LOCUS       sequence-303209-       11842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11842)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 11842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PTPRQ(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..11842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             9184..9228
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             9229..9246
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9386..9403)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9392..9503
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9621..9641)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9626..9955
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9806..9941
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9868..9887
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10003..10050
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10069..10668
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10069..10088)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10565..10585
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    10798..10919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10835..10854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10889..10908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(10972..11763,11764..11832))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11595..11614
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(11833..11842,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccga gagatatcaa
     2761 gtggatctgg tgcctgacag cggcttcgtg accattagag atctcggcgg aggcgagtat
     2821 caggtggacg tgtcaaatgt ggtgcccggc acaagatacg acatcaccat cagcagcatc
     2881 agcaccacct acacaagccc cgtgaccaga atcgtgacca ccaacgtgac aaagcccgga
     2941 cctcctgtgt ttctggctgg cgaaagagtg ggatctgccg gcatcctgct gagctggaat
     3001 acccctccta atcctaacgg ccggatcatc agctacatcg tgaagtacaa agaagtgtgc
     3061 ccctggatgc agaccgtgta cacacaagtg cggagcaagc ccgatagcct ggaagtgctg
     3121 ctgaccaacc tgaatcctgg cacaacctac gagatcaagg tggccgccga aaattctgcc
     3181 ggaatcggcg tgttcagcga cccctttctg ttccaaactg ccgagtctgc ccctggcaag
     3241 gtggtcaatc tgaccgtgga agcctacaac gccagcgccg tgaagctgat ctggtatctg
     3301 cctagacagc ccaacggcaa gatcaccagc ttcaagatca gcgtgaagca cgctagaagc
     3361 ggcatcgtgg tcaaggacgt gtccatcaga gtggaagata tcctgaccgg caagctgccc
     3421 gagtgcaacg agaatagcga gagcttcctg tggtccaccg cctctccatc tccaacactg
     3481 ggaagagtga cccctccaag cagaacaacc cacagcagca gcaccctgac acagaacgag
     3541 atcagcagcg tgtggaaaga gcccatcagc ttcgtcgtga cccacctgag gccttacacc
     3601 acatacctgt ttgaggtgtc cgccgtgaca accgaggccg gctacatcga ttctacaatc
     3661 gtgcggaccc ctgagtctgt gcctgaggga cctcctcaga actgcgtgac aggcaatatc
     3721 accggcaaga gcttcagcat cctgtgggac cctccaacca tcgtgaccgg aaagttcagc
     3781 tatagagtgg aactgtacgg ccccagcggc agaatcctgg ataacagcac caaggacctg
     3841 aagttcgcct tcaccaatct gacacccttc actatgtacg atgtgtatat cgccgctgag
     3901 acaagcgccg gcacaggccc taagagcaac atctctgtgt tcacccctcc agatgtgcct
     3961 ggcgccgtgt ttgatctgca gctggccgag gtggaaagca cccaagttcg gatcacctgg
     4021 aagaagccac ggcagcctaa cggcatcatc aaccagtata gagtgaaggt gctggtgccc
     4081 gagacaggca tcatcctgga aaacaccctg ctgaccggga acaacgagta catcaacgac
     4141 cccatggctc ccgagatcgt gaacatcgtg gaacccatgg tcggactgta tgagggctct
     4201 gccgagatgt ctagcgatct gcacagcctg gccacattca tctacaactc tcaccccgac
     4261 aagaacttcc ccgccagaaa tagagccgag gaccagacaa gccctgtggt caccaccaga
     4321 aaccagtaca tcaccgatat tgccgccgag cagctgagct acgtgatcag acgactggtg
     4381 cccttcaccg agcacatgat ctccgtgtcc gcctttacca tcatgggcga aggacctcca
     4441 acagtgctga gcgtgcgaac aagacagcag gtccccagct ccatcaagat catcaattac
     4501 aagaacatct ccagctcctc catcctgctc tactgggacc caccagagta ccctaatgga
     4561 aagatcaccc actacaccat ctacgccatg gaactggaca ccaaccgggc cttccagatc
     4621 acaaccatcg acaacagctt tctgatcacc ggcctgaaga agtacaccaa gtacaagatg
     4681 agagtggccg ccagcacaca cgtgggagag tctagcctga gcgaagagaa cgacatcttt
     4741 gtgcggacca gcgaggacga gcctgaaagc tctcctcagg acgtggaagt gatcgacgtg
     4801 accgccgatg agatcagact gaagtggtcc ccacctgaaa agcccaatgg catcattatc
     4861 gcctacgagg tgctgtacaa gaatatcgac accctgtaca tgaagaacac ctccaccacc
     4921 gatatcatcc tgcggaacct gcggcctcac acactgtaca atatcagcgt gcggagctac
     4981 accagattcg gccacggaaa tcaggtgtcc tctctgctgt ccgtgcggac atctgagaca
     5041 gtgccagata gcgcccctga gaacatcacc tataagaaca tcagctccgg cgagatcgag
     5101 ctgtcctttc tgcctcctag ctctcccaac gggatcatta agaagtatac catctacctg
     5161 aagcggagca acggcaacga ggaacggacc atcaatacca ccagcctgac tcagaacatc
     5221 aaggtgctca aaaagtacac gcagtatatc atcgaagtgt ccgcaagcac actgaaaggc
     5281 gagggcgtta gaagcgcccc tatcagcatt ctgaccgaag aggatgcccc tgacagccct
     5341 ccacaggatt tttccgtgaa gcagctgtcc ggcgtgaccg tgaaactgtc ttggcaacct
     5401 cctctggaac ccaacggaat tatcctgtac tacaccgtgt acgtgtggaa ccggtccagc
     5461 ctgaaaacca tcaatgtgac cgaaaccagc ctggaactga gcgacctgga ctacaacgtg
     5521 gaatactccg cctacgtgac agccagcaca agatttggcg acggcaagac cagaagcaac
     5581 atcattagct ttcagacccc tgaaggcgcc cctagcgatc ctcctaagga tgtgtactac
     5641 gccaacctga gcagctccag catcattctg ttctggaccc cacctagcaa gccaaatggg
     5701 atcatccagt actatagcgt gtactaccgg aacaccagcg gcaccttcat gcagaacttc
     5761 accctgcacg aagtgaccaa cgacttcgac aacatgaccg tgtccaccat catcgacaag
     5821 ctgaccatct tcagctacta caccttctgg ctgaccgcct ccacaagcgt cggcaatgga
     5881 aacaagagca gcgacatcat cgaggtgtac acagaccagg acatccctga gggctttgtg
     5941 ggcaacctga cctacgagtc tatcagctcc accgccatca acgtgtcatg ggtgccacca
     6001 gcacagccta atggcctggt gttctactac gtgtccctga tcctgcagca gacccctaga
     6061 catgttcggc ctccactggt cacatacgag cggagcatct acttcgataa cctggaaaag
     6121 tataccgact acatcctgaa gatcacaccc agcaccgaga agggcttcag cgacacatac
     6181 acagcccagc tgtacatcaa gactgaagag gacgtgcccg aaacaagccc tatcatcaac
     6241 accttcaaga acctgtccag caccagcgtg ctgctttctt gggacccgcc tgtgaaacct
     6301 aacggcgcca tcatctccta cgacctgaca ctgcagggcc ccaacgagaa ctacagcttc
     6361 atcaccagcg acaactacat catcctcgaa gaactgagcc cctttacgct gtactccttc
     6421 tttgccgccg ctcggacaag aaaaggcctg ggacctagct ctatcctgtt cttctacacc
     6481 gacgagagcg tgccactggc tcctccacag aatctgaccc tgatcaactg cacctccgac
     6541 ttcgtgtggc tcaaatggtc cccttctcct ctgcctggcg gaatcgtgaa ggtgtacagc
     6601 tttaagatcc acgagcacga gacagacacg atctactaca aaaacatcag cgggttcaag
     6661 accgaggcca agctcgttgg actggaacct gtgtccacct actctatcag agtgtccgct
     6721 ttcaccaaag tcggaaacgg caaccagttc tccaacgtcg tgaagttcac cacacaagag
     6781 tccgtgccag acgtggtgca gaacatgcag tgtatggcca caagctggca gagcgtgctg
     6841 gtcaaatggg accctcctaa gaaggccaac ggaatcatca cacagtacat ggtcaccgtg
     6901 gaacggaact ccaccaaggt gtcaccccag gaccacatgt ataccttcat caagctgctg
     6961 gccaacacca gctacgtgtt caaagtgcgg gcctctacat ctgctggcga aggcgacgag
     7021 tccacctgtc atgtgtctac cctgcctgaa accgtgccta gcgtgcccac caatatcgcc
     7081 ttttccgatg tgcagagcac ctccgccaca ctgacatgga tcagacctga taccatcctg
     7141 ggctacttcc agaactacaa gattaccaca cagctgcggg cccagaaatg caaagaatgg
     7201 gagtccgagg aatgcgtcga gtaccagaag atccagtacc tgtacgaggc ccacctgacc
     7261 gaggaaacag tgtacggact gaagaaattc cggtggtaca gattccaggt ggcagcctct
     7321 acaaacgccg gctacggcaa tgccagcaac tggatcagca caaagaccct gccagggcct
     7381 cctgatggcc ctcctgaaaa tgtgcatgtg gtggctacaa gccctttcag catctctatc
     7441 tcttggagcg agcccgccgt gatcacaggc ccaacatgtt acctgatcga tgtgaagtcc
     7501 gtggacaacg acgagttcaa catctccttt atcaagagca atgaggaaaa caagaccatc
     7561 gagattaagg acctcgagat cttcacccgg tacagcgtgg tcatcaccgc cttcactgga
     7621 aacatctccg ccgcctatgt ggaaggcaag tccagcgccg agatgatcgt gactacactg
     7681 gaaagcgccc caaaggaccc acctaacaat atgaccttcc agaaaatccc cgacgaagtc
     7741 acaaagttcc agctgacctt cctgccacca agccagccaa acggaaacat tcaggtgtac
     7801 caggctctgg tgtacagaga ggatgatcct accgccgtgc agatccacaa tctgtccatc
     7861 atccagaaaa cgaacacctt cgtgatcgcc atgctcgagg gactcaaagg cggccacacc
     7921 tacaacattt ccgtgtacgc cgtgaatagc gctggcgccg gacctaaggt gcccatgaga
     7981 atcaccatgg acatcaaggc ccctgccaga cctaagacca agccaacacc tatctacgac
     8041 gccaccggaa agctgctcgt gacaagcacc accatcacaa tcagaatgcc catctgctac
     8101 tacagcgacg atcacggccc catcaagaac gtgcaggtcc tggttacaga aacaggcgcc
     8161 cagcacgatg gcaacgtgac caagtggtac gacgcctact tcaacaaggc cagaccttac
     8221 ttcaccaacg agggcttccc caatcctcct tgtaccgagg gaaagaccaa gttcagcggc
     8281 aacgaagaga tctacatcat tggcgccgac aacgcctgca tgatccccgg aaatgaggac
     8341 aagatctgca acggccctct gaagcccaag aagcagtatc tgttcaagtt ccgggccacc
     8401 aacatcatgg gacagttcac cgacagcgac tactctgacc cagtgaaaac actcggcgag
     8461 ggcctgtctg agagaaccgt tgaaggcgcg ccgggcggcg acaagaccca cacctgcccc
     8521 ccctgcccag ctccagaact gctgggaggg ccttccgtgt tcctgttccc ccccaagccc
     8581 aaggacaccc tgatgatcag caggaccccc gaggtgacct gcgtggtggt ggccgtgtcc
     8641 cacgaggacc cagaggtgaa gttcaactgg tacgtggacg gcgtggaggt gcacaacgcc
     8701 aagaccaagc ccagagagga gcagtacaac agcacctaca gggtggtgtc cgtgctgacc
     8761 gtgctgcacc aggactggct gaacggcaaa gaatacaagt gcaaagtctc caacaaggcc
     8821 ctggctgccc caatcgaaaa gacaatcagc aaggccaagg gccagccacg ggagccccag
     8881 gtgtacaccc tgccccccag ccgggaggag atgaccaaga accaggtgtc cctgacctgt
     8941 ctggtgaagg gcttctaccc cagcgatatc gccgtggagt gggagagcaa cggccagccc
     9001 gagaacaact acaagaccac ccccccagtg ctggacagcg acggcagctt cttcctgtac
     9061 agcaagctga ccgtggacaa gtccaggtgg cagcagggca acgtgttcag ctgcagcgtg
     9121 atgcacgagg ccctgcacaa ccactacacc cagaagtccc tgagcctgag ccccggcggc
     9181 agcggtctta atgatatttt cgaggctcag aagattgagt ggcatgaaca ccaccaccat
     9241 caccactaag gccggcctgt agtcactggc acaacgcgtt acccggtaag ccaatcgggt
     9301 atacacggtc gtcatactgc agacagggtt cttctacttt gcaagatagt cttgagtagt
     9361 aaaataaata gatagagaaa atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     9421 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     9481 taataaaatg aggaaattgc atcacaacac tcaaccctat ctcggtctat tcttttgatt
     9541 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     9601 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     9661 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     9721 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9781 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9841 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     9901 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9961 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
    10021 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat gaccgagtac
    10081 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
    10141 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
    10201 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
    10261 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
    10321 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
    10381 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
    10441 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
    10501 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacatccgcg
    10561 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
    10621 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaca cgtgctacga
    10681 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
    10741 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
    10801 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10861 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10921 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc attaccaatg
    10981 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    11041 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagcgctgc
    11101 gatgataccg cgagaaccac gctcaccggc tccggattta tcagcaataa accagccagc
    11161 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11221 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11281 catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11341 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11401 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11461 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11521 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11581 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11641 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11701 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11761 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11821 ttgaatactc atattcttcc tt
//
