LOCUS       sequence-30414-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Case RB, Pierce DW, Hom-Booher N, Hart CL, Vale RD
  TITLE     The directional preference of kinesin motors is specified by an 
            element outside of the motor catalytic domain.
  JOURNAL   Cell. 1997 Sep 5. 90(5):959-66.
  PUBMED    9298907
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1997 
            Sep 5. 90(5):959-66."
COMMENT     Sequence Label: NCD-GFP
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(91..119)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gatattttgt ttactttaga aggagatata catatggcta gccatcacca tcatcaccat
       61 atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      121 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      181 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      241 gtcactactt tcacttatgg tgttcaatgc ttttcaagat acccagatca tatgaaacgg
      301 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      361 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      421 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      481 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      541 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      601 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      661 ctgtccacac aatctgccct ttcgaaagat cccancgaaa gagagacaca tggtcctctt
      721 gagttgtaca gctgctggga taccatggca tgatgactat acaaggac
//
