LOCUS       sequence-303560-         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-BTNL8-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             328..339
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 caatggcaag tgtttggacc tgataagcct gtgcaggccc tcgtgggaga agatgccgcc
       61 tttagctgct ttctgagccc caagacaaac gccgaagcca tggaagtgcg gttcttcaga
      121 ggccagttta gcagcgtggt gcacctgtac agagatggca aggaccagcc tttcatgcag
      181 atgccccagt accagggcag aacaaagctg gtcaaggact ctatcgccga gggcagaatc
      241 agcctgcggc tggaaaacat cacagtgctg gatgccggcc tgtacggctg tagaatcagc
      301 agccagagct actaccagaa ggccatctgg gagctgcaag tgtctgctct gggaagcgtg
      361 ccactgatca gcatcacagg ctacgtggac agagacatcc agctgctgtg tcagagcagc
      421 ggctggtttc ctagacctac cgccaagtgg aagggccctc agggccaaga tctgagcacc
      481 gacagccgga ccaatagaga catgcacggc ctgttcgacg tggaaatctc cctgaccgtg
      541 caagagaatg ccggcagcat cagctgtagc atgagacacg cccacctgag cagagaagtg
      601 gaaagcagag tgcagatcgg cgacaccttc ttcgagccta ttagctggca cctggccacc
      661 aaa
//
