LOCUS       sequence-30372-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Tomishige M, Vale RD
  TITLE     Controlling kinesin by reversible disulfide cross-linking. 
            Identifying the motility-producing conformational change.
  JOURNAL   J Cell Biol. 2000 Nov 27. 151(5):1081-92.
  PUBMED    11086009
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2000 Nov 27. 151(5):1081-92."
COMMENT     Sequence Label: L4C/S330C
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(53..766)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(53..766)
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(93..113)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     708..736
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgggcttgtt agcagccgga atctgctcga gttaatggtg atgatggtga tgtttgtata
       61 gttcatccat gccatgtgta atcccagcag ctgttacaaa ctcaagaagg accatgtggt
      121 ctctcttttc gttgggatct ttcgaaaggg cagattgtgt ggacaggtaa tggttgtctg
      181 gtaaaaggac agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa
      241 cgcttccatc ttcaatgttg tgtctaattt tgaagttaac tttgattcca ttcttttgtt
      301 tgtctgccat gatgtataca ttgtgtgagt tatagttgta ttccaatttg tgtccaagaa
      361 tgtttccatc ttctttaaaa tcaatacctt ttaactcgat tctattaaca agggtatcac
      421 cttcaaactt gacttcagca cgtgtcttgt agttcccgtc atctttgaaa aatatagttc
      481 tttcctgtac ataaccttcg ggcatggcac tcttgaaaaa gtcatgctgt ttcatatgat
      541 ctgggtatct tgaaaagcat tgaacaccat aagtcagagt agtgacaagt gttggccatg
      601 gaacaggtag ttttccagta gtgcaaataa atttaagggt aagttttccg tatgttgcat
      661 caccttcacc ctctccactg acagaaaatt tgtgcccatt aacatcacca tctaattcaa
      721 caagaattgg gacaactcca gtgaaaagtt cttctccttt actcatggta ccttttagta
      781 aagatgccat catctcagct gctcgttttt tctggtgg
//
