LOCUS       sequence-30361-e         582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Tomishige M, Vale RD
  TITLE     Controlling kinesin by reversible disulfide cross-linking. 
            Identifying the motility-producing conformational change.
  JOURNAL   J Cell Biol. 2000 Nov 27. 151(5):1081-92.
  PUBMED    11086009
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2000 Nov 27. 151(5):1081-92."
COMMENT     Sequence Label: Y344C
FEATURES             Location/Qualifiers
     source          1..582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..58)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(99..119)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttctttcggg ctttgttagc agccggatct gctcgagtta atggtgatga tggtgatgtt
       61 tgtatagttc atccatgcca tgtgtaatcc cagcagctgt tacaaactca agaaggacca
      121 tgtggtctct cttttcgttg ggatctttcg aaagggcaga ttgtgtggac aggtaatggt
      181 tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt ttgttgataa tggtctgcta
      241 gttgaacgct tccatcttca atgttgtgtc taattttgaa gttaactttg attccattct
      301 tttgtttgtc tgccatgatg tatacattgt gtgagttata gttgtattcc aatttgtgtc
      361 caagaatgtt tccatcttct ttaaaatcaa taccttttaa ctcgattcta ttaacaaggg
      421 tatcaccttc aaacttgact tcagcacgtg tcttgtagtt cccgtcatct ttgaaaaata
      481 tagttctttc ctgtacataa ccttcgggca tggcactctt gaaaaagtca tgctgtttca
      541 tatgatctgg gtatcttgaa aagcattgaa caccataagt ca
//
