LOCUS       sequence-30364-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Tomishige M, Vale RD
  TITLE     Controlling kinesin by reversible disulfide cross-linking. 
            Identifying the motility-producing conformational change.
  JOURNAL   J Cell Biol. 2000 Nov 27. 151(5):1081-92.
  PUBMED    11086009
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2000 Nov 27. 151(5):1081-92."
COMMENT     Sequence Label: A337C/Y344C
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(47..760)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(47..760)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(87..107)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     702..730
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tggttagcag ccggatctgc tcgagttaat ggtgatgatg gtgatgtttg tatagttcat
       61 ccatgccatg tgtaatccca gcagctgtta caaactcaag aaggaccatg tggtctctct
      121 tttcgttggg atctttcgaa agggcagatt gtgtggacag gtaatggttg tctggtaaaa
      181 ggacagggcc atcgccaatt ggagtatttt gttgataatg gtctgctagt tgaacgcttc
      241 catcttcaat gttgtgtcta attttgaagt taactttgat tccattcttt tgtttgtctg
      301 ccatgatgta tacattgtgt gagttatagt tgtattccaa tttgtgtcca agaatgtttc
      361 catcttcttt aaaatcaata ccttttaact cgattctatt aacaagggta tcaccttcaa
      421 acttgacttc agcacgtgtc ttgtagttcc cgtcatcttt gaaaaatata gttctttcct
      481 gtacataacc ttcgggcatg gcactcttga aaaagtcatg ctgtttcata tgatctgggt
      541 atcttgaaaa gcattgaaca ccataagtca gagtagtgac aagtgttggc catggaacag
      601 gtagttttcc agtagtgcaa ataaatttaa gggtaagttt tccgtatgtt gcatcacctt
      661 caccctctcc actgacagaa aatttgtgcc cattaacatc accatctaat tcaacaagaa
      721 ttgggacaac tccagtgaaa agttcttctc ctttactcat ggt
//
