LOCUS       sequence-304199-        9571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-NOTCH2(Trunc2)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5176..5211
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5221..5244
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6904..6948
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6949..6975
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7115..7132)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7121..7232
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7350..7370)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7355..7684
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7535..7670
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7597..7616
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7732..7779
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7798..8397
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7798..7817)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8294..8314
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8527..8648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8564..8583)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8618..8637
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8701..9492,9493..9561))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9324..9343
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9562..9571,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgcctt gcaatgtaga
     2761 gatggctacg agccctgcgt gaacgaaggg atgtgtgtga cctaccacaa cggcaccggc
     2821 tactgcaagt gccctgaggg atttctgggc gagtactgcc agcacagaga tccctgcgag
     2881 aagaacagat gccagaacgg cggaacctgt gtggcccagg ctatgctggg aaaagccacc
     2941 tgtagatgcg ccagcggctt taccggcgag gattgtcagt acagcaccag ccatccttgc
     3001 ttcgtgtccc ggccttgtct gaatggcggc acctgtcaca tgctgagcag agacacctac
     3061 gagtgtacct gccaagtggg cttcaccggc aaagaatgcc agtggaccga tgcctgcctg
     3121 tctcaccctt gtgccaatgg cagcacatgt accaccgtgg ccaaccagtt cagctgcaag
     3181 tgtctgaccg gcttcacagg ccagaaatgc gagacagacg tgaacgagtg cgacatccct
     3241 ggccactgtc agcatggcgg aacatgcctg aatctgcccg gcagctacca gtgccagtgt
     3301 cctcaaggct tcactggaca gtactgcgac agcctgtacg tgccctgtgc tccttctcct
     3361 tgtgtgaacg gcgggacatg tagacagacc ggcgacttca ccttcgagtg caactgcctg
     3421 ccaggcttcg agggcagcac ttgcgagaga aacatcgacg actgccccaa ccaccggtgc
     3481 caaaatggcg gagtgtgtgt ggatggcgtg aacacctaca actgcagatg ccctcctcag
     3541 tggacaggcc agttctgcac cgaggatgtg gatgagtgtc tgctgcagcc taacgcctgt
     3601 caaaacggcg gcacttgcgc caacagaaat ggcggctatg gctgcgtgtg tgtcaacgga
     3661 tggtccggcg acgattgcag cgagaacatc gatgattgcg ccttcgccag ctgcacccct
     3721 ggctctacct gtatcgatag agtggccagc ttctcctgca tgtgtcccga aggcaaagcc
     3781 ggcctgctgt gccatctgga tgatgcctgc atcagcaacc cctgtcacaa aggcgccctg
     3841 tgcgacacca atcctctgaa cggccagtac atctgcacct gtcctcaggg ctataagggc
     3901 gccgactgta cagaggacgt ggacgaatgt gccatggcca acagcaaccc ttgtgaacac
     3961 gccgggaaat gtgtgaacac cgacggcgcc tttcactgcg agtgcctgaa aggatacgct
     4021 ggccccagat gcgagatgga catcaacgag tgccacagcg acccctgcca gaatgatgcc
     4081 acctgtctgg ataagatcgg cggcttcact tgcctgtgca tgcctggctt taagggcgtg
     4141 cactgcgagc tggaaatcaa tgaatgtcaa agcaacccgt gcgtgaacaa tggccagtgc
     4201 gtggacaaag tgaacaggtt ccagtgtctg tgcccacctg gattcaccgg acctgtgtgc
     4261 cagatcgaca ttgatgactg ctccagcaca ccctgcctga acggcgccaa gtgtatcgat
     4321 caccccaacg gctatgagtg tcagtgtgcc acaggcttta caggcgtgct gtgcgaagag
     4381 aatatcgaca actgcgaccc cgatccttgc caccacggac agtgtcagga tggcatcgac
     4441 agctacacct gtatctgcaa ccccggctac atgggcgcca tctgcagcga tcagatcgat
     4501 gagtgctaca gcagcccatg tctgaacgac ggcagatgca tcgacctggt caatggctat
     4561 cagtgcaatt gccagcctgg caccagcgga gtgaactgcg agatcaactt cgatgactgt
     4621 gccagcaatc cctgtatcca cggcatctgt atggacggca tcaaccggta cagctgtgtg
     4681 tgcagcccag ggtttaccgg ccagagatgc aacattgata tcgacgagtg cgcctccaat
     4741 ccatgcagaa agggcgccac atgcatcaac ggcgtgaacg gcttccggtg catctgtcct
     4801 gaaggacccc accatcctag ctgctacagc caagtgaacg aatgcctgag caacccatgc
     4861 atccacggaa actgtacagg cggcctgagc ggctacaagt gcctgtgtga tgctggctgg
     4921 gtcggaatca actgcgaggt ggacaagaat gagtgcctgt ctaatccctg tcagaatggt
     4981 ggcacatgtg acaatctcgt gaatggctac aggtgcacat gcaagaaggg cttcaagggg
     5041 tacaactgcc aagtcaacat tgacgagtgt gcttctaacc cctgcctcaa ccagggcacc
     5101 tgtttcgacg acatcagcgg ctatacctgc cactgcgtgc tgccttacac cggcaagaac
     5161 tgtcaaggcg cgccggaaga tcaagttgat ccaagattga ttgatggcaa gggcggatct
     5221 ctggaggtgc tgttccaggg accaggcgga agcggcaatc ccggcggcga tctggccccc
     5281 cagatgctgc gcgagctgca ggagaccaat gccgccctgc aggacgtgcg cgagctgctg
     5341 cgccaccgcg tgaaggagat caccttcctg aagaacaccg tgatggagtg cgacgcctgc
     5401 ggtatgcagc cctctagagg ctccggctcc atcatcccag ttgaggagga gaacccggac
     5461 ttctggaacc gcgaggcagc cgaggccctg ggtgccgcca agaagctgca gcctgcacag
     5521 acagccgcca agaacctcat catcttcctg ggcgatggga tgggggtgtc tacggtgaca
     5581 gctgccagga tcctaaaagg gcagaagaag gacaaactgg ggcctgagat acccctggcc
     5641 atggaccgct tcccatatgt ggctctgtcc aagacataca atgtagacaa acatgtgcca
     5701 gacagtggag ccacagccac ggcctacctg tgcggggtca agggcaactt ccagaccatt
     5761 ggcttgagtg cagccgcccg ctttaaccag tgcaacacga cacgcggcaa cgaggtcatc
     5821 tccgtgatga atcgggccaa gaaagcaggg aagtcagtgg gagtggtaac caccacacga
     5881 gtgcagcacg cctcgccagc cggcacctac gcccacacgg tgaaccgcaa ctggtactcg
     5941 gacgccgacg tgcctgcctc ggcccgccag gaggggtgcc aggacatcgc tacgcagctc
     6001 atctccaaca tggacattga cgtgatccta ggtggaggcc gaaagtacat gtttcgcatg
     6061 ggaaccccag accctgagta cccagatgac tacagccaag gtgggaccag gctggacggg
     6121 aagaatctgg tgcaggaatg gctggcgaag cgccagggtg cccggtatgt gtggaaccgc
     6181 actgagctca tgcaggcttc cctggacccg tctgtgaccc atctcatggg tctctttgag
     6241 cctggagaca tgaaatacga gatccaccga gactccacac tggacccctc cctgatggag
     6301 atgacagagg ctgccctgcg cctgctgagc aggaaccccc gcggcttctt cctcttcgtg
     6361 gagggtggtc gcatcgacca tggtcatcat gaaagcaggg cttaccgggc actgactgag
     6421 acgatcatgt tcgacgacgc cattgagagg gcgggccagc tcaccagcga ggaggacacg
     6481 ctgagcctcg tcactgccga ccactcccac gtcttctcct tcggaggcta ccccctgcga
     6541 gggagctcca tcttcgggct ggcccctggc aaggcccggg acaggaaggc ctacacggtc
     6601 ctcctatacg gaaacggtcc aggctatgtg ctcaaggacg gcgcccggcc ggatgttacc
     6661 gagagcgaga gcgggagccc cgagtatcgg cagcagtcag cagtgcccct ggacgaagag
     6721 acccacgcag gcgaggacgt ggcggtgttc gcgcgcggcc cgcaggcgca cctggttcac
     6781 ggcgtgcagg agcagacctt catagcgcac gtcatggcct tcgccgcctg cctggagccc
     6841 tacaccgcct gcgacctggc gccccccgcc ggcaccaccg acgccgcgca cccgggtggc
     6901 ggaggactga acgacatctt cgaggctcag aaaatcgaat ggcacgaaca tcaccatcac
     6961 catcaccatc accattaagg ccggcctgta gtcactggca caacgcgtta cccggtaagc
     7021 caatcgggta tacacggtcg tcatactgca gacagggttc ttctactttg caagatagtc
     7081 ttgagtagta aaataaatag atagagaaaa tcagcctcga ctgtgccttc tagttgccag
     7141 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     7201 gtcctttcct aataaaatga ggaaattgca tcacaacact caaccctatc tcggtctatt
     7261 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7321 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7381 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7441 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7501 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     7561 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     7621 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     7681 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     7741 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     7801 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     7861 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     7921 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     7981 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     8041 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     8101 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     8161 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     8221 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     8281 acatccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     8341 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacac
     8401 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     8461 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8521 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8581 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8641 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     8701 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8761 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8821 cagcgctgcg atgataccgc gagaaccacg ctcaccggct ccggatttat cagcaataaa
     8881 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8941 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9001 cgttgttgcc atcgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9061 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9121 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9181 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9241 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9301 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9361 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9421 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9481 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9541 acggaaatgt tgaatactca tattcttcct t
//
