LOCUS       sequence-30376-e         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Tomishige M, Vale RD
  TITLE     Controlling kinesin by reversible disulfide cross-linking. 
            Identifying the motility-producing conformational change.
  JOURNAL   J Cell Biol. 2000 Nov 27. 151(5):1081-92.
  PUBMED    11086009
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2000 Nov 27. 151(5):1081-92."
COMMENT     Sequence Label: L4C
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..54)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(95..115)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     710..738
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctgctc gagttaatgg tgatgatggt gatgtttgta
       61 tagttcatcc atgccatgtg taatcccagc agctgttaca aactcaagaa ggaccatgtg
      121 gtctctcttt tcgttgggat ctttcgaaag ggcagattgt gtggacaggt aatggttgtc
      181 tggtaaaagg acagggccat cgccaattgg agtattttgt tgataatggt ctgctagttg
      241 aacgcttcca tcttcaatgt tgtgtctaat tttgaagtta actttgattc cattcttttg
      301 tttgtctgcc atgatgtata cattgtgtga gttatagttg tattccaatt tgtgtccaag
      361 aatgtttcca tcttctttaa aatcaatacc ttttaactcg attctattaa caagggtatc
      421 accttcaaac ttgacttcag cacgtgtctt gtagttcccg tcatctttga aaaatatagt
      481 tctttcctgt acataacctt cgggcatggc actcttgaaa aagtcatgct gtttcatatg
      541 atctgggtat cttgaaaagc attgaacacc ataagtcaga gtagtgacaa gtgttggcca
      601 tggaacaggt agttttccag tagtgcaaat aaatttaagg gtaagttttc cgtatgttgc
      661 atcaccttca ccctctccac tgacagaaaa tttgtgccca ttaacatcac catctaattc
      721 aacaagaatt gggacaactc c
//
