LOCUS       sequence-303210-        5736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PTPRQ(Trunc1)-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..5736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gagagatatc aagtggatct ggtgcctgac agcggcttcg tgaccattag agatctcggc
       61 ggaggcgagt atcaggtgga cgtgtcaaat gtggtgcccg gcacaagata cgacatcacc
      121 atcagcagca tcagcaccac ctacacaagc cccgtgacca gaatcgtgac caccaacgtg
      181 acaaagcccg gacctcctgt gtttctggct ggcgaaagag tgggatctgc cggcatcctg
      241 ctgagctgga atacccctcc taatcctaac ggccggatca tcagctacat cgtgaagtac
      301 aaagaagtgt gcccctggat gcagaccgtg tacacacaag tgcggagcaa gcccgatagc
      361 ctggaagtgc tgctgaccaa cctgaatcct ggcacaacct acgagatcaa ggtggccgcc
      421 gaaaattctg ccggaatcgg cgtgttcagc gacccctttc tgttccaaac tgccgagtct
      481 gcccctggca aggtggtcaa tctgaccgtg gaagcctaca acgccagcgc cgtgaagctg
      541 atctggtatc tgcctagaca gcccaacggc aagatcacca gcttcaagat cagcgtgaag
      601 cacgctagaa gcggcatcgt ggtcaaggac gtgtccatca gagtggaaga tatcctgacc
      661 ggcaagctgc ccgagtgcaa cgagaatagc gagagcttcc tgtggtccac cgcctctcca
      721 tctccaacac tgggaagagt gacccctcca agcagaacaa cccacagcag cagcaccctg
      781 acacagaacg agatcagcag cgtgtggaaa gagcccatca gcttcgtcgt gacccacctg
      841 aggccttaca ccacatacct gtttgaggtg tccgccgtga caaccgaggc cggctacatc
      901 gattctacaa tcgtgcggac ccctgagtct gtgcctgagg gacctcctca gaactgcgtg
      961 acaggcaata tcaccggcaa gagcttcagc atcctgtggg accctccaac catcgtgacc
     1021 ggaaagttca gctatagagt ggaactgtac ggccccagcg gcagaatcct ggataacagc
     1081 accaaggacc tgaagttcgc cttcaccaat ctgacaccct tcactatgta cgatgtgtat
     1141 atcgccgctg agacaagcgc cggcacaggc cctaagagca acatctctgt gttcacccct
     1201 ccagatgtgc ctggcgccgt gtttgatctg cagctggccg aggtggaaag cacccaagtt
     1261 cggatcacct ggaagaagcc acggcagcct aacggcatca tcaaccagta tagagtgaag
     1321 gtgctggtgc ccgagacagg catcatcctg gaaaacaccc tgctgaccgg gaacaacgag
     1381 tacatcaacg accccatggc tcccgagatc gtgaacatcg tggaacccat ggtcggactg
     1441 tatgagggct ctgccgagat gtctagcgat ctgcacagcc tggccacatt catctacaac
     1501 tctcaccccg acaagaactt ccccgccaga aatagagccg aggaccagac aagccctgtg
     1561 gtcaccacca gaaaccagta catcaccgat attgccgccg agcagctgag ctacgtgatc
     1621 agacgactgg tgcccttcac cgagcacatg atctccgtgt ccgcctttac catcatgggc
     1681 gaaggacctc caacagtgct gagcgtgcga acaagacagc aggtccccag ctccatcaag
     1741 atcatcaatt acaagaacat ctccagctcc tccatcctgc tctactggga cccaccagag
     1801 taccctaatg gaaagatcac ccactacacc atctacgcca tggaactgga caccaaccgg
     1861 gccttccaga tcacaaccat cgacaacagc tttctgatca ccggcctgaa gaagtacacc
     1921 aagtacaaga tgagagtggc cgccagcaca cacgtgggag agtctagcct gagcgaagag
     1981 aacgacatct ttgtgcggac cagcgaggac gagcctgaaa gctctcctca ggacgtggaa
     2041 gtgatcgacg tgaccgccga tgagatcaga ctgaagtggt ccccacctga aaagcccaat
     2101 ggcatcatta tcgcctacga ggtgctgtac aagaatatcg acaccctgta catgaagaac
     2161 acctccacca ccgatatcat cctgcggaac ctgcggcctc acacactgta caatatcagc
     2221 gtgcggagct acaccagatt cggccacgga aatcaggtgt cctctctgct gtccgtgcgg
     2281 acatctgaga cagtgccaga tagcgcccct gagaacatca cctataagaa catcagctcc
     2341 ggcgagatcg agctgtcctt tctgcctcct agctctccca acgggatcat taagaagtat
     2401 accatctacc tgaagcggag caacggcaac gaggaacgga ccatcaatac caccagcctg
     2461 actcagaaca tcaaggtgct caaaaagtac acgcagtata tcatcgaagt gtccgcaagc
     2521 acactgaaag gcgagggcgt tagaagcgcc cctatcagca ttctgaccga agaggatgcc
     2581 cctgacagcc ctccacagga tttttccgtg aagcagctgt ccggcgtgac cgtgaaactg
     2641 tcttggcaac ctcctctgga acccaacgga attatcctgt actacaccgt gtacgtgtgg
     2701 aaccggtcca gcctgaaaac catcaatgtg accgaaacca gcctggaact gagcgacctg
     2761 gactacaacg tggaatactc cgcctacgtg acagccagca caagatttgg cgacggcaag
     2821 accagaagca acatcattag ctttcagacc cctgaaggcg cccctagcga tcctcctaag
     2881 gatgtgtact acgccaacct gagcagctcc agcatcattc tgttctggac cccacctagc
     2941 aagccaaatg ggatcatcca gtactatagc gtgtactacc ggaacaccag cggcaccttc
     3001 atgcagaact tcaccctgca cgaagtgacc aacgacttcg acaacatgac cgtgtccacc
     3061 atcatcgaca agctgaccat cttcagctac tacaccttct ggctgaccgc ctccacaagc
     3121 gtcggcaatg gaaacaagag cagcgacatc atcgaggtgt acacagacca ggacatccct
     3181 gagggctttg tgggcaacct gacctacgag tctatcagct ccaccgccat caacgtgtca
     3241 tgggtgccac cagcacagcc taatggcctg gtgttctact acgtgtccct gatcctgcag
     3301 cagaccccta gacatgttcg gcctccactg gtcacatacg agcggagcat ctacttcgat
     3361 aacctggaaa agtataccga ctacatcctg aagatcacac ccagcaccga gaagggcttc
     3421 agcgacacat acacagccca gctgtacatc aagactgaag aggacgtgcc cgaaacaagc
     3481 cctatcatca acaccttcaa gaacctgtcc agcaccagcg tgctgctttc ttgggacccg
     3541 cctgtgaaac ctaacggcgc catcatctcc tacgacctga cactgcaggg ccccaacgag
     3601 aactacagct tcatcaccag cgacaactac atcatcctcg aagaactgag cccctttacg
     3661 ctgtactcct tctttgccgc cgctcggaca agaaaaggcc tgggacctag ctctatcctg
     3721 ttcttctaca ccgacgagag cgtgccactg gctcctccac agaatctgac cctgatcaac
     3781 tgcacctccg acttcgtgtg gctcaaatgg tccccttctc ctctgcctgg cggaatcgtg
     3841 aaggtgtaca gctttaagat ccacgagcac gagacagaca cgatctacta caaaaacatc
     3901 agcgggttca agaccgaggc caagctcgtt ggactggaac ctgtgtccac ctactctatc
     3961 agagtgtccg ctttcaccaa agtcggaaac ggcaaccagt tctccaacgt cgtgaagttc
     4021 accacacaag agtccgtgcc agacgtggtg cagaacatgc agtgtatggc cacaagctgg
     4081 cagagcgtgc tggtcaaatg ggaccctcct aagaaggcca acggaatcat cacacagtac
     4141 atggtcaccg tggaacggaa ctccaccaag gtgtcacccc aggaccacat gtataccttc
     4201 atcaagctgc tggccaacac cagctacgtg ttcaaagtgc gggcctctac atctgctggc
     4261 gaaggcgacg agtccacctg tcatgtgtct accctgcctg aaaccgtgcc tagcgtgccc
     4321 accaatatcg ccttttccga tgtgcagagc acctccgcca cactgacatg gatcagacct
     4381 gataccatcc tgggctactt ccagaactac aagattacca cacagctgcg ggcccagaaa
     4441 tgcaaagaat gggagtccga ggaatgcgtc gagtaccaga agatccagta cctgtacgag
     4501 gcccacctga ccgaggaaac agtgtacgga ctgaagaaat tccggtggta cagattccag
     4561 gtggcagcct ctacaaacgc cggctacggc aatgccagca actggatcag cacaaagacc
     4621 ctgccagggc ctcctgatgg ccctcctgaa aatgtgcatg tggtggctac aagccctttc
     4681 agcatctcta tctcttggag cgagcccgcc gtgatcacag gcccaacatg ttacctgatc
     4741 gatgtgaagt ccgtggacaa cgacgagttc aacatctcct ttatcaagag caatgaggaa
     4801 aacaagacca tcgagattaa ggacctcgag atcttcaccc ggtacagcgt ggtcatcacc
     4861 gccttcactg gaaacatctc cgccgcctat gtggaaggca agtccagcgc cgagatgatc
     4921 gtgactacac tggaaagcgc cccaaaggac ccacctaaca atatgacctt ccagaaaatc
     4981 cccgacgaag tcacaaagtt ccagctgacc ttcctgccac caagccagcc aaacggaaac
     5041 attcaggtgt accaggctct ggtgtacaga gaggatgatc ctaccgccgt gcagatccac
     5101 aatctgtcca tcatccagaa aacgaacacc ttcgtgatcg ccatgctcga gggactcaaa
     5161 ggcggccaca cctacaacat ttccgtgtac gccgtgaata gcgctggcgc cggacctaag
     5221 gtgcccatga gaatcaccat ggacatcaag gcccctgcca gacctaagac caagccaaca
     5281 cctatctacg acgccaccgg aaagctgctc gtgacaagca ccaccatcac aatcagaatg
     5341 cccatctgct actacagcga cgatcacggc cccatcaaga acgtgcaggt cctggttaca
     5401 gaaacaggcg cccagcacga tggcaacgtg accaagtggt acgacgccta cttcaacaag
     5461 gccagacctt acttcaccaa cgagggcttc cccaatcctc cttgtaccga gggaaagacc
     5521 aagttcagcg gcaacgaaga gatctacatc attggcgccg acaacgcctg catgatcccc
     5581 ggaaatgagg acaagatctg caacggccct ctgaagccca agaagcagta tctgttcaag
     5641 ttccgggcca ccaacatcat gggacagttc accgacagcg actactctga cccagtgaaa
     5701 acactcggcg agggcctgtc tgagagaacc gttgaa
//
