LOCUS       sequence-303204-        4956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NOTCH2(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..4956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttgcagtgta gagatggcta cgagccctgc gtgaacgaag ggatgtgcgt gacctatcac
       61 aacggcaccg gctactgcaa gtgccctgag ggctttctgg gcgagtactg ccagcacaga
      121 gatccctgcg agaagaacag atgccagaac ggcggaacat gtgtggccca ggctatgctg
      181 ggcaaagcca catgtagatg cgccagcggc tttaccggcg aggattgcca gtacagcacc
      241 agccatcctt gcttcgtgtc cagaccttgc ctgaatggcg gcacctgtca catgctgagc
      301 agagacacct acgagtgtac ctgccaagtg ggcttcaccg gcaaagaatg ccagtggacc
      361 gatgcctgcc tgtctcaccc ttgtgccaat ggcagcacat gcaccaccgt ggccaatcag
      421 ttcagctgca agtgtctgac cggcttcaca ggccagaaat gcgagacaga cgtgaacgag
      481 tgcgacatcc ctggacactg tcagcatggc ggaacctgcc tgaatctgcc tggcagctat
      541 cagtgtcagt gcccacaggg attcaccggc cagtactgtg atagcctgta cgtgccctgc
      601 gctccctctc cttgtgttaa cggtggcacc tgtagacaga ccggcgactt caccttcgag
      661 tgcaattgcc tgccaggctt cgagggctct acctgcgaga gaaacatcga cgactgcccc
      721 aaccacaggt gtcaaaatgg cggagtgtgt gtggacggcg tgaacaccta caactgcaga
      781 tgtcctcctc agtggacagg ccagttctgc accgaggatg tggatgagtg cctgctgcag
      841 cctaacgctt gtcagaatgg tggcacatgc gccaacagaa atggcggcta tggctgcgtg
      901 tgtgtgaatg gatggtccgg cgacgactgt agcgagaaca tcgatgattg cgccttcgcc
      961 agctgtaccc ctggcagcac ctgtatcgat agagtggcca gcttctcctg catgtgccca
     1021 gaaggcaaag ccggactgct gtgtcatctg gatgacgcct gcatcagcaa cccatgtcac
     1081 aaaggcgccc tgtgcgacac caatcctctg aatggccagt acatctgcac ctgtcctcag
     1141 ggctataagg gcgccgactg tacagaggac gtggacgaat gtgccatggc caacagcaat
     1201 ccttgcgagc acgccggcaa atgtgtgaac acagatggcg ccttccactg cgagtgcctg
     1261 aaaggatatg ccggacctag atgcgagatg gacatcaacg agtgccacag cgacccctgc
     1321 cagaatgatg ccacctgtct ggacaagatc ggcggcttca cttgcctgtg catgcctggc
     1381 tttaagggcg tgcactgcga gctggaaatc aatgaatgtc aaagcaaccc ctgcgtcaac
     1441 aacggccagt gcgtggacaa agtgaacaga ttccagtgcc tgtgtcctcc tggcttcact
     1501 ggcccagtgt gccagatcga cattgacgat tgcagcagca ccccttgtct gaacggcgcc
     1561 aagtgcatcg atcaccctaa cggctatgag tgccagtgcg ctacaggctt tacaggcgtg
     1621 ctgtgcgaag agaatatcga caactgcgac cccgatcctt gccaccacgg acagtgtcag
     1681 gacggcatcg acagctacac atgcatctgc aatcctggct acatgggagc catctgcagc
     1741 gaccagatcg atgagtgcta cagcagccca tgcctgaacg acggcagatg tatcgacctg
     1801 gtcaacggct accagtgcaa ctgtcagcct ggaaccagcg gagtgaactg cgagatcaac
     1861 ttcgacgatt gtgccagcaa tccctgcatc cacggcatct gcatggatgg catcaacaga
     1921 tacagctgtg tgtgcagccc cgggtttacc ggccagagat gcaacattga tatcgacgag
     1981 tgcgcctcca atccatgccg gaagggcgct acatgcatca atggcgtgaa cggcttccgg
     2041 tgtatctgcc ctgaaggacc tcaccatcct agctgctaca gccaagtgaa cgaatgcctg
     2101 agcaaccctt gtatccacgg aaactgtaca ggcggcctga gcggctacaa atgtctgtgt
     2161 gatgccggct gggtcggaat caactgcgag gtggacaaga atgagtgtct gtctaatccc
     2221 tgccaaaacg gtggaacttg cgacaacctc gtgaatggct acagatgtac ctgcaagaag
     2281 ggattcaagg ggtacaactg ccaagtcaac atagatgagt gcgcttctaa cccctgtctc
     2341 aaccagggca cctgtttcga cgacatcagc ggctatacct gccactgcgt gctgccttac
     2401 accggcaaga actgtcagac agtgctggcc ccttgcagcc ctaatccttg tgaaaacgcc
     2461 gccgtgtgca aagagagccc caacttcgag tcttacacat gcctgtgcgc ccctggatgg
     2521 cagggacaga gatgtaccat cgatattgat gagtgtatca gcaagccttg catgaatcac
     2581 ggcctgtgcc acaacaccca gggctcctat atgtgcgagt gtccaccagg ctttagcggc
     2641 atggactgcg aggaagatat tgacgactgt ctggctaacc catgccagaa tggcggctct
     2701 tgtatggacg gggtcaacac ttttagctgt ctgtgtctgc ctgggttcac cggggataag
     2761 tgccagaccg atatgaatga atgcctcagc gagccctgta aaaacggcgg gacctgcagc
     2821 gattacgtga actcttacac ctgtaaatgc caggccggct tcgacggggt gcactgtgaa
     2881 aacaatatca atgagtgcac cgagtccagc tgcttcaacg gcggcacttg cgtggacggc
     2941 attaacagct tttcttgtct gtgccccgtg ggctttactg gcagcttctg tctgcacgag
     3001 attaacgagt gcagctctca cccatgtctg aatgagggaa cctgtgtgga tggcctgggc
     3061 acctacagat gctcttgtcc tctgggatac acagggaaga attgccagac actggtcaac
     3121 ctgtgtagca gaagcccttg caagaacaag ggcacatgtg tgcagaagaa agccgagagc
     3181 cagtgtctct gtccttctgg atgggctggc gcctactgtg atgtgcccaa tgtgtcttgt
     3241 gatatcgccg ccagtagacg gggagtgctg gtggaacatc tgtgtcagca cagcggcgtg
     3301 tgcatcaacg ccggaaatac ccactactgc cagtgtccac tgggctatac cggctcctat
     3361 tgcgaggaac agctggacga gtgtgcaagt aacccttgtc agcacggggc cacctgttct
     3421 gacttcatcg gaggatacag atgcgagtgc gtgccaggat atcagggcgt gaactgtgaa
     3481 tacgaagttg acgagtgtca gaaccagcct tgccaaaatg gcgggacatg catcgacctc
     3541 gtgaaccact tcaagtgcag ctgccctcct ggcacaagag gcctgctctg tgaagaaaac
     3601 attgatgact gtgccagagg accccactgt ctcaatggcg gccagtgtat ggatcggatt
     3661 ggcggctact cctgtagatg tctgccagga tttgccgggg agagatgcga gggcgacatt
     3721 aacgaatgtc tcagtaaccc ctgcagcagc gagggcagcc tggattgcat ccagctgacc
     3781 aacgactacc tgtgcgtgtg cagatccgcc tttaccggaa ggcactgcga aaccttcgtg
     3841 gacgtgtgcc ctcagatgcc ttgtcttaat ggcggaactt gcgccgtggc cagcaacatg
     3901 cctgatggct ttatctgtcg ctgcccacct ggattttccg gcgctagatg tcaatcctcc
     3961 tgcggacaag tgaagtgcag aaagggcgag cagtgcgtgc acacagccag cggaccaaga
     4021 tgcttctgcc catctcctag agattgcgag agcggctgtg cttctagtcc ctgtcaacac
     4081 ggcggaagct gtcaccctca gagacagcca ccttactaca gctgtcagtg cgccccacct
     4141 tttagcggct ccagatgcga actgtacaca gcccctccaa gcacacctcc tgccacatgt
     4201 ctgtcccagt attgcgccga caaggcaaga gatggcgtgt gcgacgaggc ctgtaattct
     4261 cacgcatgtc aatgggatgg cggcgactgc agcctgacca tggaaaatcc ttgggccaac
     4321 tgcagctccc ctctgccttg ctgggactac atcaacaacc agtgcgacga actgtgcaac
     4381 accgtggaat gcctgttcga caatttcgag tgccagggca actccaagac ctgcaaatac
     4441 gacaagtact gcgccgatca cttcaaggac aaccactgcg atcagggctg taacagcgag
     4501 gaatgtggct gggatggact ggattgtgct gccgaccagc ctgaaaatct ggccgaggga
     4561 acactcgtga tcgtggtgct gatgcctcct gaacagctgc tccaggacgc cagatctttt
     4621 ctgagagcac tgggcaccct gctgcacacc aacctgagaa tcaagagaga ctcccagggc
     4681 gagctgatgg tgtaccccta ctatggcgag aagtccgccg ccatgaagaa acagcggatg
     4741 accagaagat ctctgcccgg cgagcaagag caagaggtgg ccggaagcaa ggtgttcctg
     4801 gaaatcgaca accggcagtg tgtccaggac tccgaccact gcttcaagaa cactgatgcc
     4861 gctgccgctc tgctggcctc tcatgctatt cagggcacac tgagctaccc tctggtgtct
     4921 gtggtgtccg agagcctgac acctgagaga acccaa
//
