LOCUS       sequence-30360-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Tomishige M, Vale RD
  TITLE     Controlling kinesin by reversible disulfide cross-linking. 
            Identifying the motility-producing conformational change.
  JOURNAL   J Cell Biol. 2000 Nov 27. 151(5):1081-92.
  PUBMED    11086009
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2000 Nov 27. 151(5):1081-92."
COMMENT     Sequence Label: Y344C
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..47)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(48..761)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(48..761)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(88..108)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     703..731
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcttgtagca gccggatctg ctcgagttaa tggtgatgat ggtgatgttt gtatagttca
       61 tccatgccat gtgtaatccc agcagctgtt acaaactcaa gaaggaccat gtggtctctc
      121 ttttcgttgg gatctttcga aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa
      181 aggacagggc catcgccaat tggagtattt tgttgataat ggtctgctag ttgaacgctt
      241 ccatcttcaa tgttgtgtct aattttgaag ttaactttga ttccattctt ttgtttgtct
      301 gccatgatgt atacattgtg tgagttatag ttgtattcca atttgtgtcc aagaatgttt
      361 ccatcttctt taaaatcaat accttttaac tcgattctat taacaagggt atcaccttca
      421 aacttgactt cagcacgtgt cttgtagttc ccgtcatctt tgaaaaatat agttctttcc
      481 tgtacataac cttcgggcat ggcactcttg aaaaagtcat gctgtttcat atgatctggg
      541 tatcttgaaa agcattgaac accataagtc agagtagtga caagtgttgg ccatggaaca
      601 ggtagttttc cagtagtgca aataaattta agggtaagtt ttccgtatgt tgcatcacct
      661 tcaccctctc cactgacaga aaatttgtgc ccattaacat caccatctaa ttcaacaaga
      721 attgggacaa ctccagtgaa aagttcttct cctttactca tggtaccttt tagtaaaaga
      781 tgccatcatc tcagctgctc gttttttctg gtggttggtc atttccttaa gtttctgaag
      841 ctcagcacct atactcgcta aagttgccga a
//
