LOCUS       sequence-304195-        9508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-LRIG3-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5113..5148
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5158..5181
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6841..6885
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6886..6912
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7052..7069)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7058..7169
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7287..7307)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7292..7621
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7472..7607
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7534..7553
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7669..7716
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7735..8334
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7735..7754)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8231..8251
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8464..8585
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8501..8520)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8555..8574
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8638..9429,9430..9498))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9261..9280
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9499..9508,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccgg aagatctgat
     2761 tctggtggaa gaggcgagct gggccagcct agcggagttg ctgctgaaag accctgtcct
     2821 accacctgta gatgcctggg cgatctgctg gactgcagca gaaagagact ggccagactg
     2881 cctgagcctc tgccttcttg ggttgccaga ctggatctga gccacaaccg gctgagcttc
     2941 atcaaggcca gcagcatgag ccatctgcag agcctgagag aagtgaagct gaacaacaac
     3001 gagctggaaa cgatccccaa tctgggcccc gtgtccgcca atatcacact gctgtctctg
     3061 gccggcaacc ggatcgtgga aatcctgcca gagcacctga aagagttcca gagcctggaa
     3121 accctggacc tgagcagcaa caacatcagc gagctgcaga ccgcctttcc tgctctgcag
     3181 ctgaagtacc tgtacctgaa ctccaacaga gtgaccagca tggaacccgg ctacttcgac
     3241 aacctggcca acactctgct ggtgctgaaa ctgaaccgga acaggatcag cgccattcct
     3301 cctaagatgt tcaagctgcc ccagctgcag cacctggaac tgaacagaaa caagattaag
     3361 aacgtggacg gcctgacctt ccaaggcctg ggagcactga agtccctgaa gatgcagaga
     3421 aacggcgtga ccaagctgat ggacggcgcc ttttggggcc tgagcaacat ggaaattctg
     3481 caactggacc acaacaacct gaccgagatc accaaaggct ggctgtacgg cctgctgatg
     3541 ctgcaagaac tgcacctgag ccagaacgcc atcaacagaa tcagccctga cgcctgggag
     3601 ttctgccaga agctgtctga gctggacctc accttcaacc acctgtccag gctggacgac
     3661 agcagctttc tgggactgag cctgctgaac accctgcaca tcggcaacaa ccgggtgtcc
     3721 tatatcgccg actgcgcctt tagaggcctg tctagcctga aaacactgga tctcaagaac
     3781 aacgagatca gctggaccat cgaggacatg aatggcgcct tcagcggcct ggacaagctg
     3841 cggagactga tcctgcaggg caatagaatc cggtccatca ccaagaaagc ctttaccggc
     3901 ctggatgccc tggaacacct ggatctgtcc gacaatgcca tcatgagcct gcagggaaat
     3961 gccttcagcc agatgaagaa gctccagcag ctgcatctga acaccagcag cctgctgtgc
     4021 gactgccagc tgaaatggct gcctcagtgg gtcgccgaga acaacttcca gagcttcgtg
     4081 aacgccagct gcgcccatcc tcagttgctg aagggcagat ccatcttcgc cgtgtctccc
     4141 gatggcttcg tgtgcgacga tttccccaag cctcagatca ccgtgcagcc cgaaacacag
     4201 tctgccatca agggctccaa cctgtccttc atctgtagcg ccgccagctc cagcgatagc
     4261 cctatgacat tcgcctggaa gaaggataac gagctgctgc acgacgccga gatggaaaac
     4321 tacgctcacc tgagagccca aggcggcgaa gtgatggagt acaccaccat cctgcggctg
     4381 agagaggtgg aatttgccag cgagggcaag taccagtgcg tgatcagcaa ccacttcggc
     4441 agcagctaca gcgtgaaggc caagctgacc gtgaacatgc tgcccagctt caccaagaca
     4501 cccatggatc tgaccatcag agccggcgct atggcccgac ttgaatgtgc tgctgtggga
     4561 caccctgctc ctcagattgc ctggcagaaa gacggcggca ccgattttcc agccgctcgc
     4621 gagagaagaa tgcacgtgat gcccgaggac gacgtgttct tcatcgtgga cgtgaagatc
     4681 gaggatatcg gcgtgtacag ctgcacagcc cagaactctg ccggctccat ctctgccaat
     4741 gccacactga ccgtgctgga aacaccctcc tttctgcggc ccctgctgga tagaacagtg
     4801 accaaaggcg agacagccgt gctgcagtgt attgccggtg gaagccctcc acctaagctg
     4861 aattggacca aggacgactc ccctctggtc gtgaccgaga gacacttttt cgccgctggc
     4921 aaccagctgc tgatcattgt ggatagcgac gtgtccgacg ccggcaagta cacatgcgag
     4981 atgtctaaca ccctgggcac cgagagaggc aacgttcggc tgtctgtgat ccccacacct
     5041 acctgtgaca gccctcagat gacagcccct agcctggatg atgatggctg ggccacagtt
     5101 ggaggcgcgc cggaagatca agttgatcca agattgattg atggcaaggg cggatctctg
     5161 gaggtgctgt tccagggacc aggcggaagc ggcaatcccg gcggcgatct ggccccccag
     5221 atgctgcgcg agctgcagga gaccaatgcc gccctgcagg acgtgcgcga gctgctgcgc
     5281 caccgcgtga aggagatcac cttcctgaag aacaccgtga tggagtgcga cgcctgcggt
     5341 atgcagccct ctagaggctc cggctccatc atcccagttg aggaggagaa cccggacttc
     5401 tggaaccgcg aggcagccga ggccctgggt gccgccaaga agctgcagcc tgcacagaca
     5461 gccgccaaga acctcatcat cttcctgggc gatgggatgg gggtgtctac ggtgacagct
     5521 gccaggatcc taaaagggca gaagaaggac aaactggggc ctgagatacc cctggccatg
     5581 gaccgcttcc catatgtggc tctgtccaag acatacaatg tagacaaaca tgtgccagac
     5641 agtggagcca cagccacggc ctacctgtgc ggggtcaagg gcaacttcca gaccattggc
     5701 ttgagtgcag ccgcccgctt taaccagtgc aacacgacac gcggcaacga ggtcatctcc
     5761 gtgatgaatc gggccaagaa agcagggaag tcagtgggag tggtaaccac cacacgagtg
     5821 cagcacgcct cgccagccgg cacctacgcc cacacggtga accgcaactg gtactcggac
     5881 gccgacgtgc ctgcctcggc ccgccaggag gggtgccagg acatcgctac gcagctcatc
     5941 tccaacatgg acattgacgt gatcctaggt ggaggccgaa agtacatgtt tcgcatggga
     6001 accccagacc ctgagtaccc agatgactac agccaaggtg ggaccaggct ggacgggaag
     6061 aatctggtgc aggaatggct ggcgaagcgc cagggtgccc ggtatgtgtg gaaccgcact
     6121 gagctcatgc aggcttccct ggacccgtct gtgacccatc tcatgggtct ctttgagcct
     6181 ggagacatga aatacgagat ccaccgagac tccacactgg acccctccct gatggagatg
     6241 acagaggctg ccctgcgcct gctgagcagg aacccccgcg gcttcttcct cttcgtggag
     6301 ggtggtcgca tcgaccatgg tcatcatgaa agcagggctt accgggcact gactgagacg
     6361 atcatgttcg acgacgccat tgagagggcg ggccagctca ccagcgagga ggacacgctg
     6421 agcctcgtca ctgccgacca ctcccacgtc ttctccttcg gaggctaccc cctgcgaggg
     6481 agctccatct tcgggctggc ccctggcaag gcccgggaca ggaaggccta cacggtcctc
     6541 ctatacggaa acggtccagg ctatgtgctc aaggacggcg cccggccgga tgttaccgag
     6601 agcgagagcg ggagccccga gtatcggcag cagtcagcag tgcccctgga cgaagagacc
     6661 cacgcaggcg aggacgtggc ggtgttcgcg cgcggcccgc aggcgcacct ggttcacggc
     6721 gtgcaggagc agaccttcat agcgcacgtc atggccttcg ccgcctgcct ggagccctac
     6781 accgcctgcg acctggcgcc ccccgccggc accaccgacg ccgcgcaccc gggtggcgga
     6841 ggactgaacg acatcttcga ggctcagaaa atcgaatggc acgaacatca ccatcaccat
     6901 caccatcacc attaaggccg gcctgtagtc actggcacaa cgcgttaccc ggtaagccaa
     6961 tcgggtatac acggtcgtca tactgcagac agggttcttc tactttgcaa gatagtcttg
     7021 agtagtaaaa taaatagata gagaaaatca gcctcgactg tgccttctag ttgccagcca
     7081 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     7141 ctttcctaat aaaatgagga aattgcatca caacactcaa ccctatctcg gtctattctt
     7201 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7261 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7321 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7381 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7441 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7501 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7561 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     7621 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     7681 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatgacc
     7741 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     7801 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     7861 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     7921 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     7981 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     8041 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     8101 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     8161 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagaca
     8221 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     8281 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacacgtg
     8341 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     8401 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     8461 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8521 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8581 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatta
     8641 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8701 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8761 cgctgcgatg ataccgcgag aaccacgctc accggctccg gatttatcag caataaacca
     8821 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8881 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8941 tgttgccatc gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9001 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9061 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9121 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9181 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9241 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9301 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9361 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9421 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9481 gaaatgttga atactcatat tcttcctt
//
