LOCUS       sequence-304123-        9175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9175)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PTK7-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     RBS             4746..4754
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4780..4815
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             4825..4848
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6508..6552
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6553..6579
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(6719..6736)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6725..6836
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6954..6974)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6959..7288
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7139..7274
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7201..7220
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7336..7383
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7402..8001
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7402..7421)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7898..7918
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8131..8252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8168..8187)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8222..8241
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8305..9096,9097..9165))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8928..8947
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9166..9175,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccgc catcgtgttt
     2761 atcaagcagc ctagcagcca ggacgccctg caaggccgta gagcactgct gagatgcgaa
     2821 gtggaagccc ctggacctgt gcacgtttac tggttgcttg atggcgcccc tgtgcaggac
     2881 accgagagaa gatttgccca gggcagcagc ctgtctttcg ccgctgttga cagactgcag
     2941 gacagcggca cctttcagtg cgtggccaga gatgatgtga ccggcgagga agccagaagc
     3001 gccaacgcca gcttcaacat caagtggatc gaggctggcc ccgtggtgct gaaacatcct
     3061 gcctctgagg ccgagatcca gccacagaca caagtgaccc tgcggtgcca catcgacgga
     3121 caccctagac caacctacca gtggttcaga gatggcaccc ctctgtccga cggccagagc
     3181 aatcataccg tgtccagcaa agagcggaac ctgacactga ggcctgccgg acctgaacac
     3241 agcggcctgt attcttgttg tgcccacagc gcctttggcc aggcctgtag ctctcagaac
     3301 ttcaccctga gcattgccga cgagagcttc gccagagttg tgctggctcc tcaggatgtg
     3361 gtggtggcca gatacgaaga ggccatgttc cactgccagt tcagcgctca gcctcctcca
     3421 tctctgcagt ggctgttcga ggacgagaca cccatcacca acagaagccg gcctccacat
     3481 ctgagaaggg ccaccgtgtt tgccaatggc agcctgctgc tgactcaagt gcggcctaga
     3541 aacgccggca tctacagatg tatcggccag ggccaaagag gccctcctat catcctggaa
     3601 gccacactgc acctggccga gattgaggac atgcccctgt tcgagcccag agtgtttaca
     3661 gccggcagcg aggaaagagt gacctgcctg cctcctaagg gactgcctga gccttctgtt
     3721 tggtgggaac acgctggcgt cagactgcct acacacggca gagtgtacca gaaaggccac
     3781 gaactggtgc tggccaatat cgccgaatcc gacgcaggcg tgtacacatg tcatgccgcc
     3841 aatctggccg gacagagaag gcaggacgtg aacatcacag tggccacagt gcccagctgg
     3901 ctgaagaagc ctcaggactc tcagctggaa gagggcaagc ctggctacct ggattgtctg
     3961 acacaggcca cacctaagcc taccgtcgtg tggtacagaa accagatgct gatctccgag
     4021 gacagcagat tcgaggtgtt caagaacggc accctgcgga tcaacagcgt ggaagtgtac
     4081 gacggcacct ggtacagatg catgagcagc acaccagccg gctccattga agcccaggct
     4141 agagtgcagg tcctggaaaa gctgaagttc acccctccac ctcagccaca gcagtgcatg
     4201 gaattcgaca aagaggctac agtcccctgc agcgccacag gcagagagaa gcctacaatc
     4261 aagtgggaga gagccgacgg cagctccctg cctgaatggg tcacagataa tgccggcact
     4321 ctgcactttg ccagagtgac cagggacgac gccggcaatt atacctgtat cgccagcaat
     4381 ggcccacagg gccagattag agcccacgtg cagctgacag tggccgtgtt catcaccttc
     4441 aaggtggaac ccgagcggac caccgtgtat cagggacata cagctctgct gcagtgcgag
     4501 gctcagggcg atcctaagcc tctgatccag tggaagggca aagaccggat tctggacccc
     4561 acaaagctgg gccccagaat gcacatcttc cagaacggct ccctcgtgat ccacgatgtg
     4621 gcccctgaag atagcggccg gtacacctgt attgccggaa acagctgcaa tatcaagcac
     4681 acagaggccc ctctgtacgt ggtggacaag cctgtgcctg aggaatctga aggccctggc
     4741 agcccacctc cttacaagat gatccagaca ggcgcgccgg aagatcaagt tgatccaaga
     4801 ttgattgatg gcaagggcgg atctctggag gtgctgttcc agggaccagg cggaagcggc
     4861 aatcccggcg gcgatctggc cccccagatg ctgcgcgagc tgcaggagac caatgccgcc
     4921 ctgcaggacg tgcgcgagct gctgcgccac cgcgtgaagg agatcacctt cctgaagaac
     4981 accgtgatgg agtgcgacgc ctgcggtatg cagccctcta gaggctccgg ctccatcatc
     5041 ccagttgagg aggagaaccc ggacttctgg aaccgcgagg cagccgaggc cctgggtgcc
     5101 gccaagaagc tgcagcctgc acagacagcc gccaagaacc tcatcatctt cctgggcgat
     5161 gggatggggg tgtctacggt gacagctgcc aggatcctaa aagggcagaa gaaggacaaa
     5221 ctggggcctg agatacccct ggccatggac cgcttcccat atgtggctct gtccaagaca
     5281 tacaatgtag acaaacatgt gccagacagt ggagccacag ccacggccta cctgtgcggg
     5341 gtcaagggca acttccagac cattggcttg agtgcagccg cccgctttaa ccagtgcaac
     5401 acgacacgcg gcaacgaggt catctccgtg atgaatcggg ccaagaaagc agggaagtca
     5461 gtgggagtgg taaccaccac acgagtgcag cacgcctcgc cagccggcac ctacgcccac
     5521 acggtgaacc gcaactggta ctcggacgcc gacgtgcctg cctcggcccg ccaggagggg
     5581 tgccaggaca tcgctacgca gctcatctcc aacatggaca ttgacgtgat cctaggtgga
     5641 ggccgaaagt acatgtttcg catgggaacc ccagaccctg agtacccaga tgactacagc
     5701 caaggtggga ccaggctgga cgggaagaat ctggtgcagg aatggctggc gaagcgccag
     5761 ggtgcccggt atgtgtggaa ccgcactgag ctcatgcagg cttccctgga cccgtctgtg
     5821 acccatctca tgggtctctt tgagcctgga gacatgaaat acgagatcca ccgagactcc
     5881 acactggacc cctccctgat ggagatgaca gaggctgccc tgcgcctgct gagcaggaac
     5941 ccccgcggct tcttcctctt cgtggagggt ggtcgcatcg accatggtca tcatgaaagc
     6001 agggcttacc gggcactgac tgagacgatc atgttcgacg acgccattga gagggcgggc
     6061 cagctcacca gcgaggagga cacgctgagc ctcgtcactg ccgaccactc ccacgtcttc
     6121 tccttcggag gctaccccct gcgagggagc tccatcttcg ggctggcccc tggcaaggcc
     6181 cgggacagga aggcctacac ggtcctccta tacggaaacg gtccaggcta tgtgctcaag
     6241 gacggcgccc ggccggatgt taccgagagc gagagcggga gccccgagta tcggcagcag
     6301 tcagcagtgc ccctggacga agagacccac gcaggcgagg acgtggcggt gttcgcgcgc
     6361 ggcccgcagg cgcacctggt tcacggcgtg caggagcaga ccttcatagc gcacgtcatg
     6421 gccttcgccg cctgcctgga gccctacacc gcctgcgacc tggcgccccc cgccggcacc
     6481 accgacgccg cgcacccggg tggcggagga ctgaacgaca tcttcgaggc tcagaaaatc
     6541 gaatggcacg aacatcacca tcaccatcac catcaccatt aaggccggcc tgtagtcact
     6601 ggcacaacgc gttacccggt aagccaatcg ggtatacacg gtcgtcatac tgcagacagg
     6661 gttcttctac tttgcaagat agtcttgagt agtaaaataa atagatagag aaaatcagcc
     6721 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     6781 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcacaa
     6841 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6901 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     6961 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7021 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     7081 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7141 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7201 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     7261 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     7321 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     7381 gacaaggtga ggaactaaac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     7441 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     7501 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     7561 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     7621 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     7681 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     7741 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     7801 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     7861 gccggggtgc ccgccttcct ggagacatcc gcgccccgca acctcccctt ctacgagcgg
     7921 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     7981 acccgcaagc ccggtgcctg acacgtgcta cgagatttcg attccaccgc cgccttctat
     8041 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8101 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     8161 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8221 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     8281 tagagcttgg cgtaatcatg gtcattacca atgcttaatc agtgaggcac ctatctcagc
     8341 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8401 acgggagggc ttaccatctg gccccagcgc tgcgatgata ccgcgagaac cacgctcacc
     8461 ggctccggat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8521 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8581 ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct acaggcatcg tggtgtcacg
     8641 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8701 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8761 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8821 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8881 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8941 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9001 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9061 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9121 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatattct tcctt
//
