LOCUS       sequence-304240-        2703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-MDGA2-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..2703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    907..921
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
ORIGIN
        1 acagttagaa tcgtgcattc tggcctggcc tgcaacatcg aggaagaacg gtacagcgag
       61 agagtgtaca ccatcagaga gggcgagaca ctggaactga cctgcctggt taccggccat
      121 cctcggcctc agatcagatg gacaaagaca gccggcagcg ccagcgacag attccaggat
      181 agcagcgtgt tcaacgaaac cctgcggatc accaacatcc agcggcatca aggcggacgg
      241 tactactgca aggccgagaa tggactgggc agccctgcca tcaagagcat cagagtggac
      301 gtgtactacc tggacgaccc cgtggttaca gtgcaccagt ctatcggcga ggccaaagag
      361 cagttctact acgagcggac cgtgttcctg agatgcgtgg ccaacagcaa tcctcctgtg
      421 cggtactctt ggcggagagg ccaagaagtt ctgctgcagg gctctgacaa aggcgtggaa
      481 atctacgagc cattcttcac ccaaggcgag acaaagatcc tgaagctgaa gaacctgcgg
      541 cctcaggact acgccaacta ctcctgtatt gccagcgtgc ggaacgtgtg caacatcccc
      601 gataagatgg tgtccttccg gctgagcaac aagaccgcct ctcctagcat caagctgctg
      661 gtggacgatc ccatcgtggt caatcctggc gaggctatca ccctcgtgtg tgtgacaaca
      721 ggcggagagc ctgctcctag cctgacatgg gtccgaagct ttggcaccct gcctgagaaa
      781 accgtgctga atggcggcac cctgacaatc ccagccatca cctctgatga cgccggcacc
      841 tacagctgca ttgccaacaa caacgtgggc aaccccgcca agaaaagcac caatatcatc
      901 gtgcgggccc tgaagaaggg cagattctgg atcacccctg atccttacca caaggacgac
      961 aatatccaga tcggccggga agtgaagatc agctgccagg tggaagccgt gcctagcgag
     1021 gaactgacat tcagctggtt caagaacggc agacccctga gaagcagcga gcggatggtc
     1081 atcacccaga ccgatcctga tgtgtcccct ggcaccacca acctggacat catcgacctg
     1141 aagttcaccg acttcggcac ctatacctgt gtggccagcc tgaaaggcgg cggaatcagc
     1201 gacatcagca tcgacgtgaa catcagcagc agcaccgtgc ctccaaacct gaccgtgccg
     1261 caagagaagt ctcctctggt cacaagagaa ggcgacacca tcgagctgca gtgtcaagtg
     1321 accggcaagc ccaagcctat catcctgtgg tccagagccg acaaagaggt ggccatgcca
     1381 gatggcagca tgcagatgga aagctacgac ggcacactgc ggatcgtgaa cgtgtccaga
     1441 gaaatgagcg gcatgtaccg gtgccagacc agccagtaca acggctttaa cgtgaagccc
     1501 cgggaagccc tggtgcagct gattgtgcaa tatcctcctg ccgtggaacc cgccttcctg
     1561 gaaatcagac agggccaaga ccgcagcgtg accatgagct gtagagtgct gcgggcctat
     1621 cctatccggg tgctgacata tgagtggcgc ctgggcaaca aactgctgag aaccggccag
     1681 ttcgactctc aagagtacac cgagtacgcc gtgaagtccc tgagcaacga gaactacggc
     1741 gtgtacaact gcagcatcat caacgaggct ggcgccggaa gatgcagctt tctggtcacc
     1801 ggaaaggcct acgctcccga gttttactac gacacctaca atcccgtgtg gcagaaccgg
     1861 caccgggtgt acagttacag cctgcagtgg acccagatga accccgacgc cgtggatagg
     1921 atcgtggcct atagactggg cattagacag gccggacagc agcgttggtg ggagcaagag
     1981 atcaagatca acgggaacat ccagaagggc gagctgatca cctacaacct gacagagctg
     2041 attaagcccg aggcctacga agtgcggctg acccctctga ccaagtttgg cgagggcgac
     2101 agcaccatta gagtgatcaa gtacagcgcc cctgtgaacc ctcacctgag agagttccac
     2161 tgcggcttcg aggacggcaa catctgcctg ttcacccagg acgacaccga caacttcgac
     2221 tggaccaagc agagcaccgc caccagaaac accaagtaca cccctaacac aggccccaac
     2281 gccgacagaa gcggcagcaa agagggcttc tacatgtaca tcgagacaag cagaccccgg
     2341 ctggaaggcg agaaagccag actgctgagc cccgtgtttt ctatcgcccc taagaaccct
     2401 tacggcccca ccaataccgc ctactgcttc tccttcttct accatatgta cggccagcac
     2461 atcggagtgc tgaacgtgta cctgagactg aagggccaga caaccatcga gaaccctctg
     2521 tggtctagca gcggcaacaa aggccagaga tggaacgagg cccatgtgaa catctacccc
     2581 atcaccagct tccagctgat tttcgagggc atcagaggcc ctggcatcga gggcgatatt
     2641 gccatcgacg acgtgtccat tgccgagggc gagtgtgcca aacaggatct ggccaccaag
     2701 aat
//
