LOCUS       sequence-304243-        9886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-MDGA1-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5491..5526
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5536..5559
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7219..7263
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7264..7290
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7430..7447)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7436..7547
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7665..7685)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7670..7999
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7850..7985
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7912..7931
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8047..8094
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8113..8712
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8113..8132)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8609..8629
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8842..8963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8879..8898)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8933..8952
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9016..9807,9808..9876))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9639..9658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9877..9886,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccca aggcgtttac
     2761 gctcctgctc aagctcagat tgtgcatgcc ggccaggcct gcgtggtcaa agaggacaat
     2821 atcagcgagc gcgtgtacac catcagagaa ggcgataccc tgatgctgca gtgcctggtc
     2881 acaggacacc ctagacctca agtgcggtgg acaaaaacag ccggcagcgc cagcgacaag
     2941 ttccaagaga caagcgtgtt caacgagaca ctgcggatcg agcggatcgc cagaacacaa
     3001 ggcggcagat attactgcaa ggccgagaat ggcgtgggcg tgccagccat caagagcatc
     3061 agagtggacg tgcagtacct ggacgagcct atgctgacag tgcaccagac agtgtccgac
     3121 gtgcggggca acttctacca agaaaagacc gtgttcctga ggtgcaccgt gaacagcaac
     3181 cctccagcca gattcatctg gaagagaggc agcgacacac tgagccacag ccaggataac
     3241 ggcgtggaca tctacgagcc tctgtacaca cagggcgaga caaaggtgct gaagctgaag
     3301 aatctgcggc cccaggacta cgccagctat acttgtcagg tgtccgtgcg gaatgtgtgc
     3361 ggcatccctg ataaggccat caccttccgg ctgaccaaca caacagcccc tccagctctg
     3421 aagctgtccg tgaatgagac actggtggtc aaccccggcg agaatgtgac tgtgcaatgt
     3481 ctgctgacag gcggcgatcc tctgcctcag cttcaatggt cacatggccc tggacctttg
     3541 cctctgggag cacttgctca aggcggaaca ctgtctattc ccagcgtgca ggccagagac
     3601 agcggctact ataattgcac cgccaccaac aacgtgggca accccgccaa gaaaaccgtg
     3661 aatctgctcg tgcggagcat gaagaacgcc accttccaga tcaccccaga cgtgatcaaa
     3721 gagagcgaga acatccagct cggccaggac ctgaaactga gctgccatgt ggatgccgtg
     3781 cctcaagaga aagtgaccta ccagtggttc aagaacggca agcccgccag aatgagcaag
     3841 agactgctgg tcaccagaaa cgatccagag ctgcctgccg tgacaagcag cctggaactg
     3901 atcgacctgc acttcagcga ctacggcacc tacctgtgca tggctagttt tccaggcgct
     3961 cccgtgcctg atctgagcgt ggaagtgaac atcagcagcg agacagtgcc tcctaccatc
     4021 agcgtgccaa aaggcagagc cgtcgtgaca gttagagagg gctctcctgc cgaactgcag
     4081 tgtgaagtgc ggggaaaacc cagacctcct gtgctgtggt cccgcgtgga caaagaagct
     4141 gctctgctgc cttctggact gcccctggaa gaaacacccg atggcaagct gagactggaa
     4201 cgggtgtcca gagacatgag cggcacatac agatgccaga ccgccagata caacggcttc
     4261 aacgtgcggc ctagagaagc ccaggtgcag ctgaatgtgc agttccctcc agaggtggaa
     4321 cctagctctc aggatgtgcg acaagctctg ggtagacccg tgctgctcag atgttctctg
     4381 ctgagaggct cccctcagag aattgcctct gccgtgtggc ggtttaaggg acaacttctg
     4441 cctcctcctc ctgtggtgcc tgctgctgct gaagcacctg atcacgctga actgaggctg
     4501 gacgccgtga ccagagatag cagcggcagc tacgagtgca gcgtgtccaa tgatgtggga
     4561 agcgccgcct gcctgtttca agtgtctgcc aaggcctact ctcccgagtt ctacttcgac
     4621 acccctaatc ctaccagaag ccacaagctg agcaagaact acagctacgt cctgcagtgg
     4681 acccagaggg aacctgatgc agtcgacccc gtgctgaact acagactgtc catcagacag
     4741 ctgaaccagc acaacgccgt ggtcaaggct atccctgtgc ggagagtgga aaagggccag
     4801 ctgctcgagt acatcctgac cgatctgaga gtgccccact cctacgaagt gcggctgacc
     4861 ccttacacaa ccttcggagc tggcgacatg gccagccgga tcatccacta caccgagcct
     4921 atcaacagcc ctaacctgag cgacaatacc tgccacttcg aggacgagaa gatctgtggc
     4981 tacacccagg atctgaccga caacttcgac tggaccagac agaacgccct gacacagaac
     5041 cccaagcgga gccctaatac cggacctcct accgatatct ctggcacccc tgagggctac
     5101 tacatgttca tcgaaaccag cagacccaga gagctgggcg atagagctag actggtgtcc
     5161 ccactgtaca acgcctccgc caagttctac tgcgtgtcct tcttctacca tatgtacggc
     5221 aagcacatcg gcagcctgaa cctgcttgtg cggtcaagaa acaaaggcgc cctggataca
     5281 cacgcttgga gcctgtctgg caacaagggc aatgtgtggc agcaggccca tgtgcctatc
     5341 agcccatctg gcccctttca gatcatcttc gagggcgtca gaggccctgg ctatctggga
     5401 gatatcgcca tcgacgacgt gaccctgaag aaaggcgagt gccctagaaa gcagacagac
     5461 cccaacaagg tggtggtcat gggcgcgccg gaagatcaag ttgatccaag attgattgat
     5521 ggcaagggcg gatctctgga ggtgctgttc cagggaccag gcggaagcgg caatcccggc
     5581 ggcgatctgg ccccccagat gctgcgcgag ctgcaggaga ccaatgccgc cctgcaggac
     5641 gtgcgcgagc tgctgcgcca ccgcgtgaag gagatcacct tcctgaagaa caccgtgatg
     5701 gagtgcgacg cctgcggtat gcagccctct agaggctccg gctccatcat cccagttgag
     5761 gaggagaacc cggacttctg gaaccgcgag gcagccgagg ccctgggtgc cgccaagaag
     5821 ctgcagcctg cacagacagc cgccaagaac ctcatcatct tcctgggcga tgggatgggg
     5881 gtgtctacgg tgacagctgc caggatccta aaagggcaga agaaggacaa actggggcct
     5941 gagatacccc tggccatgga ccgcttccca tatgtggctc tgtccaagac atacaatgta
     6001 gacaaacatg tgccagacag tggagccaca gccacggcct acctgtgcgg ggtcaagggc
     6061 aacttccaga ccattggctt gagtgcagcc gcccgcttta accagtgcaa cacgacacgc
     6121 ggcaacgagg tcatctccgt gatgaatcgg gccaagaaag cagggaagtc agtgggagtg
     6181 gtaaccacca cacgagtgca gcacgcctcg ccagccggca cctacgccca cacggtgaac
     6241 cgcaactggt actcggacgc cgacgtgcct gcctcggccc gccaggaggg gtgccaggac
     6301 atcgctacgc agctcatctc caacatggac attgacgtga tcctaggtgg aggccgaaag
     6361 tacatgtttc gcatgggaac cccagaccct gagtacccag atgactacag ccaaggtggg
     6421 accaggctgg acgggaagaa tctggtgcag gaatggctgg cgaagcgcca gggtgcccgg
     6481 tatgtgtgga accgcactga gctcatgcag gcttccctgg acccgtctgt gacccatctc
     6541 atgggtctct ttgagcctgg agacatgaaa tacgagatcc accgagactc cacactggac
     6601 ccctccctga tggagatgac agaggctgcc ctgcgcctgc tgagcaggaa cccccgcggc
     6661 ttcttcctct tcgtggaggg tggtcgcatc gaccatggtc atcatgaaag cagggcttac
     6721 cgggcactga ctgagacgat catgttcgac gacgccattg agagggcggg ccagctcacc
     6781 agcgaggagg acacgctgag cctcgtcact gccgaccact cccacgtctt ctccttcgga
     6841 ggctaccccc tgcgagggag ctccatcttc gggctggccc ctggcaaggc ccgggacagg
     6901 aaggcctaca cggtcctcct atacggaaac ggtccaggct atgtgctcaa ggacggcgcc
     6961 cggccggatg ttaccgagag cgagagcggg agccccgagt atcggcagca gtcagcagtg
     7021 cccctggacg aagagaccca cgcaggcgag gacgtggcgg tgttcgcgcg cggcccgcag
     7081 gcgcacctgg ttcacggcgt gcaggagcag accttcatag cgcacgtcat ggccttcgcc
     7141 gcctgcctgg agccctacac cgcctgcgac ctggcgcccc ccgccggcac caccgacgcc
     7201 gcgcacccgg gtggcggagg actgaacgac atcttcgagg ctcagaaaat cgaatggcac
     7261 gaacatcacc atcaccatca ccatcaccat taaggccggc ctgtagtcac tggcacaacg
     7321 cgttacccgg taagccaatc gggtatacac ggtcgtcata ctgcagacag ggttcttcta
     7381 ctttgcaaga tagtcttgag tagtaaaata aatagataga gaaaatcagc ctcgactgtg
     7441 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7501 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcaca acactcaacc
     7561 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7621 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     7681 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7741 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7801 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7861 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7921 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     7981 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     8041 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     8101 aggaactaaa ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     8161 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     8221 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     8281 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     8341 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     8401 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     8461 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     8521 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     8581 cccgccttcc tggagacatc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     8641 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     8701 cccggtgcct gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     8761 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8821 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     8881 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8941 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     9001 gcgtaatcat ggtcattacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9061 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9121 cttaccatct ggccccagcg ctgcgatgat accgcgagaa ccacgctcac cggctccgga
     9181 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9241 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9301 taatagtttg cgcaacgttg ttgccatcgc tacaggcatc gtggtgtcac gctcgtcgtt
     9361 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9421 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9481 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9541 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9601 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9661 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9721 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9781 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9841 gggaataagg gcgacacgga aatgttgaat actcatattc ttcctt
//
