LOCUS       sequence-303206-        5151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NOTCH1(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..5151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcaagaggac ctagatgttc tcaacctggc gagacatgcc tgaacggcgg aaagtgtgaa
       61 gccgccaatg gaaccgaagc ctgtgtgtgt ggcggagcct ttgtgggccc tagatgtcaa
      121 gaccccaatc cttgtctgag caccccttgc aagaatgccg gcacctgtca cgtggtggat
      181 agaagaggcg tggccgatta cgcctgtagc tgtgcccttg gctttagcgg ccctctgtgt
      241 ctgacacctc tggacaacgc ctgcctgaca aacccctgta gaaacggcgg cacatgcgat
      301 ctgctgaccc tgaccgagta caagtgcaga tgtcctcctg gatggtccgg caagagctgt
      361 cagcaagccg atccttgtgc cagcaatcct tgcgccaatg gcggccagtg tctgcctttt
      421 gaggccagct acatctgcca ctgtcctcca agctttcacg gccctacctg cagacaggat
      481 gtgaacgagt gtggccagaa acctggcctg tgtagacatg gcggaacctg ccacaacgaa
      541 gtgggcagct acagatgcgt gtgcagagcc actcacaccg gacctaattg cgagaggcct
      601 tacgtgccat gcagcccatc accttgtcag aacggcggga cttgtagacc taccggcgac
      661 gtgacacacg agtgtgcttg tctgcctggc ttcaccggcc agaactgcga ggaaaacatc
      721 gacgactgcc ccggcaacaa ctgcaaaaat ggcggagcat gtgtggacgg cgtgaacacc
      781 tacaactgta gatgccctcc agagtggaca ggccagtact gcacagagga tgtggatgag
      841 tgccagctga tgcccaatgc ctgccagaat ggcggcactt gccataatac ccacggcggc
      901 tacaactgcg tgtgtgtgaa tggatggacc ggcgaggact gctccgagaa catcgatgat
      961 tgtgcctccg ccgcctgttt ccacggcgcc acatgtcatg atagagtggc cagcttctac
     1021 tgcgagtgcc ctcacggaag aacaggcctg ctgtgtcacc tgaacgacgc ctgcatcagc
     1081 aatccctgca acgagggcag caactgcgac accaatcctg tgaacggcaa ggccatctgt
     1141 acatgcccta gcggctatac aggacctgcc tgtagtcagg acgtggacga atgttctctg
     1201 ggcgccaatc catgcgagca cgccggcaaa tgtatcaaca ccctgggcag cttcgagtgc
     1261 cagtgcctgc aaggatatac aggccccaga tgcgagatcg atgtgaatga atgcgtcagc
     1321 aacccttgcc agaacgatgc cacctgtctg gaccagatcg gcgagttcca gtgcatctgt
     1381 atgcctggct atgagggcgt gcactgcgaa gtgaataccg acgaatgcgc cagctctccc
     1441 tgcctgcaca atggcagatg cctggacaag atcaatgagt ttcagtgcga gtgtcccacc
     1501 ggctttacag gccacctgtg ccagtatgat gtcgacgagt gcgcctccac accttgcaaa
     1561 aacggcgcca agtgcctgga cggccccaat acctacactt gtgtgtgcac cgagggctac
     1621 accggaacac actgcgaggt ggacattgat gagtgcgacc ccgatccatg tcactacggc
     1681 agctgtaaag acggcgtggc aaccttcaca tgcctgtgca gacctggcta tactggccac
     1741 cactgcgaga ctaacatcaa cgagtgtagc agccagccat gcaggcacgg tggaacctgt
     1801 caggacagag acaatgccta cctgtgcttc tgcctgaagg gcaccaccgg gcctaactgt
     1861 gaaatcaacc tggacgactg cgcaagcagc ccttgcgatt ctggcacttg tctggataag
     1921 atcgacggct atgagtgtgc ctgcgagcct ggatacaccg gcagcatgtg caacatcaat
     1981 atcgatgaat gcgctggcaa cccatgtcac aatggcggga cctgtgaaga tggcatcaac
     2041 ggcttcacct gtcggtgccc tgagggctac catgatccta cctgtctgtc cgaagtgaac
     2101 gaatgcaata gcaacccctg cgtgcacggc gcctgcagag atagcctgaa tggctacaag
     2161 tgcgactgcg atccaggctg gtccgggacc aactgcgata tcaacaacaa cgaatgcgag
     2221 tctaaccctt gcgtcaacgg tggcacttgc aaggacatga ccagcggcta cgtgtgcacc
     2281 tgtagagagg gcttctctgg ccccaactgc cagaccaata ttaacgaatg tgcttctaac
     2341 ccctgcctga accagggcac ctgtatcgat gatgtggccg gctataagtg caactgtctg
     2401 ctgccttaca ccggggccac atgtgaagtg gttctggccc cttgtgcacc ctctccatgt
     2461 cgtaatggcg gcgaatgcag acagagcgag gactacgaga gcttcagctg tgtgtgccca
     2521 acaggctggc agggacagac atgcgaagtg gatatcaatg aatgtgtgct gagcccctgc
     2581 aggcatggcg ccagctgtca gaatactcat ggcggctata gatgccactg ccaggccggc
     2641 tacagcggca gaaattgcga aaccgatatc gacgattgcc ggcctaatcc atgccataac
     2701 ggcggctctt gcaccgacgg catcaacacc gccttttgcg attgcctgcc tggattcaga
     2761 ggcaccttct gcgaagagga cattaacgag tgtgcctccg atccttgccg gaatggcgcc
     2821 aattgcaccg attgcgtgga cagctacacc tgtacctgtc ctgccggctt ttccggcatc
     2881 cactgcgaaa acaacacccc tgactgcacc gagtccagct gcttcaatgg tggcacctgt
     2941 gtggatggga ttaactcctt tacatgtctg tgcccacctg ggtttaccgg cagctactgt
     3001 cagcacgatg tcaatgagtg tgactcccag ccttgtctgc acggcggtac atgtcaagat
     3061 ggctgcggct cctaccggtg tacatgtcct caaggctaca cagggcccaa ctgtcagaac
     3121 ctggtgcatt ggtgcgacag cagcccatgc aagaatggcg gaaagtgctg gcagacccac
     3181 acacagtata gatgcgaatg cccttctggc tggaccggcc tgtattgtga tgtgccttct
     3241 gtgtcttgcg aggtcgccgc tcaaagacag ggtgtcgatg tggctagact gtgtcaacac
     3301 ggtggactgt gcgtggacgc tggcaatacc catcattgca gatgccaggc tgggtacaca
     3361 ggcagctatt gcgaggatct ggtggacgag tgctccccat ctccatgcca aaatggcgct
     3421 acctgtaccg actacctcgg cggatacagc tgcaaatgtg tggccggata ccatggcgtg
     3481 aactgcagcg aagagattga cgagtgcctg tctcacccct gccaaaacgg tggtacatgc
     3541 ctggatctgc ccaacacata caagtgctct tgccctagag gcacccaggg tgtccattgc
     3601 gaaatcaatg ttgacgactg caaccctcct gtggaccccg tgtccagatc tcccaagtgc
     3661 ttcaacaatg ggacctgcgt ggaccaagtc ggcggctatt cttgtacttg ccctcctggc
     3721 ttcgtgggcg agagatgtga aggcgacgtc aacgaatgtt tgtctaatcc ctgtgacgcc
     3781 aggggcaccc agaattgcgt gcagagagtg aacgacttcc actgtgaatg cagagccgga
     3841 cacactggga gaagatgcga gtccgtgatc aatggctgca agggcaaacc atgtaaaaac
     3901 ggcggaactt gcgccgtggc ctctaatacc gctagaggct tcatctgcaa gtgtcccgcc
     3961 ggatttgagg gcgccacttg cgaaaatgat gccagaacct gcggcagcct gagatgcctt
     4021 aatggtggaa catgcatcag cggccctaga agccctactt gcctgtgtct gggccctttc
     4081 acaggccccg agtgtcagtt tcctgccagt tctccttgtc tcggcggaaa cccctgctac
     4141 aatcagggaa catgcgagcc taccagcgag agccccttct acagatgtct gtgtcctgcc
     4201 aagttcaacg gactgctgtg ccacatcctg gattacagct ttggaggcgg cgctggcaga
     4261 gacatccctc cacctcttat tgaggaagcc tgcgaactgc ccgagtgcca agaggatgct
     4321 ggcaacaaag tgtgcagcct gcagtgcaac aatcacgcct gtggctggga tggcggagac
     4381 tgctccctga acttcaacga cccctggaag aactgcaccc agtctctgca gtgctggaag
     4441 tactttagcg acggccactg cgacagccag tgtaattctg ccggctgcct gttcgacggc
     4501 ttcgattgtc agcgagctga gggccagtgc aatcccctgt acgaccagta ttgcaaggat
     4561 cacttctccg atggccactg tgaccagggc tgtaatagcg ccgaatgcga atgggatggc
     4621 ctggattgtg ccgagcacgt gccagaaaga ctggctgctg gaacactggt ggtggtcgtg
     4681 ctgatgcctc ctgagcagct gagaaacagc agcttccact tcctgcgcga gctgtccaga
     4741 gtgctgcaca ccaatgtggt gttcaagaga gatgcccacg gccagcagat gatcttccca
     4801 tattacggca gagaagagga actgcggaag caccctatca agagagctgc tgaaggctgg
     4861 gctgctcctg atgctctgct gggacaagtg aaagcctctc tgcttcctgg cggaagcgaa
     4921 ggcggaaggc ggagaagaga actcgatcct atggatgtgc ggggcagcat cgtgtacctg
     4981 gaaatcgaca acagacagtg cgtgcaggcc tccagccaat gttttcagtc tgctaccgac
     5041 gtggccgcct ttctgggagc acttgcttcc ctgggatctc tgaacatccc ctacaagatc
     5101 gaggccgtgc agtccgagac agtggaacct cctcctcctg ctcaactgca t
//
