LOCUS       sequence-303202-        4866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-SIGLEC1(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..4866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcttggggag ttagttctcc tcaggatgtg cagggcgtga agggctcttg tctgctgatc
       61 ccctgcatct tcagcttccc cgctgacgtg gaagtgcctg atggaatcac cgccatctgg
      121 tactacgact acagcggcca gagacaggtg gtgtctcact ctgccgatcc taagctggtg
      181 gaagccagat tcagaggcag aaccgagttc atgggcaacc ccgagcacag agtgtgcaac
      241 ctgctgctga aagacctgca gcctgaggac agcggcagct acaacttcag attcgagatc
      301 agcgaagtga accgttggag cgacgtgaag ggaaccctgg tcacagtgac cgaggaaccc
      361 agagtgccta caatcgcctc tcctgtggaa ctgctggaag gcaccgaggt ggacttcaac
      421 tgtagcaccc cttacgtgtg cctgcaagag caagttcggc tgcagtggca gggacaagat
      481 cctgccagaa gcgtgacctt caacagccag aagtttgagc caacaggcgt gggccacctg
      541 gaaaccctgc atatggctat gtcttggcag gaccacggca gaatcctgag atgccagctg
      601 agcgtggcca atcacagagc ccagagcgag atccatctgc aagtgaagta tgcccctaaa
      661 ggcgtgaaga ttctgctgag ccccagcggc agaaatatcc tgcctggcga gctggtcacc
      721 ctgacctgtc aagtgaatag cagctacccc gccgtgtcca gcatcaagtg gctgaaggat
      781 ggcgtgcggc tgcagacaaa aactggcgtt ctgcatctgc ctcaggccgc ttggagtgat
      841 gctggcgtgt acacctgtca ggccgagaat ggcgttggct ctctggtgtc tcctcctatc
      901 tctctgcaca tcttcatggc cgaggtccag gtttcccctg ccggacctat cctggaaaac
      961 cagaccgtga cactcgtgtg caacacccct aatgaggccc ctagcgacct gcggtacagc
     1021 tggtataaga accacgtgct gctcgaggac gcccactctc acacactgag actgcatctg
     1081 gccaccagag ccgataccgg cttctacttc tgcgaggtgc agaatgtgca cggctctgag
     1141 agatctggcc ctgtgtccgt ggtggtcaat caccctccac tgacacctgt gctgaccgcc
     1201 tttctggaaa cacaggccgg actcgtgggc atcctgcact gttctgtggt gtctgaacct
     1261 ctggccacac tggtgctgtc tcacggcgga catattctgg ccagcacaag cggcgatagc
     1321 gatcacagcc ctagattcag cggcaccagc ggacctaaca gcctgcggct ggaaatccgc
     1381 gacctggaag aaacagactc cggcgagtac aagtgcagcg ccaccaattc tctgggcaac
     1441 gccaccagca cactggactt tcatgccaat gccgccagac tgctgatttc tcctgctgcc
     1501 gaagtggtgg aaggacaggc tgtgacactg tcctgtagaa gcggactgag ccctacacct
     1561 gatgccaggt ttagctggta tctgaacggc gccctgctgc atgaaggccc tggatcttct
     1621 cttctgctgc ctgccgcttc tagcaccgat gccggctctt atcactgtag agccagagat
     1681 ggacacagcg cctctggacc atcttctcca gctgtcctga cagtgctgta ccctcctaga
     1741 cagcccacct tcaccaccag actggatttg gatgctgctg gtgctggcgc tgggagaaga
     1801 ggactgctgc tgtgtagagt ggacagcgat cctcctgcta gactgcagct gctgcacaag
     1861 gatagagtgg tggccacatc tctgcctagc ggcggaggat gttctacctg tggcggctgt
     1921 agccccagaa tgaaggttac aaaggcccct aacctgctga gggtcgagat ccacaatcct
     1981 ctgctcgaag aggaaggcct gtacctctgc gaagccagca atgccctggg caatgccagc
     2041 acctctgcca cctttaatgg acaggccacc gtgctggcta tcgcccctag tcatacactg
     2101 caagagggca cagaggccaa cctgacctgc aatgtgtcaa gagaagccgc cggatctccc
     2161 gccaacttca gctggtttag aaacggcgtg ctgtgggccc agggacctct tgaaacagtg
     2221 acactgctgc ccgtggccag aacagatgcc gcactgtatg cctgtcggat tctgacagaa
     2281 gctggcgccc agctgtctac acctgttctg ctgtctgtgc tgtatccacc tgacagacct
     2341 aagctgtctg ccctgctgga tatgggacag ggacacatgg ccctgttcat ctgcaccgtg
     2401 gatagcagac cactggctct gcttgccctg tttcatggcg aacatctgct ggccacaagc
     2461 ctgggacctc aggtgccatc tcatggcaga tttcaggcca aggccgaggc caatagcctg
     2521 aagctggaag tccgagaact cggcctgggc gattctggca gctatagatg cgaggccaca
     2581 aacgtgctgg gcagcagcaa caccagcctg ttctttcaag tccgcggagc ctgggtgcaa
     2641 gtgtctccat ctcctgaact ccaagagggc caagccgtcg tgctgtcttg tcaagtgcat
     2701 acaggcgtgc cagagggcac cagctacaga tggtatagag atggccagcc tctgcaagag
     2761 tccaccagcg ctacactgag attcgccgcc atcacactta cccaggctgg cgcatatcat
     2821 tgccaggctc aagccccagg ctctgccaca acaagtctgg ctgctcctat cagcctgcac
     2881 gtgtcatatg ccccaagaca cgtgaccctg accacactga tggatacagg acctggcaga
     2941 ctgggcctgc tcctgtgcag agttgattcc gatcctccag cacagctgcg actgctccat
     3001 ggcgatagac tggttgcctc tactctgcaa ggcgttggcg gacctgaagg cagctctcca
     3061 agactgcatg tcgccgtggc tcccaacact ctgagactcg aaattcacgg ggccatgctg
     3121 gaagatgaag gggtgtacat ttgcgaggcc tctaataccc tgggacaagc ctctgccagc
     3181 gccgactttg atgcacaggc agtgaatgtc caagtgtggc ctggcgccac agtcagagaa
     3241 ggccagctgg tcaatctgac atgcctcgtg tggacaacac accccgcaca gctgacctac
     3301 acatggtatc aggacggaca gcagagactg gacgcccata gcatccctct gcctaatgtg
     3361 actgtgcggg acgccacctc ttacagatgc ggagttggac ctcctggacg ggctcctaga
     3421 ctgagcagac ctatcactct ggacgtgctg tacgcccctc ggaacctgag actgacatac
     3481 ctgcttgagt ctcatggcgg ccaactggcc ctggtgctct gtactgttga tagcaggcct
     3541 cctgctcagc tcgctctgtc tcatgctggg agactgctgg cctcttctac cgccgcttct
     3601 gtgcctaata ctctgaggct ggaactgaga ggccctcagc ctagagatga gggcttttac
     3661 agctgcagcg ctagatctcc actcggccag gctaatacct ctctcgagct gagactggaa
     3721 ggcgtcagag tgattctggc tcctgaagca gctgttcccg aaggcgcccc tattaccgtg
     3781 acatgtgctg atcctgccgc tcacgcccct acactgtata cctggtatca caacggccgg
     3841 tggctgcaag aaggacctgc tgcctctctg agcttcctgg tggctacaag agcacacgct
     3901 ggcgcctata gctgtcaggc acaagatgcc cagggaacca gatctagtag accagccgct
     3961 ctgcaggttc tgtacgctcc acaggatgcc gtgctgagca gcttcagaga ctccagagcc
     4021 agatctatgg ccgtgatcca gtgtaccgtg gattctgagc ctcctgccga actggcactg
     4081 agccacgatg gaaaagtgct ggcaacatct agcggcgtgc acagccttgc ctctggaaca
     4141 ggacatgtgc aggtcgccag aaacgccctg agacttcagg tgcaagatgt gcctgccggc
     4201 gacgatacct atgtgtgtac agcccagaat ctgctgggct ccatcagcac aatcggcaga
     4261 ctgcaggttg agggcgccag agttgttgcc gaacctggac ttgatgtgcc agaaggcgcc
     4321 gctctgaacc tgtcttgtag actgcttgga ggccccggac ctgtgggcaa tagcacattc
     4381 gcctggttct ggaacgacag aaggctgcac gctgagcctg tgcctacact ggcctttaca
     4441 catgtggcta gagcccaggc aggcatgtat cactgtctgg ctgaactgcc tacaggcgct
     4501 gctgcttctg cccctgttat gctgagagtg ctctacccac caaagactcc caccatgatg
     4561 gtgttcgttg agcctgaagg cgggctgaga ggcatcctgg attgcagagt ggactccgag
     4621 ccactggcaa gcctgacact tcatctggga agcagactgg tggcatctag tcagcctcaa
     4681 ggcgctccag ccgaacctca cattcacgtc ctggcctctc caaacgcact gagagtggat
     4741 attgaggccc tgaggccttc cgaccagggc gagtatatct gtagcgcctc caatgtgctg
     4801 ggaagcgcct ctaccagcac atactttggc gtcagggcac tgcacagact gcaccagttt
     4861 cagcaa
//
