LOCUS       sequence-304215-        9661 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of secreted protein fused to 
            COMP5AP-AviTag-9xHis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-IGSF22-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5266..5301
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5311..5334
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6994..7038
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7039..7065
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7205..7222)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7211..7322
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7440..7460)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7445..7774
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7625..7760
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7687..7706
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7822..7869
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7888..8487
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7888..7907)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8384..8404
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8617..8738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8654..8673)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8708..8727
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8791..9582,9583..9651))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9414..9433
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9652..9661,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccga gtttgtggaa
     2761 aaacctcagc ctgtgacagc ccctgaaggc gacaaagccg tgtttagagc cagagtgcag
     2821 ggcaacgcca agcctcacat cagctggaag agagagagcg gcatccccat caaagagagc
     2881 gccaagatct tctacgacag catcaacaaa gagcacgtcc tgaagctgga acccctgacc
     2941 agcgacgaca gcgacaacta caagtgtatc gccagcaacg accacgccga cgccatctat
     3001 acagtgtccc tgctggttac cgagggccaa gagaagatgg acttcaagaa gatgctgaag
     3061 aagagagccc ctccggctcc taagaaaaag cagaaaaagg tggccaacga gaaagagatg
     3121 ctcgagatcc tgagcaaggt gcccaagaaa gacttcgaga aagtgtgcat ggaatacggc
     3181 ttcaccgact tccggggcct gctgcggaag ctgaaagaaa tgaagaagaa ggtcgaggtc
     3241 gaggccatca gaatcctgaa gcctctcgag gacaaagaga caaaggtcga cacaaccgtg
     3301 gtgttcgact gcatcatgga actgaaggac cccaacgtga agatgatctg gatcaagggc
     3361 accgagcctc tgagaatcca gtacagcctg gggaagtacg acgtgaagca gatgggcacc
     3421 aagtacatgc tggtcatcag caacgtcaac atgaacgacg ccggcatcta cagcctgagc
     3481 gtgggcgata agagaatgag cgccgagctg acagtgctgg acgagccact gaagtttctg
     3541 ggcgagatga agcccgtgaa agtgaccgaa agacagaccg ccgtgttcga gatccggctg
     3601 agcaagaaag aacccaactt cgtgtggaag ttcaacggca aagagctgaa gcgggacgat
     3661 aagtacgaga tcaccgtgtc cgaggacggc ctgacacaca ccctgaagat caaggacgcc
     3721 agactgagcg atagcggcga gttttctgcc gaggctggca atctggtgca gaaagcccag
     3781 ctgaccgtgg acagaatccc tatcaagttc gtgtccaacc tgaagaacgt gcgcgtgaaa
     3841 gagagaagca gagcctgcct ggaatgcgag ctgaccagca aggatgtgac cctgagatgg
     3901 aagaaggacg gccagctgct gatgcacggc acaaagtaca gcatgaacca cgaaggcaag
     3961 cgggccgaac tgatcattga ggatgctcag ctgtctgacg gcggcgagta tacagtggtt
     4021 gccatgcagg atggcgaccc caccgagtac tatagcacag ccatcgtgac agtggaagaa
     4081 cggctggcca cagtgaagtc tggcatgtct gatgtgcacg ccgccacagg atctccagct
     4141 gaactgtgcg tggtgctgaa cgacgaaaag gtggaaggcg tgtggctgaa ggatggcaaa
     4201 gagatcacag atctgcccgg catgcagatt gtgaaacagg gcgccgtgca caagctgatc
     4261 ttcccttcta tgggccccga acacgagggc aagtacacat tcagagccaa gggcacagag
     4321 tccgaggcct ccgtgtttat cgccgatcct cctaccatcg atcccagcgt tctggaagct
     4381 ctggccgctc acgccatcac agtgaaagtg ggacacacag cccacatcaa ggtgccattc
     4441 agagggaagc ccctgcctaa agtgacctgg tacaaggacg gcatggaagt gacagaagag
     4501 gaacgcgtca gcatggaaag aggcgaagat caggccctgc tgaccatctc caattgcgtg
     4561 cgggaagata gcggcctgat cctgctgaaa ctgaagaatg atcacggcag cgccaccgcc
     4621 acactgcatc tgtctgttct ggaaccacct ggcttcgcca gccagcctca agtgactgat
     4681 gtgaccaaag aagccgtgac catcacctgg aacgccccta cacaagatgg cggagcacct
     4741 gtgctgggct acatcgtgga aagacggaag aaaggcagca acctgtgggt gcccgtgaac
     4801 aaggacccta tccagggcac caaatgcacc gtggatggcc tgctggaaga taccgagtac
     4861 gagttcagag tgatcgccgt caacaaggcc ggacctggac agccttctgt gcctagttct
     4921 agcgtggtgg ctaaggaccc tgtgaagcct cctggactgg tgcaggatct gcacgtgtcc
     4981 gatagcagca acagcagcat cagcctggct tggagagaac ctgcagaggg cgatcctcca
     5041 agcggctata tcctggaaat gagagccgag gacaccaaag aatggtccaa gtgcaccaag
     5101 attcccatca gcggcacctg ttataccgtc ggcggactga tcgagcggca gaagtacttt
     5161 ttccggatca gagccgtgaa tgaggccggc gtgggagaac ctgttgaact ggataagggc
     5221 gtcagagcca tgcctccacc tggcctgaca acaacaggcg cgccggaaga tcaagttgat
     5281 ccaagattga ttgatggcaa gggcggatct ctggaggtgc tgttccaggg accaggcgga
     5341 agcggcaatc ccggcggcga tctggccccc cagatgctgc gcgagctgca ggagaccaat
     5401 gccgccctgc aggacgtgcg cgagctgctg cgccaccgcg tgaaggagat caccttcctg
     5461 aagaacaccg tgatggagtg cgacgcctgc ggtatgcagc cctctagagg ctccggctcc
     5521 atcatcccag ttgaggagga gaacccggac ttctggaacc gcgaggcagc cgaggccctg
     5581 ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat catcttcctg
     5641 ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg gcagaagaag
     5701 gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt ggctctgtcc
     5761 aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac ggcctacctg
     5821 tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg ctttaaccag
     5881 tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa gaaagcaggg
     5941 aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc cggcacctac
     6001 gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc ggcccgccag
     6061 gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga cgtgatccta
     6121 ggtggaggcc gaaagtacat gtttcgcatg ggaaccccag accctgagta cccagatgac
     6181 tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg gctggcgaag
     6241 cgccagggtg cccggtatgt gtggaaccgc actgagctca tgcaggcttc cctggacccg
     6301 tctgtgaccc atctcatggg tctctttgag cctggagaca tgaaatacga gatccaccga
     6361 gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg cctgctgagc
     6421 aggaaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca tggtcatcat
     6481 gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc cattgagagg
     6541 gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga ccactcccac
     6601 gtcttctcct tcggaggcta ccccctgcga gggagctcca tcttcgggct ggcccctggc
     6661 aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc aggctatgtg
     6721 ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc cgagtatcgg
     6781 cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt ggcggtgttc
     6841 gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt catagcgcac
     6901 gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc gccccccgcc
     6961 ggcaccaccg acgccgcgca cccgggtggc ggaggactga acgacatctt cgaggctcag
     7021 aaaatcgaat ggcacgaaca tcaccatcac catcaccatc accattaagg ccggcctgta
     7081 gtcactggca caacgcgtta cccggtaagc caatcgggta tacacggtcg tcatactgca
     7141 gacagggttc ttctactttg caagatagtc ttgagtagta aaataaatag atagagaaaa
     7201 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     7261 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     7321 tcacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     7381 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     7441 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7501 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     7561 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     7621 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7681 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     7741 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     7801 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     7861 taatacgaca aggtgaggaa ctaaaccatg accgagtaca agcccacggt gcgcctcgcc
     7921 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     7981 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     8041 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     8101 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     8161 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     8221 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     8281 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     8341 gagcgcgccg gggtgcccgc cttcctggag acatccgcgc cccgcaacct ccccttctac
     8401 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     8461 tgcatgaccc gcaagcccgg tgcctgacac gtgctacgag atttcgattc caccgccgcc
     8521 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     8581 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     8641 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8701 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     8761 tctagctaga gcttggcgta atcatggtca ttaccaatgc ttaatcagtg aggcacctat
     8821 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8881 tacgatacgg gagggcttac catctggccc cagcgctgcg atgataccgc gagaaccacg
     8941 ctcaccggct ccggatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9001 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9061 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc atcgctacag gcatcgtggt
     9121 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9181 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9241 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9301 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9361 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9421 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9481 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9541 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9601 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tattcttcct
     9661 t
//
