LOCUS       sequence-304245-        9895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PRTG-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5500..5535
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5545..5568
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7228..7272
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7273..7299
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7439..7456)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7445..7556
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7674..7694)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7679..8008
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7859..7994
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7921..7940
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8056..8103
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8122..8721
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8122..8141)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8618..8638
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8851..8972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8888..8907)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8942..8961
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9025..9816,9817..9885))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9648..9667
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9886..9895,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgcctt tagcgagctg
     2761 agcttcgtga aagaacccca ggacgtgacc gtgaccagaa aggaccctgt ggtgctggat
     2821 tgtcaggccc acggcgaagt gcctatcaaa gtgacctggc tgaagaacgg cgccaagatg
     2881 agcgagaaca agcggatcga ggtgctgagc aacggcagcc tgtacatctc tgaggtggaa
     2941 ggcagaagag gcgagcagag cgacgagggc ttttaccagt gcctggccat gaacaaatac
     3001 ggcgccatcc tgagccagaa ggcccatctg gccctgagca caatctctgc ctttgaggtg
     3061 cagcccatca gcacagaagt gcacgaaggc ggagtggcca gattcgcctg caagatctct
     3121 tctcaccctc ctgccgtgat cacctgggag ttcaacagaa ccacactgcc catgaccatg
     3181 gaccggatca cagctttgcc aaccggcgtg ctgcagatct acgacgtgtc ccagagagac
     3241 agcggcaact acagatgtat cgccgccaca gtggcccaca gacggaagtc tatggaagcc
     3301 agcctgacag tgatccccgc caaagagagc aagagctttc acacccctac catcattgcc
     3361 ggacctcaga acatcaccac cagcctgcat cagaccgtgg tgctcgagtg tatggccacc
     3421 ggcaatccca agcctatcat cagctggtcc cggctggacc acaagagcat cgacgtgttc
     3481 aataccagag tgctcggcaa cggcaatctg atgatctccg atgtgcgact gcagcatgca
     3541 ggcgtgtacg tgtgtagagc caccacacct ggcaccagaa acttcaccgt ggccatggct
     3601 acactgacag tgctggcccc tcctagcttt gtggaatggc ctgagagcct gacaagacct
     3661 agagccggca ccgccagatt tgtgtgtcag gccgagggaa tcccatctcc taagatgagc
     3721 tggctgaaaa atggccggaa gatccacagc aacggccgga tcaagatgta caacagcaag
     3781 ctggtcatca accagatcat ccccgaggac gacgccatct atcagtgcat ggccgagaat
     3841 agccagggct ctatcctgag cagagccaga ctgaccgtgg tcatgagcga ggatagaccc
     3901 agcgctcctt acaacgtgca cgccgagaca atgagcagct ctgccattct gctggcctgg
     3961 gaaagacccc tgtacaactc cgacaaagtg atcgcctaca gcgtgcacta catgaaggcc
     4021 gaaggcctga acaacgagga ataccaggtg gtcatcggca acgacaccac acactacatc
     4081 atcgacgacc tggaacctgc cagcaactac accttctaca tcgtggccta catgcctatg
     4141 ggcgccagcc agatgtctga ccacgtgaca cagaacaccc tggaagatgt gcctctgcgg
     4201 cctcctgaga tcagcctgac tagcagaagc cccaccgaca tcctgatcag ctggctgcct
     4261 attcctgcca agtatcggag aggccaggtg gtgctgtaca gactgagctt cagactgtcc
     4321 accgagaaca gcatccaggt gctggaactg cctggcacaa cacacgagta cctgctggaa
     4381 ggactgaagc ccgattctgt gtacctcgtg cggattacag ccgccacaag agttggactg
     4441 ggagagtcta gcgtgtggac cagccataga acccctaagg ccacaagcgt gaaggcccct
     4501 aagtctcccg aactgcatct ggaacccctg aactgcacaa ccatctctgt gcggtggcag
     4561 caggatgtgg aagatacagc cgctatccag ggctacaagc tgtactacaa agaggaaggc
     4621 cagcaagaga acggccccat cttcctggat accaaggacc tgctgtacac cctgagcgga
     4681 ctggacccta gaagaaagta tcatgtgcgg ctgctggctt acaacaacat cgacgatggc
     4741 taccaggccg accagacagt gtctacacca ggatgcgtgt ccgtgcggga tagaatggtt
     4801 cctcctccac cacctccaca ccacctgtat gccaaggcca ataccagcag cagcatcttt
     4861 ctgcattggc ggaggcctgc ctttaccgcc gctcagatca tcaattacac catcagatgc
     4921 aaccctgtgg gcctgcagaa tgccagcctg gtcctgtatc tgcagaccag cgaaacccac
     4981 atgctggtgc agggactcga gcccaacacc aaatatgagt tcgccgtcag actgcatgtg
     5041 gatcagctgt ctagtccttg gagccccgtg gtgtaccact ctacattgcc tgaagctcca
     5101 gctggccctc ctgtgggagt gaaagtgacc ctgatcgagg acgatacagc cctggtgtcc
     5161 tggaagcctc ctgatggacc tgagacagtg gtcacccggt acacaatcct gtacgcctcc
     5221 agaaaggcct ggatcgctgg cgaatggcag gtcttgcata gagaaggcgc catcaccatg
     5281 gcactgctgg aaaatctggt ggccggcaac gtgtacatcg tgaagatcag cgccagcaac
     5341 gaagtcggcg agggcccttt tagcaatagc gtggaactgg ccgtgctgcc caaagaaacc
     5401 agcgagagca accagcggcc taagagactg gattctgccg acgccaaggt gtactccggc
     5461 tactaccacc tggatcagaa atccatgaca ggcgcgccgg aagatcaagt tgatccaaga
     5521 ttgattgatg gcaagggcgg atctctggag gtgctgttcc agggaccagg cggaagcggc
     5581 aatcccggcg gcgatctggc cccccagatg ctgcgcgagc tgcaggagac caatgccgcc
     5641 ctgcaggacg tgcgcgagct gctgcgccac cgcgtgaagg agatcacctt cctgaagaac
     5701 accgtgatgg agtgcgacgc ctgcggtatg cagccctcta gaggctccgg ctccatcatc
     5761 ccagttgagg aggagaaccc ggacttctgg aaccgcgagg cagccgaggc cctgggtgcc
     5821 gccaagaagc tgcagcctgc acagacagcc gccaagaacc tcatcatctt cctgggcgat
     5881 gggatggggg tgtctacggt gacagctgcc aggatcctaa aagggcagaa gaaggacaaa
     5941 ctggggcctg agatacccct ggccatggac cgcttcccat atgtggctct gtccaagaca
     6001 tacaatgtag acaaacatgt gccagacagt ggagccacag ccacggccta cctgtgcggg
     6061 gtcaagggca acttccagac cattggcttg agtgcagccg cccgctttaa ccagtgcaac
     6121 acgacacgcg gcaacgaggt catctccgtg atgaatcggg ccaagaaagc agggaagtca
     6181 gtgggagtgg taaccaccac acgagtgcag cacgcctcgc cagccggcac ctacgcccac
     6241 acggtgaacc gcaactggta ctcggacgcc gacgtgcctg cctcggcccg ccaggagggg
     6301 tgccaggaca tcgctacgca gctcatctcc aacatggaca ttgacgtgat cctaggtgga
     6361 ggccgaaagt acatgtttcg catgggaacc ccagaccctg agtacccaga tgactacagc
     6421 caaggtggga ccaggctgga cgggaagaat ctggtgcagg aatggctggc gaagcgccag
     6481 ggtgcccggt atgtgtggaa ccgcactgag ctcatgcagg cttccctgga cccgtctgtg
     6541 acccatctca tgggtctctt tgagcctgga gacatgaaat acgagatcca ccgagactcc
     6601 acactggacc cctccctgat ggagatgaca gaggctgccc tgcgcctgct gagcaggaac
     6661 ccccgcggct tcttcctctt cgtggagggt ggtcgcatcg accatggtca tcatgaaagc
     6721 agggcttacc gggcactgac tgagacgatc atgttcgacg acgccattga gagggcgggc
     6781 cagctcacca gcgaggagga cacgctgagc ctcgtcactg ccgaccactc ccacgtcttc
     6841 tccttcggag gctaccccct gcgagggagc tccatcttcg ggctggcccc tggcaaggcc
     6901 cgggacagga aggcctacac ggtcctccta tacggaaacg gtccaggcta tgtgctcaag
     6961 gacggcgccc ggccggatgt taccgagagc gagagcggga gccccgagta tcggcagcag
     7021 tcagcagtgc ccctggacga agagacccac gcaggcgagg acgtggcggt gttcgcgcgc
     7081 ggcccgcagg cgcacctggt tcacggcgtg caggagcaga ccttcatagc gcacgtcatg
     7141 gccttcgccg cctgcctgga gccctacacc gcctgcgacc tggcgccccc cgccggcacc
     7201 accgacgccg cgcacccggg tggcggagga ctgaacgaca tcttcgaggc tcagaaaatc
     7261 gaatggcacg aacatcacca tcaccatcac catcaccatt aaggccggcc tgtagtcact
     7321 ggcacaacgc gttacccggt aagccaatcg ggtatacacg gtcgtcatac tgcagacagg
     7381 gttcttctac tttgcaagat agtcttgagt agtaaaataa atagatagag aaaatcagcc
     7441 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7501 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcacaa
     7561 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7621 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     7681 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7741 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     7801 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7861 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7921 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     7981 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     8041 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     8101 gacaaggtga ggaactaaac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     8161 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     8221 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     8281 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     8341 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     8401 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     8461 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     8521 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     8581 gccggggtgc ccgccttcct ggagacatcc gcgccccgca acctcccctt ctacgagcgg
     8641 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     8701 acccgcaagc ccggtgcctg acacgtgcta cgagatttcg attccaccgc cgccttctat
     8761 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8821 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     8881 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8941 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     9001 tagagcttgg cgtaatcatg gtcattacca atgcttaatc agtgaggcac ctatctcagc
     9061 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9121 acgggagggc ttaccatctg gccccagcgc tgcgatgata ccgcgagaac cacgctcacc
     9181 ggctccggat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9241 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9301 ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct acaggcatcg tggtgtcacg
     9361 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9421 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9481 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9541 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9601 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9661 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9721 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9781 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9841 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatattct tcctt
//
