LOCUS       sequence-303200-        4812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NOTCH3(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..4812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1544..1553
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2799..2808
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctcctcctt gtcttgatgg atctccttgt gccaacggcg gcagatgtac acagctgcct
       61 agcagagaag ccgcctgtct ttgtcctcct ggctgggttg gagaacggtg ccagctggaa
      121 gatccttgtc actctggacc ttgtgccggc agaggcgtgt gtcaatcttc tgtggtggcc
      181 ggcacagccc ggttcagctg tagatgtcct agaggcttca gaggccccga ctgtagcctg
      241 cctgatccat gtctcagcag cccttgtgct catggcgcca gatgttctgt gggccctgat
      301 ggcagattcc tgtgtagctg tcctccaggc taccagggca gatcctgcag atccgatgtg
      361 gatgagtgta gagtgggcga gccttgtaga cacggcggca cttgtctgaa tacccctggc
      421 agcttcagat gccagtgtcc tgccggatat acaggccctc tgtgcgaaaa tcctgccgtg
      481 ccatgtgcac cctctccatg cagaaatggc ggcacctgta gacagagcgg cgacctgaca
      541 tacgattgtg cctgtctgcc tggcttcgag ggccagaact gcgaagtgaa tgtggacgat
      601 tgccctggcc acagatgcct gaatggcgga acatgtgtgg acggcgtgaa cacctacaac
      661 tgtcagtgtc cacctgagtg gaccggccag ttctgtactg aggatgtcga cgagtgtcag
      721 ctgcagccca atgcctgtca taacggcgga acctgcttta acacactcgg cggccatagc
      781 tgcgtgtgtg tgaatggatg gacaggcgag agctgctccc agaacatcga tgattgtgcc
      841 accgccgtgt gctttcacgg cgccacatgt cacgatagag tggccagctt ttactgcgcc
      901 tgtcctatgg gcaagaccgg cctgctgtgt cacctggatg acgcctgtgt gtccaatcct
      961 tgccacgagg acgccatctg cgacacaaac cctgtgaacg gcagagccat ctgtacatgc
     1021 ccacctggct ttaccggcgg agcctgtgat caggacgtgg acgagtgttc tatcggcgcc
     1081 aatccatgcg agcacctggg cagatgtgtg aacacacagg gcagctttct gtgtcagtgc
     1141 ggcagaggat acaccggacc tagatgcgag acagatgtga acgagtgcct gagcggcccc
     1201 tgcagaaatc aggctacctg tctggaccgg atcggccagt ttacctgtat ctgcatggcc
     1261 ggcttcaccg gcacctattg cgaggtggac attgatgagt gccagagcag tccttgcgtg
     1321 aacggcggag tgtgcaagga cagagtgaat ggcttcagct gcacatgccc aagcggcttt
     1381 tctggcagca cctgtcagct ggacgtcgac gaatgtgcca gcacaccatg tagaaacggc
     1441 gctaagtgcg tggaccagcc tgatggctac gagtgtagat gtgccgaggg ctttgagggc
     1501 accctgtgcg acagaaatgt cgatgactgc agccccgatc catgccacca tggtagatgt
     1561 gtggatggaa tcgccagctt ctcctgtgct tgtgcccctg gctatacagg caccagatgc
     1621 gaatcccagg tggacgaatg cagaagccag ccttgcaggc atggcggcaa atgcctggac
     1681 ctggtggaca agtacctgtg tagatgccct tctggcacca caggcgtgaa ctgtgaagtc
     1741 aacatcgacg actgcgcctc taatccctgc accttcggcg tgtgcagaga tggcatcaac
     1801 agatacgact gcgtgtgcca gccaggcttc acaggacccc tgtgcaacgt ggaaatcaac
     1861 gagtgcgctt ctagcccatg tggcgaaggc ggatcttgtg tcgatggcga gaacggcttt
     1921 cggtgtctgt gccctcctgg atctctgcct cctctgtgtc tgcctccatc tcacccatgt
     1981 gctcacgagc catgcagcca cggcatctgt tatgatgccc caggcggatt cagatgcgtg
     2041 tgcgaacctg gatggtccgg gcctagatgt tctcagtctc tggctagaga tgcctgcgag
     2101 tctcagccat gtagagctgg cggcacatgc agctctgatg gcatgggctt tcactgcacg
     2161 tgccctccag gtgttcaggg cagacaatgc gagctgctga gcccctgtac acctaatcca
     2221 tgtgaacacg gcggaagatg cgagtctgct cctggacaac tgccagtctg ttcttgccct
     2281 caaggatggc agggaccaag atgccagcag gatgttgatg aatgcgctgg ccctgctcct
     2341 tgtggccctc acggaatctg tacaaatctg gccggctcct ttagctgtac ctgccacggt
     2401 ggctataccg gaccaagctg cgaccaggac atcaacgatt gcgacccaaa tccatgcctc
     2461 aacggcgggt cttgtcagga tggcgtgggc agcttctctt gtagctgcct gcctggattc
     2521 gctggcccca gatgtgcccg agatgtcgat gagtgcctgt ctaatccttg cggccctggc
     2581 acctgtacag accacgtggc aagcttcaca tgcacctgtc cgcctggata cggcggattt
     2641 cactgtgaac aggacctgcc agattgcagc cccagcagct gttttaatgg cgggacttgc
     2701 gtcgacgggg tcaacagctt ttcctgtctg tgcagaccag gctacactgg cgcccactgt
     2761 cagcatgaag ctgacccttg tctgagcagg ccatgtctcc atggcggcgt gtgttctgcc
     2821 gctcatccag gctttagatg cacttgcctg gaatccttca caggcccaca gtgccagaca
     2881 ctggtggatt ggtgtagcag acagccctgc caaaatggtg gcagatgcgt tcagacaggc
     2941 gcctactgtt tgtgtcctcc tggatggtct ggccggctgt gcgatatcag aagcctgcct
     3001 tgtagagagg ccgctgctca gatcggcgtt agactggaac aactgtgtca ggctggcggc
     3061 cagtgtgttg atgaggatag cagccactac tgtgtgtgcc ctgagggaag aaccggctct
     3121 cactgcgaac aagaggtgga cccatgtctg gcccagccgt gtcagcacgg tggaacatgt
     3181 agaggctaca tgggcggcta catgtgcgag tgtctgcccg gctacaacgg cgacaattgc
     3241 gaggatgatg ttgacgagtg cgccagccaa ccttgtcaac atggcggctc ttgcatcgat
     3301 ctggtggcca gatacctgtg cagttgccca ccaggcacac tcggagtgct gtgcgagatt
     3361 aacgaggacg attgtggccc cggacctcct ctggatagtg gccctagatg tctgcacaac
     3421 gggacctgtg tggatctcgt cggaggcttc cgatgcacat gtccaccggg ctatactgga
     3481 ctgagatgcg aggccgatat caatgagtgc agaagtggcg cttgtcacgc cgctcacacc
     3541 agagattgtc tgcaagatcc cggcggagga ttccgctgtc tctgtcatgc tggattcagc
     3601 ggccctaggt gtcagacagt gctgtcccct tgtgaaagcc agccatgcca acacggtggc
     3661 cagtgcagac cttctccagg acctggcgga ggcctgacct ttacatgtca ctgtgcccag
     3721 cctttctggg gcccaagatg cgaaagagtg gcccggtcct gtagagaact ccagtgtcca
     3781 gtgggagtgc cttgccagca aacacctaga ggacccagat gtgcttgccc tcctggcctc
     3841 tctggaccta gctgtagaag ctttccagga tctccaccag gcgccagcaa tgcctcttgt
     3901 gctgctgctc catgccttca tggtggaagc tgtagacctg ctcctctggc acccttcttc
     3961 agatgtgcat gcgcccaagg ctggactggg ccaagatgtg aagcaccagc tgctgcccct
     4021 gaggtgtccg aagaacctcg atgtccaaga gctgcctgtc aggccaaaag gggcgaccag
     4081 agatgcgaca gagagtgcaa ttctcccggc tgtggatggg atggcggcga ttgcagtctg
     4141 tctgtgggag atccttggag acagtgcgaa gccctgcagt gctggcggct gttcaacaac
     4201 agcagatgcg atcctgcctg cagctcccca gcctgtctgt acgacaattt cgattgccac
     4261 gcaggcggca gagagcggac ctgtaatcct gtgtacgaga agtactgcgc cgaccacttc
     4321 gccgacggaa gatgtgacca gggctgcaac accgaggaat gcggctggga tggactggat
     4381 tgtgcttccg aagttcccgc tctgcttgct agaggcgtgc tggttctgac agttctgctg
     4441 ccaccagagg aactgctgag aagcagcgcc gatttcctgc agagactgtc cgctatcctg
     4501 agaaccagcc tgcggttcag actggatgct cacggacagg ccatggtgtt cccttaccat
     4561 aggccatctc caggcagcga gcctagagcc agaagagaac tggctcctga agtgatcggc
     4621 agcgtcgtga tgctggaaat cgacaacaga ctgtgcctgc agagccccga gaacgaccac
     4681 tgtttccctg atgctcagag cgccgctgat tatctgggag ccctgtctgc tgtggaacgg
     4741 ctggactttc cctatcctct gagagatgtg cggggcgagc cactggaacc tccagaacct
     4801 tctgtgcccc tt
//
