LOCUS       sequence-304207-        9613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of secreted protein fused to 
            COMP5AP-AviTag-9xHis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-FSTL4-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5218..5253
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5263..5286
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6946..6990
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6991..7017
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7157..7174)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7163..7274
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7392..7412)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7397..7726
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7577..7712
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7639..7658
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7774..7821
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7840..8439
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7840..7859)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8336..8356
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8569..8690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8606..8625)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8660..8679
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8743..9534,9535..9603))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9366..9385
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9604..9613,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgcctg gatggaccct
     2761 ggaacaagta gaggacctga tgtcggcgtg ggagagtctc aggccgagga acctagaagc
     2821 ttcgaagtga ccagaagaga gggcctgagc agccacaatg agctgctggc ctcttgcggc
     2881 aagaagttct gtagcagagg cagcagatgc gtgctgagca gaaagacagg cgagcctgag
     2941 tgtcagtgcc tggaagcctg tagacctagc tacgtgccag tgtgtggcag cgacggcaga
     3001 ttctacgaga accactgcaa actgcacaga gccgcctgtc tgctgggcaa gagaatcacc
     3061 gtgatccaca gcaaggactg ctttctgaag ggcgatacct gcacaatggc cggctacgcc
     3121 agactgaaaa acgtgctgct ggcactgcag accagactgc aacctctgca agagggcgat
     3181 agtagacagg accccgccag ccagaaaaga ctgctggtgg aaagcctgtt cagagatctg
     3241 gacgccgatg gcaatggcca cctgagttca tctgaactgg cccagcatgt gctgaagaag
     3301 caggacctgg acgaggacct gcttggctgt tctcctggcg acctgctgag attcgacgac
     3361 tacaacagcg acagcagcct gacactgcgc gagttctaca tggcctttca ggtggtgcag
     3421 ctgtctctgg cccctgagga tagagtgtct gtgaccacag tgaccgtggg cctgtctaca
     3481 gtgctgacat gtgctgtgca tggcgatctg cggcctccaa tcatctggaa gagaaacgga
     3541 ctgaccctga acttcctgga cctggaagat atcaacgact tcggcgagga cgacagcctg
     3601 tacatcacca aagtgaccac catccacatg ggcaactaca cctgtcacgc ctctggccac
     3661 gagcagctgt ttcagactca cgtgctgcaa gtgaacgtgc cacctgtgat cagagtgtac
     3721 cccgagagcc aagctcaaga acctggcgtg gcagcctctc tgagatgtca tgccgagggc
     3781 atccccatgc ctagaatcac ctggctgaag aacggcgtgg acgtgtccac acagatgagc
     3841 aaacaactgt ccctgctggc caatggcagc gagctgcaca tcagcagcgt cagatatgag
     3901 gacaccggcg cctacacatg tatcgccaag aatgaagtgg gcgtcgacga ggacatcagc
     3961 tccctgttta tcgaggacag cgccagaaag accctggcca acatcctttg gagagaagag
     4021 ggactgagcg tgggcaacat gttctacgtg ttcagcgacg acggcatcat cgtgattcac
     4081 cccgtggact gcgagatcca gagacacctg aagcctaccg agaagatctt tatgagctac
     4141 gaggaaatct gcccgcagag agagaagaac gccacacagc cttgtcagtg ggtgtcagcc
     4201 gtgaatgtgc ggaaccggta catctatgtg gcccagccag ctctgagtag agtgctggtg
     4261 gtggacattc aggcccagaa ggtgctgcag tctatcggag ttgatcccct gcctgccaag
     4321 ctgtcctacg acaagtctca cgaccaagtg tgggtgctga gctggggcga cgttcacaaa
     4381 agcagaccca gcctgcaagt catcaccgag gcctctacag gacagagcca gcacctgatc
     4441 agaacccctt ttgccggcgt tgacgacttc ttcatccctc ctaccaacct gatcatcaac
     4501 cacatccgct tcggcttcat cttcaacaag agcgaccctg ccgtgcacaa agtggatctg
     4561 gaaaccatga tgcccctgaa aaccatcggc ctgcaccatc acggatgcgt gccacaggct
     4621 atggcccata cacacctcgg cggctacttt ttcatccagt gcagacagga tagccccgcc
     4681 tctgctgcta gacagcttct ggtggatagc gtgaccgata gcgtgctggg acctaatggg
     4741 gatgtgaccg gcacacctca cacaagccct gatggcagat tcatcgtgtc tgccgccgct
     4801 gattctccct ggctgcatgt gcaagagatt acagtgcggg gcgagattca gaccctgtac
     4861 gacctgcaga tcaacagcgg catcagcgac ctggccttcc agagaagctt caccgagagc
     4921 aaccagtaca acatctacgc cgctctgcac accgagcctg acctgctgtt tctggaactg
     4981 agcaccggca aagtgggcat gctgaaaaac ctgaaagagc ctcctgccgg acctgctcaa
     5041 ccttggggag gaacccacag aatcatgaga gatagcggcc tgttcggcca gtacctgctg
     5101 acaccagcca gagagagcct gttcctgatc aacggcagac agaacaccct gagatgcgag
     5161 gtgtccggca tcaaaggcgg cacaacagtc gtgtgggtcg gagaagttgg cgcgccggaa
     5221 gatcaagttg atccaagatt gattgatggc aagggcggat ctctggaggt gctgttccag
     5281 ggaccaggcg gaagcggcaa tcccggcggc gatctggccc cccagatgct gcgcgagctg
     5341 caggagacca atgccgccct gcaggacgtg cgcgagctgc tgcgccaccg cgtgaaggag
     5401 atcaccttcc tgaagaacac cgtgatggag tgcgacgcct gcggtatgca gccctctaga
     5461 ggctccggct ccatcatccc agttgaggag gagaacccgg acttctggaa ccgcgaggca
     5521 gccgaggccc tgggtgccgc caagaagctg cagcctgcac agacagccgc caagaacctc
     5581 atcatcttcc tgggcgatgg gatgggggtg tctacggtga cagctgccag gatcctaaaa
     5641 gggcagaaga aggacaaact ggggcctgag atacccctgg ccatggaccg cttcccatat
     5701 gtggctctgt ccaagacata caatgtagac aaacatgtgc cagacagtgg agccacagcc
     5761 acggcctacc tgtgcggggt caagggcaac ttccagacca ttggcttgag tgcagccgcc
     5821 cgctttaacc agtgcaacac gacacgcggc aacgaggtca tctccgtgat gaatcgggcc
     5881 aagaaagcag ggaagtcagt gggagtggta accaccacac gagtgcagca cgcctcgcca
     5941 gccggcacct acgcccacac ggtgaaccgc aactggtact cggacgccga cgtgcctgcc
     6001 tcggcccgcc aggaggggtg ccaggacatc gctacgcagc tcatctccaa catggacatt
     6061 gacgtgatcc taggtggagg ccgaaagtac atgtttcgca tgggaacccc agaccctgag
     6121 tacccagatg actacagcca aggtgggacc aggctggacg ggaagaatct ggtgcaggaa
     6181 tggctggcga agcgccaggg tgcccggtat gtgtggaacc gcactgagct catgcaggct
     6241 tccctggacc cgtctgtgac ccatctcatg ggtctctttg agcctggaga catgaaatac
     6301 gagatccacc gagactccac actggacccc tccctgatgg agatgacaga ggctgccctg
     6361 cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg tcgcatcgac
     6421 catggtcatc atgaaagcag ggcttaccgg gcactgactg agacgatcat gttcgacgac
     6481 gccattgaga gggcgggcca gctcaccagc gaggaggaca cgctgagcct cgtcactgcc
     6541 gaccactccc acgtcttctc cttcggaggc taccccctgc gagggagctc catcttcggg
     6601 ctggcccctg gcaaggcccg ggacaggaag gcctacacgg tcctcctata cggaaacggt
     6661 ccaggctatg tgctcaagga cggcgcccgg ccggatgtta ccgagagcga gagcgggagc
     6721 cccgagtatc ggcagcagtc agcagtgccc ctggacgaag agacccacgc aggcgaggac
     6781 gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca ggagcagacc
     6841 ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc cctacaccgc ctgcgacctg
     6901 gcgccccccg ccggcaccac cgacgccgcg cacccgggtg gcggaggact gaacgacatc
     6961 ttcgaggctc agaaaatcga atggcacgaa catcaccatc accatcacca tcaccattaa
     7021 ggccggcctg tagtcactgg cacaacgcgt tacccggtaa gccaatcggg tatacacggt
     7081 cgtcatactg cagacagggt tcttctactt tgcaagatag tcttgagtag taaaataaat
     7141 agatagagaa aatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     7201 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     7261 gaggaaattg catcacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7321 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7381 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     7441 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     7501 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     7561 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7621 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     7681 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     7741 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     7801 atcggcatag tataatacga caaggtgagg aactaaacca tgaccgagta caagcccacg
     7861 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     7921 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     7981 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     8041 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     8101 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     8161 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     8221 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     8281 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacatccgc gccccgcaac
     8341 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     8401 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac acgtgctacg agatttcgat
     8461 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     8521 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     8581 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8641 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     8701 ataccgtcga cctctagcta gagcttggcg taatcatggt cattaccaat gcttaatcag
     8761 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8821 cgtgtagata actacgatac gggagggctt accatctggc cccagcgctg cgatgatacc
     8881 gcgagaacca cgctcaccgg ctccggattt atcagcaata aaccagccag ccggaagggc
     8941 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9001 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccatcgctac
     9061 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9121 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9181 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9241 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9301 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9361 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9421 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9481 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9541 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9601 catattcttc ctt
//
