LOCUS       sequence-304239-        9856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-MDGA2-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     protein_bind    3655..3669
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     CDS             5461..5496
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5506..5529
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7189..7233
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7234..7260
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7400..7417)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7406..7517
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7635..7655)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7640..7969
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7820..7955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8017..8064
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8083..8682
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8083..8102)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8579..8599
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8812..8933
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8849..8868)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8903..8922
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8986..9777,9778..9846))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9609..9628
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9847..9856,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccac agttagaatc
     2761 gtgcattctg gcctggcctg caacatcgag gaagaacggt acagcgagag agtgtacacc
     2821 atcagagagg gcgagacact ggaactgacc tgcctggtta ccggccatcc tcggcctcag
     2881 atcagatgga caaagacagc cggcagcgcc agcgacagat tccaggatag cagcgtgttc
     2941 aacgaaaccc tgcggatcac caacatccag cggcatcaag gcggacggta ctactgcaag
     3001 gccgagaatg gactgggcag ccctgccatc aagagcatca gagtggacgt gtactacctg
     3061 gacgaccccg tggttacagt gcaccagtct atcggcgagg ccaaagagca gttctactac
     3121 gagcggaccg tgttcctgag atgcgtggcc aacagcaatc ctcctgtgcg gtactcttgg
     3181 cggagaggcc aagaagttct gctgcagggc tctgacaaag gcgtggaaat ctacgagcca
     3241 ttcttcaccc aaggcgagac aaagatcctg aagctgaaga acctgcggcc tcaggactac
     3301 gccaactact cctgtattgc cagcgtgcgg aacgtgtgca acatccccga taagatggtg
     3361 tccttccggc tgagcaacaa gaccgcctct cctagcatca agctgctggt ggacgatccc
     3421 atcgtggtca atcctggcga ggctatcacc ctcgtgtgtg tgacaacagg cggagagcct
     3481 gctcctagcc tgacatgggt ccgaagcttt ggcaccctgc ctgagaaaac cgtgctgaat
     3541 ggcggcaccc tgacaatccc agccatcacc tctgatgacg ccggcaccta cagctgcatt
     3601 gccaacaaca acgtgggcaa ccccgccaag aaaagcacca atatcatcgt gcgggccctg
     3661 aagaagggca gattctggat cacccctgat ccttaccaca aggacgacaa tatccagatc
     3721 ggccgggaag tgaagatcag ctgccaggtg gaagccgtgc ctagcgagga actgacattc
     3781 agctggttca agaacggcag acccctgaga agcagcgagc ggatggtcat cacccagacc
     3841 gatcctgatg tgtcccctgg caccaccaac ctggacatca tcgacctgaa gttcaccgac
     3901 ttcggcacct atacctgtgt ggccagcctg aaaggcggcg gaatcagcga catcagcatc
     3961 gacgtgaaca tcagcagcag caccgtgcct ccaaacctga ccgtgccgca agagaagtct
     4021 cctctggtca caagagaagg cgacaccatc gagctgcagt gtcaagtgac cggcaagccc
     4081 aagcctatca tcctgtggtc cagagccgac aaagaggtgg ccatgccaga tggcagcatg
     4141 cagatggaaa gctacgacgg cacactgcgg atcgtgaacg tgtccagaga aatgagcggc
     4201 atgtaccggt gccagaccag ccagtacaac ggctttaacg tgaagccccg ggaagccctg
     4261 gtgcagctga ttgtgcaata tcctcctgcc gtggaacccg ccttcctgga aatcagacag
     4321 ggccaagacc gcagcgtgac catgagctgt agagtgctgc gggcctatcc tatccgggtg
     4381 ctgacatatg agtggcgcct gggcaacaaa ctgctgagaa ccggccagtt cgactctcaa
     4441 gagtacaccg agtacgccgt gaagtccctg agcaacgaga actacggcgt gtacaactgc
     4501 agcatcatca acgaggctgg cgccggaaga tgcagctttc tggtcaccgg aaaggcctac
     4561 gctcccgagt tttactacga cacctacaat cccgtgtggc agaaccggca ccgggtgtac
     4621 agttacagcc tgcagtggac ccagatgaac cccgacgccg tggataggat cgtggcctat
     4681 agactgggca ttagacaggc cggacagcag cgttggtggg agcaagagat caagatcaac
     4741 gggaacatcc agaagggcga gctgatcacc tacaacctga cagagctgat taagcccgag
     4801 gcctacgaag tgcggctgac ccctctgacc aagtttggcg agggcgacag caccattaga
     4861 gtgatcaagt acagcgcccc tgtgaaccct cacctgagag agttccactg cggcttcgag
     4921 gacggcaaca tctgcctgtt cacccaggac gacaccgaca acttcgactg gaccaagcag
     4981 agcaccgcca ccagaaacac caagtacacc cctaacacag gccccaacgc cgacagaagc
     5041 ggcagcaaag agggcttcta catgtacatc gagacaagca gaccccggct ggaaggcgag
     5101 aaagccagac tgctgagccc cgtgttttct atcgccccta agaaccctta cggccccacc
     5161 aataccgcct actgcttctc cttcttctac catatgtacg gccagcacat cggagtgctg
     5221 aacgtgtacc tgagactgaa gggccagaca accatcgaga accctctgtg gtctagcagc
     5281 ggcaacaaag gccagagatg gaacgaggcc catgtgaaca tctaccccat caccagcttc
     5341 cagctgattt tcgagggcat cagaggccct ggcatcgagg gcgatattgc catcgacgac
     5401 gtgtccattg ccgagggcga gtgtgccaaa caggatctgg ccaccaagaa tggcgcgccg
     5461 gaagatcaag ttgatccaag attgattgat ggcaagggcg gatctctgga ggtgctgttc
     5521 cagggaccag gcggaagcgg caatcccggc ggcgatctgg ccccccagat gctgcgcgag
     5581 ctgcaggaga ccaatgccgc cctgcaggac gtgcgcgagc tgctgcgcca ccgcgtgaag
     5641 gagatcacct tcctgaagaa caccgtgatg gagtgcgacg cctgcggtat gcagccctct
     5701 agaggctccg gctccatcat cccagttgag gaggagaacc cggacttctg gaaccgcgag
     5761 gcagccgagg ccctgggtgc cgccaagaag ctgcagcctg cacagacagc cgccaagaac
     5821 ctcatcatct tcctgggcga tgggatgggg gtgtctacgg tgacagctgc caggatccta
     5881 aaagggcaga agaaggacaa actggggcct gagatacccc tggccatgga ccgcttccca
     5941 tatgtggctc tgtccaagac atacaatgta gacaaacatg tgccagacag tggagccaca
     6001 gccacggcct acctgtgcgg ggtcaagggc aacttccaga ccattggctt gagtgcagcc
     6061 gcccgcttta accagtgcaa cacgacacgc ggcaacgagg tcatctccgt gatgaatcgg
     6121 gccaagaaag cagggaagtc agtgggagtg gtaaccacca cacgagtgca gcacgcctcg
     6181 ccagccggca cctacgccca cacggtgaac cgcaactggt actcggacgc cgacgtgcct
     6241 gcctcggccc gccaggaggg gtgccaggac atcgctacgc agctcatctc caacatggac
     6301 attgacgtga tcctaggtgg aggccgaaag tacatgtttc gcatgggaac cccagaccct
     6361 gagtacccag atgactacag ccaaggtggg accaggctgg acgggaagaa tctggtgcag
     6421 gaatggctgg cgaagcgcca gggtgcccgg tatgtgtgga accgcactga gctcatgcag
     6481 gcttccctgg acccgtctgt gacccatctc atgggtctct ttgagcctgg agacatgaaa
     6541 tacgagatcc accgagactc cacactggac ccctccctga tggagatgac agaggctgcc
     6601 ctgcgcctgc tgagcaggaa cccccgcggc ttcttcctct tcgtggaggg tggtcgcatc
     6661 gaccatggtc atcatgaaag cagggcttac cgggcactga ctgagacgat catgttcgac
     6721 gacgccattg agagggcggg ccagctcacc agcgaggagg acacgctgag cctcgtcact
     6781 gccgaccact cccacgtctt ctccttcgga ggctaccccc tgcgagggag ctccatcttc
     6841 gggctggccc ctggcaaggc ccgggacagg aaggcctaca cggtcctcct atacggaaac
     6901 ggtccaggct atgtgctcaa ggacggcgcc cggccggatg ttaccgagag cgagagcggg
     6961 agccccgagt atcggcagca gtcagcagtg cccctggacg aagagaccca cgcaggcgag
     7021 gacgtggcgg tgttcgcgcg cggcccgcag gcgcacctgg ttcacggcgt gcaggagcag
     7081 accttcatag cgcacgtcat ggccttcgcc gcctgcctgg agccctacac cgcctgcgac
     7141 ctggcgcccc ccgccggcac caccgacgcc gcgcacccgg gtggcggagg actgaacgac
     7201 atcttcgagg ctcagaaaat cgaatggcac gaacatcacc atcaccatca ccatcaccat
     7261 taaggccggc ctgtagtcac tggcacaacg cgttacccgg taagccaatc gggtatacac
     7321 ggtcgtcata ctgcagacag ggttcttcta ctttgcaaga tagtcttgag tagtaaaata
     7381 aatagataga gaaaatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     7441 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     7501 aatgaggaaa ttgcatcaca acactcaacc ctatctcggt ctattctttt gatttataag
     7561 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7621 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     7681 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7741 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7801 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7861 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     7921 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     7981 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     8041 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatgaccga gtacaagccc
     8101 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     8161 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     8221 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     8281 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     8341 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     8401 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     8461 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     8521 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacatc cgcgccccgc
     8581 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     8641 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacacgtgct acgagatttc
     8701 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     8761 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     8821 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8881 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8941 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcattacc aatgcttaat
     9001 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9061 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagcg ctgcgatgat
     9121 accgcgagaa ccacgctcac cggctccgga tttatcagca ataaaccagc cagccggaag
     9181 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9241 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccatcgc
     9301 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9361 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9421 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9481 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9541 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9601 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9661 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9721 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9781 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9841 actcatattc ttcctt
//
