagccct agcgagagaa gctaccggct cgagaatctg
     7021 aagtgcggca catggtacaa gtttaccctg acagcccaga atggcgtcgg cccaggcaga
     7081 atctccgaga tcattgaggc caagacactg ggcaaagagc ctcagttctc caaagagcaa
     7141 gagctgttcg cctccatcaa caccaccaga gtgcgcctga atctgatcgg ctggaatgac
     7201 ggcggctgtc ccatcaccag ctttaccctg gaataccggc ctttcggcac caccgtgtgg
     7261 acaactgccc agagaacaag cctgagcaag tcttacatcc tgtacgacct ccaagaggcc
     7321 acttggtacg agctgcagat gagagtgtgt aacagcgccg gctgtgccga gaagcaggcc
     7381 aatttcgcca cactgaacta cgacggcagc acaatccctc cactgatcaa gtctgtggtg
     7441 cagaatgaag agggcctgac cacaaacgag ggactgaaga tgggcgcgcc gggcggcgac
     7501 aagacccaca cctgcccccc ctgcccagct ccagaactgc tgggagggcc ttccgtgttc
     7561 ctgttccccc ccaagcccaa ggacaccctg atgatcagca ggacccccga ggtgacctgc
     7621 gtggtggtgg ccgtgtccca cgaggaccca gaggtgaagt tcaactggta cgtggacggc
     7681 gtggaggtgc acaacgccaa gaccaagccc agagaggagc agtacaacag cacctacagg
     7741 gtggtgtccg tgctgaccgt gctgcaccag gactggctga acggcaaaga atacaagtgc
     7801 aaagtctcca acaaggccct ggctgcccca atcgaaaaga caatcagcaa ggccaagggc
     7861 cagccacggg agccccaggt gtacaccctg ccccccagcc gggaggagat gaccaagaac
     7921 caggtgtccc tgacctgtct ggtgaagggc ttctacccca gcgatatcgc cgtggagtgg
     7981 gagagcaacg gccagcccga gaacaactac aagaccaccc ccccagtgct ggacagcgac
     8041 ggcagcttct tcctgtacag caagctgacc gtggacaagt ccaggtggca gcagggcaac
     8101 gtgttcagct gcagcgtgat gcacgaggcc ctgcacaacc actacaccca gaagtccctg
     8161 agcctgagcc ccggcggcag cggtcttaat gatattttcg aggctcagaa gattgagtgg
     8221 catgaacacc accaccatca ccactaaggc cggcctgtag tcactggcac aacgcgttac
     8281 ccggtaagcc aatcgggtat acacggtcgt catactgcag acagggttct tctactttgc
     8341 aagatagtct tgagtagtaa aataaataga tagagaaaat cagcctcgac tgtgccttct
     8401 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     8461 actcccactg tcctttccta ataaaatgag gaaattgcat cacaacactc aaccctatct
     8521 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     8581 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     8641 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8701 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     8761 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     8821 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8881 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8941 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     9001 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     9061 taaaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     9121 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     9181 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     9241 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     9301 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     9361 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     9421 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     9481 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     9541 ttcctggaga catccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     9601 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     9661 gcctgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     9721 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     9781 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     9841 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     9901 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     9961 tcatggtcat taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10021 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10081 atctggcccc agcgctgcga tgataccgcg agaaccacgc tcaccggctc cggatttatc
    10141 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10201 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10261 tttgcgcaac gttgttgcca tcgctacagg catcgtggtg tcacgctcgt cgtttggtat
    10321 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    10381 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    10441 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    10501 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    10561 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    10621 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    10681 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    10741 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    10801 aagggcgaca cggaaatgtt gaatactcat attcttcctt
//
