LOCUS       sequence-304231-        9766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-ROBO3-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5371..5406
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5416..5439
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7099..7143
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7144..7170
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7310..7327)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7316..7427
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7545..7565)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7550..7879
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7730..7865
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7792..7811
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7927..7974
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7993..8592
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7993..8012)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8489..8509
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8722..8843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8759..8778)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8813..8832
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8896..9687,9688..9756))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9519..9538
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9757..9766,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccgg cgatatcagc
     2761 aattcttctg aactgctgct gggcttcaac agcagcctgg ccgctctgaa tcacaccttg
     2821 cttccacctg gcgaccccag cctgaatgga agtagagtgg gacccgagga cgccatgcct
     2881 agaatcgttg agcagcctcc tgacctgctg gtgtccagag gcgaacctgc tacactgcct
     2941 tgcagagccg aaggcagacc cagacctaac atcgagtggt acaagaacgg cgccagagtg
     3001 gccacagtgc gcgaagatcc tagagcacat aggctgctgc tgcctagcgg agccctgttc
     3061 ttcccaagaa tcgtgcatgg cagacgggcc agacctgacg aaggcgtgta cacttgcgtg
     3121 gccagaaact atctgggcgc tgccgcttcc agaaacgcct ctctggaagt ggccgtgctg
     3181 cgggacgatt tcagacagtc tcctggcaat gtggtggtgg ccgtgggaga acctgctgtg
     3241 ctggaatgtg tgcctcctag aggacaccct gagccttccg tgtcttggag aaaggatggc
     3301 gccagactga aagaggaaga gggcagaatc accatcagag gcggcaagct gatgatgagc
     3361 cacacactga agtccgacgc cgggatgtac gtgtgtgtgg cctctaatat ggccggcgag
     3421 agagaatctg ccgccgctga agtgatggtg ctggaaagac ccagcttcct gagaaggccc
     3481 gtgaaccagg ttgtgctggc tgatgcccct gtgaccttcc tgtgtgaagt gaagggcgac
     3541 cctccaccta gactgagatg gcggaaagag gatggcgagc tgcctaccgg cagatacgag
     3601 atcagatccg accactctct gtggatcggc catgtgtctg ccgaggacga gggcacatat
     3661 acctgtgtgg ccgagaactc tgtggggaga gctgaggctt ctggcagcct gtctgtgcat
     3721 gtgcctccac agctggtcac ccagcctcag gatcaaatgg ctgcccctgg cgagtctgtg
     3781 gcctttcagt gtgaaacaaa gggcaatcct cctcctgcca tcttttggca gaaagagggc
     3841 tcccaggtcc tgctgttccc tagccaatct ctgcagccca ccggccgctt ttctgtgtca
     3901 ccaagaggcc agctgaacat caccgctgtg caaagaggcg acgccggcta ctatgtgtgt
     3961 caggctgttt ctgtggccgg cagcattctg gccaaggctc tgctggaaat caagggcgct
     4021 tccctggatg gactgcctcc agtgattctt cagggcccag ccaatcagac actggtgctg
     4081 ggaagtagcg tgtggctgcc ctgtagagtg accggcaatc cacagcctag cgtgcggtgg
     4141 aagaaagacg gacaatggct gcagggcgac gacctgcagt ttaagaccat ggccaacggc
     4201 accctgtata tcgccaacgt gcaagagatg gacatggggt tctactcttg tgtggccaag
     4261 agcagcacag gcgaggctac ttggagcggc tggctgaaga tgagagagga ttggggcgtc
     4321 agccccgatc ctcctacaga gcctagtagt cctcctggcg ctccttctca gcctgtggtc
     4381 accgagatca ccaagaacag catcaccctg acctggaagc ctaatcctca gacaggcgcc
     4441 gctgtgacct cctacgtgat cgaggctttt tctcccgccg ctggcaatac ctggcggaca
     4501 gttgctgatg gcgtgcagct ggaaacccac accgtgtctg gactgcagcc taacaccatc
     4561 tacctgttcc tcgtcagagc cgttggagcc tggggactga gtgaaccctc tcctgtgtcc
     4621 gaacctgtgc ggacccaaga cagctctcct agcagacccg tggaagatcc ttggagagga
     4681 cagcagggac ttgccgaggt ggccgttaga ctgcaagagc caattgtgct gggacccaga
     4741 acactccagg tgtcctggac agtggatggc cctgtgcaac tggtgcaggg ctttagagtc
     4801 tcttggagag tggctggacc tgaaggcggc tcttggacca tgctggatct gcagtctcca
     4861 agccagcagt ccaccgtgct tagaggactg ccacctggca cacagatcca gatcaaggtg
     4921 caggcccagg gccaagaagg actgggagct gaatctctga gcgtgacccg cagcatccca
     4981 gaggaagctc catctggacc tccacagggt gttgccgttg cacttggcgg agatggcaac
     5041 agctctatca ccgtgtcctg ggaacctcca ctgcctagcc agcagaatgg cgtgatcacc
     5101 gagtatcaga tctggtgcct gggcaacgag agccggttcc acctgaatag atctgccgct
     5161 ggatgggcca gatccgccat gctgagagga cttgttcccg gcctgctgta cagaacactg
     5221 gtggccgctg ctacatctgc tggcgtggga gttccttctg ctcctgtgct ggttcagctg
     5281 ccttctcctc ctgatctgga acccggcctg gaagttggag ctggccttgc tgttagactg
     5341 gcccgggttc tgagagaacc tggcgcgccg gaagatcaag ttgatccaag attgattgat
     5401 ggcaagggcg gatctctgga ggtgctgttc cagggaccag gcggaagcgg caatcccggc
     5461 ggcgatctgg ccccccagat gctgcgcgag ctgcaggaga ccaatgccgc cctgcaggac
     5521 gtgcgcgagc tgctgcgcca ccgcgtgaag gagatcacct tcctgaagaa caccgtgatg
     5581 gagtgcgacg cctgcggtat gcagccctct agaggctccg gctccatcat cccagttgag
     5641 gaggagaacc cggacttctg gaaccgcgag gcagccgagg ccctgggtgc cgccaagaag
     5701 ctgcagcctg cacagacagc cgccaagaac ctcatcatct tcctgggcga tgggatgggg
     5761 gtgtctacgg tgacagctgc caggatccta aaagggcaga agaaggacaa actggggcct
     5821 gagatacccc tggccatgga ccgcttccca tatgtggctc tgtccaagac atacaatgta
     5881 gacaaacatg tgccagacag tggagccaca gccacggcct acctgtgcgg ggtcaagggc
     5941 aacttccaga ccattggctt gagtgcagcc gcccgcttta accagtgcaa cacgacacgc
     6001 ggcaacgagg tcatctccgt gatgaatcgg gccaagaaag cagggaagtc agtgggagtg
     6061 gtaaccacca cacgagtgca gcacgcctcg ccagccggca cctacgccca cacggtgaac
     6121 cgcaactggt actcggacgc cgacgtgcct gcctcggccc gccaggaggg gtgccaggac
     6181 atcgctacgc agctcatctc caacatggac attgacgtga tcctaggtgg aggccgaaag
     6241 tacatgtttc gcatgggaac cccagaccct gagtacccag atgactacag ccaaggtggg
     6301 accaggctgg acgggaagaa tctggtgcag gaatggctgg cgaagcgcca gggtgcccgg
     6361 tatgtgtgga accgcactga gctcatgcag gcttccctgg acccgtctgt gacccatctc
     6421 atgggtctct ttgagcctgg agacatgaaa tacgagatcc accgagactc cacactggac
     6481 ccctccctga tggagatgac agaggctgcc ctgcgcctgc tgagcaggaa cccccgcggc
     6541 ttcttcctct tcgtggaggg tggtcgcatc gaccatggtc atcatgaaag cagggcttac
     6601 cgggcactga ctgagacgat catgttcgac gacgccattg agagggcggg ccagctcacc
     6661 agcgaggagg acacgctgag cctcgtcact gccgaccact cccacgtctt ctccttcgga
     6721 ggctaccccc tgcgagggag ctccatcttc gggctggccc ctggcaaggc ccgggacagg
     6781 aaggcctaca cggtcctcct atacggaaac ggtccaggct atgtgctcaa ggacggcgcc
     6841 cggccggatg ttaccgagag cgagagcggg agccccgagt atcggcagca gtcagcagtg
     6901 cccctggacg aagagaccca cgcaggcgag gacgtggcgg tgttcgcgcg cggcccgcag
     6961 gcgcacctgg ttcacggcgt gcaggagcag accttcatag cgcacgtcat ggccttcgcc
     7021 gcctgcctgg agccctacac cgcctgcgac ctggcgcccc ccgccggcac caccgacgcc
     7081 gcgcacccgg gtggcggagg actgaacgac atcttcgagg ctcagaaaat cgaatggcac
     7141 gaacatcacc atcaccatca ccatcaccat taaggccggc ctgtagtcac tggcacaacg
     7201 cgttacccgg taagccaatc gggtatacac ggtcgtcata ctgcagacag ggttcttcta
     7261 ctttgcaaga tagtcttgag tagtaaaata aatagataga gaaaatcagc ctcgactgtg
     7321 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7381 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcaca acactcaacc
     7441 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7501 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     7561 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7621 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7681 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7741 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7801 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     7861 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     7921 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     7981 aggaactaaa ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     8041 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     8101 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     8161 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     8221 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     8281 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     8341 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     8401 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     8461 cccgccttcc tggagacatc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     8521 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     8581 cccggtgcct gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     8641 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8701 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     8761 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8821 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     8881 gcgtaatcat ggtcattacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8941 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9001 cttaccatct ggccccagcg ctgcgatgat accgcgagaa ccacgctcac cggctccgga
     9061 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9121 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9181 taatagtttg cgcaacgttg ttgccatcgc tacaggcatc gtggtgtcac gctcgtcgtt
     9241 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9301 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9361 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9421 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9481 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9541 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9601 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9661 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9721 gggaataagg gcgacacgga aatgttgaat actcatattc ttcctt
//
