LOCUS       sequence-30353-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Tomishige M, Vale RD
  TITLE     Controlling kinesin by reversible disulfide cross-linking. 
            Identifying the motility-producing conformational change.
  JOURNAL   J Cell Biol. 2000 Nov 27. 151(5):1081-92.
  PUBMED    11086009
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2000 Nov 27. 151(5):1081-92."
COMMENT     Sequence Label: RP hk339-GFP
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(90..110)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctttgttag cagccggatc tgctcgagtt aatggtgatg atggtgatgt ttgtatagtt
       61 catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc
      121 tcttttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta
      181 aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc
      241 ttccatcttc aatgttgtgt ctaattttga agttaacttt gattccattc ttttgtttgt
      301 ctgccatgat gtatacattg tgtgagttat agttgtattc caatttgtgt ccaagaatgt
      361 ttccatcttc tttaaaatca atacctttta actcgattct attaacaagg gtatcacctt
      421 caaacttgac ttcagcacgt gtcttgtagt tcccgtcatc tttgaaaaat atagttcttt
      481 cctgtacata accttcgggc atggcactct tgaaaaagtc atgctgtttc atatgatctg
      541 ggtatcttga aaagcattga acaccataag tcagagtagt gacaagtgtt ggccatggaa
      601 caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat gttgcatcac
      661 cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatc
//
