LOCUS       sequence-303207-       11842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11842)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 11842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-SDK2(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..11842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             9184..9228
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             9229..9246
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9386..9403)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9392..9503
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9621..9641)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9626..9955
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9806..9941
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9868..9887
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10003..10050
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10069..10668
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10069..10088)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10565..10585
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10798..10919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10835..10854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10889..10908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(10972..11763,11764..11832))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11595..11614
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(11833..11842,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccca agatgatgtg
     2761 tccccttact ttaagaccga gcctgtgcgg acccaggtcc accttgaggg aaatagactg
     2821 gtgctgacct gcatggccga aggctcttgg cctctggaat tcaagtggct gcacaacaac
     2881 agagagctga ccaagttcag cctggaatac cggtacatga tcaccagcct ggatagaacc
     2941 cacgccggct tctacagatg catcgtgcgg aatagaatgg gcgccctgct gcagagacag
     3001 acagaggtgc aagtggccta catgggcagc ttcgaggaag gcgagaaaca ccagtctgtg
     3061 tctcatggcg aggccgccgt gatcagagca cctagaattg ccagctttcc ccagcctcaa
     3121 gtgacctggt tcagagatgg ccggaagatc cctcctagca gcagaatcgc catcacactg
     3181 gaaaacaccc tggtcatcct gagcacagtg gcccctgatg ccggcagata ttacgtgcag
     3241 gccgtgaacg acaagaacgg cgacaacaag accagccagc ctatcacact gaccgtggaa
     3301 aatgttggcg gccctgccga tcctatcgct cccacaatca tcatccctcc aaagaacaca
     3361 agcgtggtgg ccggcacaag cgaagtgaca ctggaatgcg tggccaacgc cagacctctg
     3421 atcaagctgc acatcatttg gaagaaagac ggcgtgctgc tgagcggcgg catcagcgat
     3481 cataacagac ggctgacaat ccccaatcct acaggctccg atgccggcta ctatgagtgt
     3541 gaagccgtgc tgagaagcag cagcgtgcca tctgttgtca gaggcgccta cctgtctgtg
     3601 ctggaacctc ctcagttcgt gaaagagccc gagagacaca tcaccgccga gatggaaaag
     3661 gtggtggata tcccttgtca ggccaagggc gtgccaccac ctagcatcac atggtacaag
     3721 gacgccgccg tggtggaagt ggaaaagctg accagattcc ggcagagaaa cgatggcggc
     3781 ctgcagattt ctggcctggt gcctgatgat accggcatgt tccagtgctt cgccagaaat
     3841 gcagccggcg aggtgcagac aagcacatac ctggccgtga cctctatcgc ccctaacatc
     3901 acaagaggcc ctctggacag caccgtgatc gatggaatgt ctgtggtgct ggcctgtgaa
     3961 acctctggcg cccctagacc tgccattacc tggcagaagg gcgagagaat tctggccagt
     4021 ggaagcgtgc agctgcctag attcacacct ctggaaagcg gcagcctgct gatctcccct
     4081 acacacatct ctgacgccgg cacctacacc tgtctggcca caaatagtag aggcgtggac
     4141 gaagccagcg ccgatctggt tgtttgggcc agaaccagaa tcaccaagcc tccacaggac
     4201 cagagcgtga tcaagggcac acaggcctct atggtttgcg gcgtgacaca cgaccctaga
     4261 gtgaccatcc ggtacatctg ggagaaagat ggggccacac tgggcaccga gtctcaccct
     4321 agaatcagac tggacagaaa cggctccctg cacatctctc agacttggag cggcgacatc
     4381 ggcacataca catgcagagt gatcagcgct ggcggcaacg acagtagatc tgcccacctg
     4441 agagtcagac agctgcctca cgctcctgaa caccctgtgg ctaccctgtc cacagtggaa
     4501 agaagggcca tcaacctgac ctggaccaag cctttcgacg gcaatagccc tctgatccgc
     4561 tacatcctgg aaatgagcga gaacaacgcc ccttggacag tgctgctggc ctctgtggac
     4621 cctaaggcca cctctgtgac agtgaaagga ctggtgccag ccagaagcta ccagttcaga
     4681 ctgtgcgccg tgaatgatgt cggcaagggc cagttcagca aggacaccga aagagtgtcc
     4741 ctgcctgagg aacctccaac cgctcctcct cagaatgtga tcgccagcgg caggaccaac
     4801 cagagcatca tgattcagtg gcagccacct cctgagagcc accagaatgg catcctgaag
     4861 ggctacatca tcagatactg cctggccgga ctgcccgtgg gctatcagtt caagaacatc
     4921 accgacgccg acgtgaacaa cctgctgctg gaagatctga tcatctggac caactacgag
     4981 atcgaggtgg ccgcctacaa ttctgccggc ctgggcgtgt acagcagcaa agtgacagaa
     5041 tggacactgc agggcgtccc aacagtgcct cctggcaatg tgcatgccga ggccaccaat
     5101 agcaccacca tcagattcac ctggaacgcc ccatctccac agttcatcaa cggcatcaac
     5161 cagggctaca agctgatcgc ctgggagcct gagcaagagg aagaggtcac aatggtcacc
     5221 gccaggccta acttccagga tagcatccac gtgggcttcg tgtccggcct gaagaagttc
     5281 accgagtact tcaccagcgt gctgtgcttc acaacacctg gcgacggccc tagatctaca
     5341 cctcagctcg tgcggacaca cgaggatgtt ccaggacctg tgggccacct gagcttcagc
     5401 gagattctgg ataccagcct gaaggtgtcc tggcaagagc ctggcgagaa gaacggcatt
     5461 ctgaccggct acagaatcag ctgggaagag tacaaccgga ccaacaccag agtgacccac
     5521 tacctgccta acgtgaccct cgagtacaga gtgacaggac tgacagccct gaccacctac
     5581 acaattgaag tggccgccat gaccagcaaa ggccagggac aagtgtctgc cagcacaatc
     5641 tctagcggag tgccacctga actgcccgga cctcctacaa acctgggcat cagcaatatc
     5701 ggccccagaa gcgtgaccct gcagtttaga ccaggctacg acggcaagac ctccatcagc
     5761 agatggctgg tggaagccca agtgggagtt gttggagagg gcgaagagtg gctgctgatc
     5821 caccagctga gcaatgagcc cgacgccaga tccatggaag tgcccgacct gaatcctttt
     5881 acctgctaca gcttccggat gcggcaagtg aacatcgtgg gcacatctcc acctagccag
     5941 ccaagcagaa agatccagac tctgcaggct cctcctgaca tggcccctgc taatgtgtcc
     6001 ctgagaacag ccagcgagac aagcctgtgg ctgagatgga tgcctctgcc agagatggag
     6061 tacaacggca atccagagag cgtggggtac aagatcaagt acagcagaag cgacggccac
     6121 ggaaagacac tgtctcacgt ggtgcaggac agagtggaac gggactacac catcgaggac
     6181 ctggaagagt ggaccgagta tagagtccag gtgcaggcct tcaatgccat cggatctgga
     6241 ccttggagcc agaccgttgt gggcagaaca agagaatctg tgcctagcag cggccccacc
     6301 aatgtgtctg ctctggctac cacaagcagc tccatgcttg tccgttggag cgaagtgcct
     6361 gaggccgata gaaatggact ggtcctgggc tacaaagtga tgtacaaaga gaaggacagc
     6421 gacacccagc ctcggttttg gctggtcgag ggcaattctt caagaagcgc tcagctgaca
     6481 ggcctgggca aatacgtgct gtatgaggtg caggttctgg ctttcaccag aatcggcgac
     6541 ggaagccctt ctcaccctcc aattctggaa agaaccctgg acgacgtgcc agggcctcct
     6601 atgggaatcc tgtttcctga agtgcggacc acctccgtgc gactgatttg gcaacctcct
     6661 gccgcaccta atggcatcat cctggcctac cagatcaccc acagactgaa caccaccacc
     6721 gccaatactg ccaccgtgga agttctggca cctagcgcca gacagtatac cgccacaggc
     6781 ctgaagcctg agagcgtgta cctgttcaga atcacagccc agaccagaaa aggctggggc
     6841 gaagctgctg aagccctggt ggtcaccacc gagaagagag atagacctca gcctccaagc
     6901 agacccatgg tgcaacaaga agatgtgcgg gccagatctg tgctgctgtc ttgggaacct
     6961 ggctctgatg gcctgtctcc tgtgcggtac tacacaatcc agacaagaga gctgccatcc
     7021 ggcagatggg ctctgcattc tgcctccgtg tctcacaacg cctccagctt catcgtggac
     7081 cggctgaagc cattcaccag ctacaagttt agagtgaagg ccacaaacga tatcggcgac
     7141 agcgagttca gcgaagagag cgagtctctg actaccctgc aggcagctcc tgatgaggcc
     7201 ccaacaattc tgagcgtgac accccacacc acaacaagcg tgctgatcag atggcagcct
     7261 ccagccgagg acaagattaa cggcatcctg ctgggcttca gaatccgcta ccgggaactg
     7321 ctgtacgagg gcctgagagg ctttaccctg aggggcatta acaatcctgg cgccacatgg
     7381 gccgagctga cctccatgta cagcatgaga aacctgagca ggcccagcct gacacagtac
     7441 gagctggaca acctgaacaa acatcggaga tacgagatcc ggatgtccgt gtacaatgcc
     7501 gttggcgagg gaccttctag ccctcctcaa gaggtgttcg tgggagaagc tgtgccaacc
     7561 gccgcaccaa gaaatgtggt ggttcatggc gctaccgcca ctcagctgga tgtgacatgg
     7621 gaaccacctc cactggacag ccagaatggg gacatccagg gctataagat ctacttctgg
     7681 gaagcccaga gagggaacct gaccgagaga gtgaaaaccc tgttcctggc cgagaacagc
     7741 gtgaagctga agaacctgac aggctacacc gcctatatgg tgtccgtggc tgcttttaac
     7801 gcagcaggcg acggaccaag aagcacccca acacagggac agacacagca agctgcccct
     7861 agcgctccta gctccgtgaa gtttagcgag ctgacaacaa ccagcgtcaa cgtgtcctgg
     7921 gaagcacctc agttccccaa tggcattctg gaaggctatc ggctggtgta cgagccttgt
     7981 tctcctgtgg atggcgtgtc caagatcgtg acagtggacg tgaagggcaa cagcccactg
     8041 tggctcaaag tgaaggatct ggccgagggc gtgacctaca gattcaggat cagggccaag
     8101 acattcacat acggccccga gattgaggcc aacgtgacaa caggacctgg cgaaggtgct
     8161 ccaggaccac ctggcgtgcc aatcattgtg cggtatagca gcgccattgc catccactgg
     8221 tcaagcggag atcctggcaa aggccccatc accagatacg tgatcgaggc cagacctagc
     8281 gacgaaggac tgtgggacat cctgatcaag gacatcccca aagaggtgtc cagctacacc
     8341 ttctccatgg atatcctgaa gccaggcgtg tcctacgact tcagagtgat tgctgtgaac
     8401 gactacggct tcggcacacc tagcagtcct tcacagtctg tgcccgctca gaaggccaat
     8461 cctttctacg aggaatggtg gtttggcgcg ccgggcggcg acaagaccca cacctgcccc
     8521 ccctgcccag ctccagaact gctgggaggg ccttccgtgt tcctgttccc ccccaagccc
     8581 aaggacaccc tgatgatcag caggaccccc gaggtgacct gcgtggtggt ggccgtgtcc
     8641 cacgaggacc cagaggtgaa gttcaactgg tacgtggacg gcgtggaggt gcacaacgcc
     8701 aagaccaagc ccagagagga gcagtacaac agcacctaca gggtggtgtc cgtgctgacc
     8761 gtgctgcacc aggactggct gaacggcaaa gaatacaagt gcaaagtctc caacaaggcc
     8821 ctggctgccc caatcgaaaa gacaatcagc aaggccaagg gccagccacg ggagccccag
     8881 gtgtacaccc tgccccccag ccgggaggag atgaccaaga accaggtgtc cctgacctgt
     8941 ctggtgaagg gcttctaccc cagcgatatc gccgtggagt gggagagcaa cggccagccc
     9001 gagaacaact acaagaccac ccccccagtg ctggacagcg acggcagctt cttcctgtac
     9061 agcaagctga ccgtggacaa gtccaggtgg cagcagggca acgtgttcag ctgcagcgtg
     9121 atgcacgagg ccctgcacaa ccactacacc cagaagtccc tgagcctgag ccccggcggc
     9181 agcggtctta atgatatttt cgaggctcag aagattgagt ggcatgaaca ccaccaccat
     9241 caccactaag gccggcctgt agtcactggc acaacgcgtt acccggtaag ccaatcgggt
     9301 atacacggtc gtcatactgc agacagggtt cttctacttt gcaagatagt cttgagtagt
     9361 aaaataaata gatagagaaa atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     9421 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     9481 taataaaatg aggaaattgc atcacaacac tcaaccctat ctcggtctat tcttttgatt
     9541 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     9601 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     9661 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     9721 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9781 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9841 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     9901 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9961 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
    10021 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat gaccgagtac
    10081 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
    10141 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
    10201 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
    10261 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
    10321 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
    10381 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
    10441 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
    10501 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacatccgcg
    10561 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
    10621 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaca cgtgctacga
    10681 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
    10741 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
    10801 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10861 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10921 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc attaccaatg
    10981 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    11041 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagcgctgc
    11101 gatgataccg cgagaaccac gctcaccggc tccggattta tcagcaataa accagccagc
    11161 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11221 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11281 catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11341 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11401 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11461 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11521 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11581 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11641 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11701 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11761 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11821 ttgaatactc atattcttcc tt
//
