LOCUS       sequence-303198-        4734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-DSCAM(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..4734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gaagatctgc acagcagcct gtacttcgtg aacgccagcc tgcaagaggt ggtgttcgcc
       61 agcacaacag gcaccctggt tccttgtcct gccgccggaa ttcctcctgt gacactgaga
      121 tggtatctgg ccaccggcga agagatctac gatgtgcctg gcatcagaca cgtgcacccc
      181 aatggaaccc tgcagatttt cccatttcct ccaagcagct tcagcaccct gatccacgac
      241 aacacctact actgcaccgc cgagaatccc agcggcaaga tcagatccca ggacgtgcac
      301 atcaaggccg tgctgagaga accctacacc gtgcgcgtgg aagatcagaa aaccatgaga
      361 ggcaacgtgg ccgtgttcaa gtgcatcatc cccagcagcg tggaagccta catcaccgtg
      421 gtgtcctggg agaaagacac cgtgtctctg gtgtccggca gccggtttct gatcacatct
      481 actggcgccc tgtacatcaa ggatgtgcag aacgaggacg gcctgtacaa ctaccggtgc
      541 atcacccggc accggtacac aggcgaaacc agacagagca acagcgccag actgttcgtg
      601 tctgaccccg ccaatagcgc cccttctatc ctggatggct tcgaccacag aaaggccatg
      661 gctggccaga gagtggaact gccttgtaaa gccctgggac accccgagcc tgactacaga
      721 tggctgaagg acaacatgcc cctggaactg agcggccggt tccagaaaac agtgaccgga
      781 ctgctgatcg agaacatcag acccagcgac agcggcagct acgtgtgcga ggtgtccaat
      841 agatacggca ccgccaaagt gatcggccgg ctgtatgtga agcagcccct gaaggccaca
      901 atcagcccca gaaaagtgaa gtccagcgtg ggctctcagg tgtccctgag ctgttctgtg
      961 acaggcaccg aggaccaaga gctgagctgg tacagaaacg gcgagattct gaaccccggc
     1021 aagaacgtgc ggatcaccgg catcaaccac gagaacctga tcatggacca catggtcaag
     1081 agcgacggcg gagcctacca gtgctttgtg cggaaggata agctgagcgc ccaggactat
     1141 gtgcaggtcg tgctggaaga tggcacccct aagatcatca gcgccttcag cgagaaggtg
     1201 gtgtctcctg ccgaacctgt gtctctgatg tgcaacgtga agggcacccc tctgcctacc
     1261 atcacctgga cactggacga cgaccccatc ctgaaaggcg gcagccacag aatcagccag
     1321 atgatcacca gcgagggcaa tgtggtgtct tacctgaaca tcagcagcag ccaagtccga
     1381 gatggcggcg tgtacagatg taccgccaac aattctgccg gcgtggtgct gtaccaggcc
     1441 agaatcaatg ttcggggccc tgccagcatc aggcccatga agaatatcac cgccattgcc
     1501 ggcagagata cctacatcca ctgccgcgtg atcggctacc cctactacag catcaagtgg
     1561 tacaagaaca gcaacctgct gccgttcaac cacagacagg tggccttcga gaacaatggc
     1621 accctgaagc tgtccgacgt gcagaaagaa gtggacgagg gcgagtacac ctgtaacgtg
     1681 ctggttcagc cacagctgag caccagccag tctgtgcacg tgacagtgaa ggtgccacct
     1741 ttcatccagc ctttcgagtt ccccagattc agcatcggcc agcgggtgtt catcccttgt
     1801 gtggtcgtta gcggcgacct gcctatcacc atcacatggc agaaggacgg cagacccatt
     1861 cctggatctc tgggcgtgac catcgacaac atcgacttca cctccagcct gcggatctcc
     1921 aacctgagcc tgatgcacaa cggcaactac acatgtatcg cccggaatga ggccgctgcc
     1981 gtggaacatc agtctcagct gatcgtcaga gtgcctccta agttcgtggt gcagcctaga
     2041 gatcaggacg gcatctacgg aaaggccgtg atcctgaatt gcagcgccga gggctatccc
     2101 gtgcctacaa tcgtgtggaa gttcagcaaa ggcgctggcg tgccccagtt ccagcctatc
     2161 gctctgaatg gcagaatcca ggtgctgagc aacggcagcc tgctgatcaa gcacgtggtg
     2221 gaagaggaca gcggctacta cctgtgtaaa gtgtccaacg acgtgggcgc cgacgtgtcc
     2281 aagagcatgt atctgaccgt gaagatcccc gccatgatca caagctaccc caacaccaca
     2341 ctggccacac agggccagaa aaaagagatg agctgcacag cccacggcga gaagcccatc
     2401 atcgtcagat gggaaaaaga ggaccggatc atcaaccctg agatggcccg ctacctggtg
     2461 tccaccaaag aagtcggcga ggaagtcatc agcacactgc agatcctgcc taccgtgcgg
     2521 gaagatagcg gcttctttag ctgccacgcc atcaacagct acggcgagga cagaggcatc
     2581 atccagctga cagtgcaaga gcctcctgat cctcctgaga tcgagatcaa ggacgtgaag
     2641 gcccggacca tcacactgag gtggaccatg ggcttcgacg gcaacagccc tatcaccggc
     2701 tacgacatcg agtgcaagaa caagtccgac agctgggaca gcgcccagag gacaaaggat
     2761 gtgtcccctc agctgaactc cgccaccatc atcgatattc accccagctc cacctactcc
     2821 atcaggatgt acgccaagaa ccggatcggc aagagcgagc ccagcaacga gctgacaatc
     2881 accgctgatg aggctgcccc tgatggccct cctcaagaag tgcacctgga acctatcagc
     2941 agccagagca tcagagtgac ctggaaggcc cctaagaagc acctccagaa cggcatcatc
     3001 agaggctacc agatcggcta tagagagtac tccaccggcg gcaacttcca gttcaacatc
     3061 atctccgtgg ataccagcgg cgacagcgag gtgtacacac tggacaacct gaacaagttc
     3121 acccagtacg gcctggtggt gcaggcctgt aatagagccg gaacaggccc ttccagccaa
     3181 gagattatca ccaccacact cgaggacgtg cccagctatc ctccagagaa cgtgcaggcc
     3241 attgccacct ctccagagag catctccatc agctggtcta ccctgagcaa agaggccctg
     3301 aacggaatcc tgcagggctt cagagtgatc tactgggcca acctgatgga cggcgagctg
     3361 ggagagatca agaacattac caccactcag cccagcctcg agctggatgg cctggaaaag
     3421 tacaccaatt acagcattca ggtgctggcc ttcaccagag ctggcgacgg cgttagaagc
     3481 gagcagatct ttacccggac caaagaggac gtccccggac ctcctgctgg tgtcaaagct
     3541 gcagcagcct ctgcctctat ggtgtttgtg tcctggctgc ctcctctgaa gctgaacggg
     3601 atcatccgga agtacaccgt gttttgctct cacccctatc ctaccgtgat cagcgagttc
     3661 gaggcctctc ctgacagctt ctcctacaga atccccaacc tgtccagaaa cagacagtac
     3721 agcgtgtggg tcgtcgccgt gacatctgcc ggaagaggca atagcagcga gatcatcaca
     3781 gtggaacccc tggccaaggc tcccgccaga atcctgacat tttccggcac cgtgaccaca
     3841 ccttggatga aggacatcgt gctgccctgc aaagccgtgg gcgatccatc tcctgctgtg
     3901 aagtggatga aggatagcaa cggcacccct agcctggtca caatcgacgg cagaagatcc
     3961 atcttcagca atggcagctt catcatcagg accgtgaaag ccgaggactc cggctactat
     4021 agctgcattg ccaacaacaa ctggggctcc gatgagatca tcctgaacct gcaagtgcag
     4081 gtcccacctg accagcctag actgaccgtg tctaagacca ccagctccag catcaccctg
     4141 tcttggctgc caggcgataa tggcggcagc tctatcaggg gctacatcct gcagtactcc
     4201 gaggacaact ctgagcagtg gggcagcttc cctatcagcc ctagcgagag aagctaccgg
     4261 ctcgagaatc tgaagtgcgg cacatggtac aagtttaccc tgacagccca gaatggcgtc
     4321 ggcccaggca gaatctccga gatcattgag gccaagacac tgggcaaaga gcctcagttc
     4381 tccaaagagc aagagctgtt cgcctccatc aacaccacca gagtgcgcct gaatctgatc
     4441 ggctggaatg acggcggctg tcccatcacc agctttaccc tggaataccg gcctttcggc
     4501 accaccgtgt ggacaactgc ccagagaaca agcctgagca agtcttacat cctgtacgac
     4561 ctccaagagg ccacttggta cgagctgcag atgagagtgt gtaacagcgc cggctgtgcc
     4621 gagaagcagg ccaatttcgc cacactgaac tacgacggca gcacaatccc tccactgatc
     4681 aagtctgtgg tgcagaatga agagggcctg accacaaacg agggactgaa gatg
//
