LOCUS       sequence-304227-        9754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-DSCAML1(Trunc2)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5359..5394
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5404..5427
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7087..7131
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7132..7158
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7298..7315)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7304..7415
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7533..7553)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7538..7867
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7718..7853
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7780..7799
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7915..7962
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7981..8580
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7981..8000)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8477..8497
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8710..8831
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8747..8766)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8801..8820
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8884..9675,9676..9744))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9507..9526
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9745..9754,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccga agatgtgggc
     2761 acaagcctgt acttcgtgaa cgacagcctg cagcaagtga cctttagcag ctctgtgggc
     2821 gtcgtggttc cttgtcctgc tgctggatct ccatctgccg ctctgagatg gtatctggcc
     2881 accggcgacg acatctacga tgtgcctcac attagacacg tgcacgccaa tggcaccctg
     2941 cagctgtacc cttttagccc cagcgccttc aacagcttca tccacgacaa cgactatttc
     3001 tgcaccgccg agaacgccgc tggcaagatc agatccccta acatcagagt gaaggccgtg
     3061 ttccgcgagc cttacaccgt cagagtggaa gatcagcgga gcatgagagg caacgtggcc
     3121 gtgtttaagt gtctgatccc cagcagcgtg caagagtacg tgtccgtggt gtcctgggag
     3181 aaagacaccg tgtctatcat ccccgagcac cggttcttca tcacatacca cggcggcctg
     3241 tacatcagcg acgtgcagaa agaagatgcc ctgagcacct accggtgcat caccaagcac
     3301 aagtacagcg gcgagacaag acagagcaat ggcgccagac tgagcgtgac agatcctgcc
     3361 gagagcatcc ctaccatcct ggatggcttc cacagccaag aagtgtgggc cggacatacc
     3421 gtggaactgc cttgtacagc cagcggctac cccattcctg ccatcagatg gctgaaggac
     3481 ggtagacctc tgcctgccga tagccggtgg acaaagagaa tcaccggcct gaccatctcc
     3541 gacctgagaa cagaggatag cggcacctac atctgcgaag tgaccaatac ctttggcagc
     3601 gccgaggcca caggcatcct gatggttatt gaccctctgc acgtgaccct gacacctaag
     3661 aagctgaaaa ccggcatcgg ctccaccgtg atcctgtctt gtgctctgac cggctctccc
     3721 gagttcacca tcaggtggta cagaaacacc gagctggtgc tgcctgacga ggccatctct
     3781 atcagaggcc tgagcaacga gacactgctg atcacaagcg cccagaagtc tcacagcggc
     3841 gcctaccagt gctttgccac aagaaaagcc cagacagccc aggacttcgc catcattgcc
     3901 ctggaagatg gcacccctag aatcgtgtcc agcttcagcg agaaggtggt caaccctggc
     3961 gagcagtttt ctctgatgtg tgccgctaaa ggcgcccctc cacctacagt gacatgggcc
     4021 ctcgatgatg agcccatcgt cagagatggc agccacagga ccaaccagta caccatgtcc
     4081 gatggcacca ccatcagcca catgaacgtg acaggcccac agataagaga tggcggcgtg
     4141 tacagatgca cagccagaaa tctcgtgggc tccgccgagt accaggccag aatcaatgtt
     4201 cggggccctc ctagcatccg ggccatgaga aatattacag ccgtggccgg cagagacacc
     4261 ctgatcaatt gcagagtgat cggctaccct tactacagca tcaagtggta taaggacgct
     4321 ctgctgctgc ccgacaacca cagacaggtg gtgttcgaga acggcacact gaagctgacc
     4381 gatgtccaga aaggcatgga cgagggcgag tacctgtgct ctgttctgat ccagccacag
     4441 ctgagcatca gccagtctgt gcatgtggcc gtgaaggtgc cacctctgat tcagcctttc
     4501 gagttccctc ctgcctctat cggccagctg ctgtacatcc cttgtgtggt gtctagcggc
     4561 gacatgccca tcagaatcac atggcggaag gatggccaag tgatcatcag cggctctggc
     4621 gtgaccatcg agagcaaaga attcatgtct agcctgcaga tctccagcgt gtccctgaag
     4681 cacaacggca actacacctg tatcgccagc aatgccgccg ctaccgttag cagagaaagg
     4741 cagctgatcg tgcgggtgcc accaagattt gtggtgcagc ccaacaacca ggacggcatc
     4801 tatggaaagg ccggcgtgct gaactgtagc gtggacggct atccacctcc taaagtgatg
     4861 tggaagcacg ccaaaggctc cggcaatcct cagcagtatc accctgtgcc actgaccggc
     4921 agaattcaga ttctgcccaa cagcagcctg ctgatccggc acgtgctgga agaggatatc
     4981 ggctactatc tgtgccaggc ctctaacggc gtgggcaccg atatcagcaa gagcatgttc
     5041 ctgaccgtga agatccccgc catgatcacc agccatccta acaccacaat cgccatcaag
     5101 ggccatgcca aagagctgaa ttgcaccgcc agaggcgagc ggcccatcat cattagatgg
     5161 gagaagggcg ataccgtgat cgaccccgac agagtgatga gatacgctat cgccaccaag
     5221 gacaacggcg acgaagtggt gtcaacactg aaactgaagc ccgccgacag aggcgacagc
     5281 gtgttcttta gctgccacgc catcaactcc tacggcgagg atagaggact catccagctg
     5341 accgtgcaag gcgcgccgga agatcaagtt gatccaagat tgattgatgg caagggcgga
     5401 tctctggagg tgctgttcca gggaccaggc ggaagcggca atcccggcgg cgatctggcc
     5461 ccccagatgc tgcgcgagct gcaggagacc aatgccgccc tgcaggacgt gcgcgagctg
     5521 ctgcgccacc gcgtgaagga gatcaccttc ctgaagaaca ccgtgatgga gtgcgacgcc
     5581 tgcggtatgc agccctctag aggctccggc tccatcatcc cagttgagga ggagaacccg
     5641 gacttctgga accgcgaggc agccgaggcc ctgggtgccg ccaagaagct gcagcctgca
     5701 cagacagccg ccaagaacct catcatcttc ctgggcgatg ggatgggggt gtctacggtg
     5761 acagctgcca ggatcctaaa agggcagaag aaggacaaac tggggcctga gatacccctg
     5821 gccatggacc gcttcccata tgtggctctg tccaagacat acaatgtaga caaacatgtg
     5881 ccagacagtg gagccacagc cacggcctac ctgtgcgggg tcaagggcaa cttccagacc
     5941 attggcttga gtgcagccgc ccgctttaac cagtgcaaca cgacacgcgg caacgaggtc
     6001 atctccgtga tgaatcgggc caagaaagca gggaagtcag tgggagtggt aaccaccaca
     6061 cgagtgcagc acgcctcgcc agccggcacc tacgcccaca cggtgaaccg caactggtac
     6121 tcggacgccg acgtgcctgc ctcggcccgc caggaggggt gccaggacat cgctacgcag
     6181 ctcatctcca acatggacat tgacgtgatc ctaggtggag gccgaaagta catgtttcgc
     6241 atgggaaccc cagaccctga gtacccagat gactacagcc aaggtgggac caggctggac
     6301 gggaagaatc tggtgcagga atggctggcg aagcgccagg gtgcccggta tgtgtggaac
     6361 cgcactgagc tcatgcaggc ttccctggac ccgtctgtga cccatctcat gggtctcttt
     6421 gagcctggag acatgaaata cgagatccac cgagactcca cactggaccc ctccctgatg
     6481 gagatgacag aggctgccct gcgcctgctg agcaggaacc cccgcggctt cttcctcttc
     6541 gtggagggtg gtcgcatcga ccatggtcat catgaaagca gggcttaccg ggcactgact
     6601 gagacgatca tgttcgacga cgccattgag agggcgggcc agctcaccag cgaggaggac
     6661 acgctgagcc tcgtcactgc cgaccactcc cacgtcttct ccttcggagg ctaccccctg
     6721 cgagggagct ccatcttcgg gctggcccct ggcaaggccc gggacaggaa ggcctacacg
     6781 gtcctcctat acggaaacgg tccaggctat gtgctcaagg acggcgcccg gccggatgtt
     6841 accgagagcg agagcgggag ccccgagtat cggcagcagt cagcagtgcc cctggacgaa
     6901 gagacccacg caggcgagga cgtggcggtg ttcgcgcgcg gcccgcaggc gcacctggtt
     6961 cacggcgtgc aggagcagac cttcatagcg cacgtcatgg ccttcgccgc ctgcctggag
     7021 ccctacaccg cctgcgacct ggcgcccccc gccggcacca ccgacgccgc gcacccgggt
     7081 ggcggaggac tgaacgacat cttcgaggct cagaaaatcg aatggcacga acatcaccat
     7141 caccatcacc atcaccatta aggccggcct gtagtcactg gcacaacgcg ttacccggta
     7201 agccaatcgg gtatacacgg tcgtcatact gcagacaggg ttcttctact ttgcaagata
     7261 gtcttgagta gtaaaataaa tagatagaga aaatcagcct cgactgtgcc ttctagttgc
     7321 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     7381 actgtccttt cctaataaaa tgaggaaatt gcatcacaac actcaaccct atctcggtct
     7441 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7501 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     7561 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7621 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7681 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7741 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7801 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7861 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     7921 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     7981 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     8041 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     8101 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     8161 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     8221 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     8281 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     8341 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     8401 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     8461 gagacatccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     8521 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     8581 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     8641 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     8701 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8761 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8821 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     8881 tcattaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8941 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9001 ccccagcgct gcgatgatac cgcgagaacc acgctcaccg gctccggatt tatcagcaat
     9061 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9121 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9181 caacgttgtt gccatcgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9241 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9301 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9361 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9421 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9481 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9541 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9601 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     9661 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     9721 gacacggaaa tgttgaatac tcatattctt cctt
//
