LOCUS       sequence-304237-        9781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-SDK2(Trunc2)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5386..5421
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5431..5454
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7114..7158
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7159..7185
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7325..7342)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7331..7442
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7560..7580)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7565..7894
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7745..7880
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7807..7826
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7942..7989
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8008..8607
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8008..8027)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8504..8524
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8737..8858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8774..8793)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8828..8847
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8911..9702,9703..9771))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9534..9553
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9772..9781,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccca agatgatgtg
     2761 tccccttact ttaagaccga gcctgtgcgg acccaggtcc accttgaggg aaatagactg
     2821 gtgctgacct gcatggccga aggctcttgg cctctggaat tcaagtggct gcacaacaac
     2881 agagagctga ccaagttcag cctggaatac cggtacatga tcaccagcct ggatagaacc
     2941 cacgccggct tctacagatg catcgtgcgg aatagaatgg gcgccctgct gcagagacag
     3001 acagaggtgc aagtggccta catgggcagc ttcgaggaag gcgagaaaca ccagtctgtg
     3061 tctcatggcg aggccgccgt gatcagagca cctagaattg ccagctttcc ccagcctcaa
     3121 gtgacctggt tcagagatgg ccggaagatc cctcctagca gcagaatcgc catcacactg
     3181 gaaaacaccc tggtcatcct gagcacagtg gcccctgatg ccggcagata ttacgtgcag
     3241 gccgtgaacg acaagaacgg cgacaacaag accagccagc ctatcacact gaccgtggaa
     3301 aatgttggcg gccctgccga tcctatcgct cccacaatca tcatccctcc aaagaacaca
     3361 agcgtggtgg ccggcacaag cgaagtgaca ctggaatgcg tggccaacgc cagacctctg
     3421 atcaagctgc acatcatttg gaagaaagac ggcgtgctgc tgagcggcgg catcagcgat
     3481 cataacagac ggctgacaat ccccaatcct acaggctccg atgccggcta ctatgagtgt
     3541 gaagccgtgc tgagaagcag cagcgtgcca tctgttgtca gaggcgccta cctgtctgtg
     3601 ctggaacctc ctcagttcgt gaaagagccc gagagacaca tcaccgccga gatggaaaag
     3661 gtggtggata tcccttgtca ggccaagggc gtgccaccac ctagcatcac atggtacaag
     3721 gacgccgccg tggtggaagt ggaaaagctg accagattcc ggcagagaaa cgatggcggc
     3781 ctgcagattt ctggcctggt gcctgatgat accggcatgt tccagtgctt cgccagaaat
     3841 gcagccggcg aggtgcagac aagcacatac ctggccgtga cctctatcgc ccctaacatc
     3901 acaagaggcc ctctggacag caccgtgatc gatggaatgt ctgtggtgct ggcctgtgaa
     3961 acctctggcg cccctagacc tgccattacc tggcagaagg gcgagagaat tctggccagt
     4021 ggaagcgtgc agctgcctag attcacacct ctggaaagcg gcagcctgct gatctcccct
     4081 acacacatct ctgacgccgg cacctacacc tgtctggcca caaatagtag aggcgtggac
     4141 gaagccagcg ccgatctggt tgtttgggcc agaaccagaa tcaccaagcc tccacaggac
     4201 cagagcgtga tcaagggcac acaggcctct atggtttgcg gcgtgacaca cgaccctaga
     4261 gtgaccatcc ggtacatctg ggagaaagat ggggccacac tgggcaccga gtctcaccct
     4321 agaatcagac tggacagaaa cggctccctg cacatctctc agacttggag cggcgacatc
     4381 ggcacataca catgcagagt gatcagcgct ggcggcaacg acagtagatc tgcccacctg
     4441 agagtcagac agctgcctca cgctcctgaa caccctgtgg ctaccctgtc cacagtggaa
     4501 agaagggcca tcaacctgac ctggaccaag cctttcgacg gcaatagccc tctgatccgc
     4561 tacatcctgg aaatgagcga gaacaacgcc ccttggacag tgctgctggc ctctgtggac
     4621 cctaaggcca cctctgtgac agtgaaagga ctggtgccag ccagaagcta ccagttcaga
     4681 ctgtgcgccg tgaatgatgt cggcaagggc cagttcagca aggacaccga aagagtgtcc
     4741 ctgcctgagg aacctccaac cgctcctcct cagaatgtga tcgccagcgg caggaccaac
     4801 cagagcatca tgattcagtg gcagccacct cctgagagcc accagaatgg catcctgaag
     4861 ggctacatca tcagatactg cctggccgga ctgcccgtgg gctatcagtt caagaacatc
     4921 accgacgccg acgtgaacaa cctgctgctg gaagatctga tcatctggac caactacgag
     4981 atcgaggtgg ccgcctacaa ttctgccggc ctgggcgtgt acagcagcaa agtgacagaa
     5041 tggacactgc agggcgtccc aacagtgcct cctggcaatg tgcatgccga ggccaccaat
     5101 agcaccacca tcagattcac ctggaacgcc ccatctccac agttcatcaa cggcatcaac
     5161 cagggctaca agctgatcgc ctgggagcct gagcaagagg aagaggtcac aatggtcacc
     5221 gccaggccta acttccagga tagcatccac gtgggcttcg tgtccggcct gaagaagttc
     5281 accgagtact tcaccagcgt gctgtgcttc acaacacctg gcgacggccc tagatctaca
     5341 cctcagctcg tgcggacaca cgaggatgtt cccggtggcg cgccggaaga tcaagttgat
     5401 ccaagattga ttgatggcaa gggcggatct ctggaggtgc tgttccaggg accaggcgga
     5461 agcggcaatc ccggcggcga tctggccccc cagatgctgc gcgagctgca ggagaccaat
     5521 gccgccctgc aggacgtgcg cgagctgctg cgccaccgcg tgaaggagat caccttcctg
     5581 aagaacaccg tgatggagtg cgacgcctgc ggtatgcagc cctctagagg ctccggctcc
     5641 atcatcccag ttgaggagga gaacccggac ttctggaacc gcgaggcagc cgaggccctg
     5701 ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat catcttcctg
     5761 ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg gcagaagaag
     5821 gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt ggctctgtcc
     5881 aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac ggcctacctg
     5941 tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg ctttaaccag
     6001 tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa gaaagcaggg
     6061 aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc cggcacctac
     6121 gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc ggcccgccag
     6181 gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga cgtgatccta
     6241 ggtggaggcc gaaagtacat gtttcgcatg ggaaccccag accctgagta cccagatgac
     6301 tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg gctggcgaag
     6361 cgccagggtg cccggtatgt gtggaaccgc actgagctca tgcaggcttc cctggacccg
     6421 tctgtgaccc atctcatggg tctctttgag cctggagaca tgaaatacga gatccaccga
     6481 gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg cctgctgagc
     6541 aggaaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca tggtcatcat
     6601 gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc cattgagagg
     6661 gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga ccactcccac
     6721 gtcttctcct tcggaggcta ccccctgcga gggagctcca tcttcgggct ggcccctggc
     6781 aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc aggctatgtg
     6841 ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc cgagtatcgg
     6901 cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt ggcggtgttc
     6961 gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt catagcgcac
     7021 gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc gccccccgcc
     7081 ggcaccaccg acgccgcgca cccgggtggc ggaggactga acgacatctt cgaggctcag
     7141 aaaatcgaat ggcacgaaca tcaccatcac catcaccatc accattaagg ccggcctgta
     7201 gtcactggca caacgcgtta cccggtaagc caatcgggta tacacggtcg tcatactgca
     7261 gacagggttc ttctactttg caagatagtc ttgagtagta aaataaatag atagagaaaa
     7321 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     7381 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     7441 tcacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     7501 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     7561 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7621 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     7681 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     7741 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7801 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     7861 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     7921 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     7981 taatacgaca aggtgaggaa ctaaaccatg accgagtaca agcccacggt gcgcctcgcc
     8041 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     8101 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     8161 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     8221 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     8281 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     8341 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     8401 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     8461 gagcgcgccg gggtgcccgc cttcctggag acatccgcgc cccgcaacct ccccttctac
     8521 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     8581 tgcatgaccc gcaagcccgg tgcctgacac gtgctacgag atttcgattc caccgccgcc
     8641 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     8701 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     8761 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8821 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     8881 tctagctaga gcttggcgta atcatggtca ttaccaatgc ttaatcagtg aggcacctat
     8941 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9001 tacgatacgg gagggcttac catctggccc cagcgctgcg atgataccgc gagaaccacg
     9061 ctcaccggct ccggatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9121 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9181 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc atcgctacag gcatcgtggt
     9241 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9301 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9361 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9421 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9481 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9541 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9601 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9661 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9721 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tattcttcct
     9781 t
//
