LOCUS       sequence-304229-        9760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9760)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PXDN(Trunc3)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5365..5400
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5410..5433
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7093..7137
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7138..7164
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7304..7321)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7310..7421
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7539..7559)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7544..7873
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7724..7859
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7786..7805
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7921..7968
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7987..8586
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7987..8006)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8483..8503
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8716..8837
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8753..8772)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8807..8826
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8890..9681,9682..9750))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9513..9532
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9751..9760,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccgt taatgtgcct
     2761 gacgtgtcca gaaacggcga cccttttgtg gccaccagca tcgtggaagc cattgccaca
     2821 gtggaccggg ccatcaacag caccagaaca cacctgttcg acagccggcc tagaagcccc
     2881 aatgatctgc tggccctgtt cagataccct cgggaccctt acacagtgga acaggccaga
     2941 gccggcgaga tcttcgagag aacactgcag ctgatccaag agcacgtgca gcacggcctg
     3001 atggtggatc tgaatggcac cagctaccac tacaacgacc tggtgtcccc acagtacctg
     3061 aacctgatcg ccaatctgag cggctgcaca gcccacagaa gagtgaacaa ctgcagcgac
     3121 atgtgcttcc accagaagta cagaacccac gacggcacct gtaacaacct gcagcaccct
     3181 atgtggggcg ccagcctgac agcctttgag agactgctga agtccgtgta cgagaacggc
     3241 ttcaacaccc ctcggggcat caaccctcac cggctgtata atggacacgc cctgcctatg
     3301 cctcggctgg tgtctacaac actgatcggc accgagacag tgacccctga cgagcagttt
     3361 acccacatgc tgatgcagtg gggccagttc ctggaccacg atctggattc tacagtggtg
     3421 gccctgagcc aggccagatt ttctgatggc cagcactgca gcaacgtgtg cagcaatgac
     3481 cctccttgct tcagcgtgat gatccctcct aacgacagca gagccagatc cggcgccaga
     3541 tgcatgttct ttgtgcggag cagccctgtg tgtggcagcg gcatgacaag cctgctgatg
     3601 aacagcgtgt accccagaga gcagatcaac cagctgacct cctacatcga cgcctccaac
     3661 gtgtacggca gcacagagca cgaggcccgc tctattagag atctggcctc tcacagaggc
     3721 ctgctgagac agggaatcgt gcagagaagc ggcaagcccc tgctgccttt tgctaccgga
     3781 cctccaaccg agtgcatgcg ggatgagaac gagagcccca ttccttgctt tctggccggc
     3841 gaccacagag ccaatgaaca gctgggcctg accagcatgc acaccctgtg gttcagagag
     3901 cacaaccgga tcgccaccga gctgctgaag ctgaaccctc actgggacgg cgacaccatc
     3961 tactacgaga cacggaagat cgtgggcgcc gagatccagc acatcaccta tcagcactgg
     4021 ctgcccaaga tcctgggcga agtgggaatg agaaccctgg gcgagtacca cggctacgac
     4081 cctggaatca acgccggcat cttcaacgcc tttgccaccg ccgccttcag attcggccac
     4141 acactggtca accctctgct gtacagactg gacgagaact tccagcctat cgctcaggac
     4201 catctgcctc tgcacaaggc tttcttcagc cccttccgga tcgtgaacga aggcggcatt
     4261 gacccactgc tgagaggcct gtttggagtg gccggcaaga tgagagtgcc tagccagctg
     4321 ctcaataccg agctgaccga gcggctgttt agcatggccc atacagtggc tctggacctg
     4381 gccgccatca atatccagag aggcagagat cacggcatcc ctccatacca cgactaccgg
     4441 gtgtactgca atctgtccgc cgctcacacc ttcgaggacc tgaagaacga gatcaagaac
     4501 cccgagatcc gcgagaagct gaagcggctg tatggctcta ccctgaacat cgatctgttc
     4561 cccgctctgg tggtggaaga tctggtgcct ggaagcagac tgggccccac actgatgtgt
     4621 ctgctgagca cccagttcaa gcggctgaga gatggcgacc ggctttggta cgaaaacccc
     4681 ggtgtcttta gccccgctca gctgacccag atcaagcaga ccagcctggc cagaatcctg
     4741 tgcgacaacg ccgacaacat caccagagtg cagagcgacg tgttcagagt ggccgagttt
     4801 cctcacggct atggcagctg tgacgagatc cccagagtgg acctgagagt gtggcaggac
     4861 tgctgcgagg actgcagaac cagaggccag ttcaatgcct tcagctatca cttccggggc
     4921 agaagaagcc tcgagttcag ctaccaagag gacaagccca ccaagaaaac ccggcctcgg
     4981 aagatcccaa gcgttggcag acaaggcgag cacctgagca atagcaccag cgccttctcc
     5041 accagatccg atgccagcgg caccaacgac ttccgcgagt tcgtgctgga aatgcagaaa
     5101 accatcaccg acctgcggac ccagattaag aagctggaaa gccgcctgag caccaccgag
     5161 tgtgttgatg ctggcggaga gagccacgcc aacaacacca agtggaagaa ggacgcctgc
     5221 accatctgcg agtgcaagga tggccaagtg acctgctttg tggaagcctg tcctcctgcc
     5281 acttgtgccg tgcctgtgaa tatccctggc gcctgctgtc ctgtgtgcct gcagaaaaga
     5341 gccgaggaaa agcctggcgc gccggaagat caagttgatc caagattgat tgatggcaag
     5401 ggcggatctc tggaggtgct gttccaggga ccaggcggaa gcggcaatcc cggcggcgat
     5461 ctggcccccc agatgctgcg cgagctgcag gagaccaatg ccgccctgca ggacgtgcgc
     5521 gagctgctgc gccaccgcgt gaaggagatc accttcctga agaacaccgt gatggagtgc
     5581 gacgcctgcg gtatgcagcc ctctagaggc tccggctcca tcatcccagt tgaggaggag
     5641 aacccggact tctggaaccg cgaggcagcc gaggccctgg gtgccgccaa gaagctgcag
     5701 cctgcacaga cagccgccaa gaacctcatc atcttcctgg gcgatgggat gggggtgtct
     5761 acggtgacag ctgccaggat cctaaaaggg cagaagaagg acaaactggg gcctgagata
     5821 cccctggcca tggaccgctt cccatatgtg gctctgtcca agacatacaa tgtagacaaa
     5881 catgtgccag acagtggagc cacagccacg gcctacctgt gcggggtcaa gggcaacttc
     5941 cagaccattg gcttgagtgc agccgcccgc tttaaccagt gcaacacgac acgcggcaac
     6001 gaggtcatct ccgtgatgaa tcgggccaag aaagcaggga agtcagtggg agtggtaacc
     6061 accacacgag tgcagcacgc ctcgccagcc ggcacctacg cccacacggt gaaccgcaac
     6121 tggtactcgg acgccgacgt gcctgcctcg gcccgccagg aggggtgcca ggacatcgct
     6181 acgcagctca tctccaacat ggacattgac gtgatcctag gtggaggccg aaagtacatg
     6241 tttcgcatgg gaaccccaga ccctgagtac ccagatgact acagccaagg tgggaccagg
     6301 ctggacggga agaatctggt gcaggaatgg ctggcgaagc gccagggtgc ccggtatgtg
     6361 tggaaccgca ctgagctcat gcaggcttcc ctggacccgt ctgtgaccca tctcatgggt
     6421 ctctttgagc ctggagacat gaaatacgag atccaccgag actccacact ggacccctcc
     6481 ctgatggaga tgacagaggc tgccctgcgc ctgctgagca ggaacccccg cggcttcttc
     6541 ctcttcgtgg agggtggtcg catcgaccat ggtcatcatg aaagcagggc ttaccgggca
     6601 ctgactgaga cgatcatgtt cgacgacgcc attgagaggg cgggccagct caccagcgag
     6661 gaggacacgc tgagcctcgt cactgccgac cactcccacg tcttctcctt cggaggctac
     6721 cccctgcgag ggagctccat cttcgggctg gcccctggca aggcccggga caggaaggcc
     6781 tacacggtcc tcctatacgg aaacggtcca ggctatgtgc tcaaggacgg cgcccggccg
     6841 gatgttaccg agagcgagag cgggagcccc gagtatcggc agcagtcagc agtgcccctg
     6901 gacgaagaga cccacgcagg cgaggacgtg gcggtgttcg cgcgcggccc gcaggcgcac
     6961 ctggttcacg gcgtgcagga gcagaccttc atagcgcacg tcatggcctt cgccgcctgc
     7021 ctggagccct acaccgcctg cgacctggcg ccccccgccg gcaccaccga cgccgcgcac
     7081 ccgggtggcg gaggactgaa cgacatcttc gaggctcaga aaatcgaatg gcacgaacat
     7141 caccatcacc atcaccatca ccattaaggc cggcctgtag tcactggcac aacgcgttac
     7201 ccggtaagcc aatcgggtat acacggtcgt catactgcag acagggttct tctactttgc
     7261 aagatagtct tgagtagtaa aataaataga tagagaaaat cagcctcgac tgtgccttct
     7321 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7381 actcccactg tcctttccta ataaaatgag gaaattgcat cacaacactc aaccctatct
     7441 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7501 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     7561 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7621 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7681 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7741 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7801 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7861 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     7921 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     7981 taaaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     8041 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     8101 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     8161 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     8221 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     8281 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     8341 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     8401 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     8461 ttcctggaga catccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     8521 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     8581 gcctgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     8641 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     8701 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8761 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8821 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     8881 tcatggtcat taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8941 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9001 atctggcccc agcgctgcga tgataccgcg agaaccacgc tcaccggctc cggatttatc
     9061 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9121 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9181 tttgcgcaac gttgttgcca tcgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9241 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9301 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9361 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9421 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9481 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9541 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9601 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9661 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9721 aagggcgaca cggaaatgtt gaatactcat attcttcctt
//
