LOCUS       sequence-30416-e         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Case RB, Pierce DW, Hom-Booher N, Hart CL, Vale RD
  TITLE     The directional preference of kinesin motors is specified by an 
            element outside of the motor catalytic domain.
  JOURNAL   Cell. 1997 Sep 5. 90(5):959-66.
  PUBMED    9298907
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1997 
            Sep 5. 90(5):959-66."
COMMENT     Sequence Label: NK-1 GFP
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..28
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(89..107)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      103..150
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(195..213)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        281..385
                     /gene="bla"
                     /label=AmpR promoter
     CDS             570..948
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(604..623)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actatacaaa catcaccatc atcaccatta actcgagcag atccggctgc taacaaagcc
       61 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
      121 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggataattc
      181 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      241 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      301 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      361 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      421 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      481 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      541 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
      601 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
      661 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
      721 ggatggcatg acagtaagag aattatgcag tgctgncata accatgagtg ataacactgc
      781 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
      841 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
      901 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
      961 aactggcgaa cta
//
