LOCUS       sequence-304249-        9955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-CD101-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5560..5595
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5605..5628
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7288..7332
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7333..7359
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7499..7516)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7505..7616
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7734..7754)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7739..8068
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7919..8054
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7981..8000
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8116..8163
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8182..8781
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8182..8201)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8678..8698
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8911..9032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8948..8967)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9002..9021
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9085..9876,9877..9945))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9708..9727
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9946..9955,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccca gagagaagtg
     2761 acagttcaga agggccctct gttcagagcc gagggctatc ctgtgtccat cggctgtaat
     2821 gtgacaggcc accagggacc tagcgagcag catttccagt ggtccgtgta cctgcctaca
     2881 aaccccacac aagaggtgca gatcatcagc accaaggacg ccgcctttag ctacgccgtg
     2941 tatactcaga gagtccgctc tggcgacgtg tacgtggaaa gagtgcaggg caatagcgtg
     3001 ctgctgcaca tcagcaagct gcagatgaag gatgccggcg agtacgagtg tcacacccct
     3061 aacaccgacg agaagtacta cggcagctac agcgccaaga ccaacctgat cgtgatcccc
     3121 gatacactga gcgccaccat gtctagccag acactgggaa aagaggaagg cgagcctctg
     3181 gctctgacct gtgaagcctc taaagccacc gctcagcaca cccacctgag cgtgacatgg
     3241 tatctgactc aggatggcgg cggaagccag gccacagaga tcattagcct gagcaaggac
     3301 ttcatcctgg tgcctggacc tctgtacacc gagagatttg ccgcttccga cgtgcagctg
     3361 aacaagctgg gccctaccac cttcagactg agcatcgaaa gactgcagag cagcgaccag
     3421 ggccagctgt tttgtgaagc caccgagtgg attcaggacc ccgacgagac atggatgttc
     3481 atcaccaaga agcagaccga ccagaccaca ctgagaatcc agcctgccgt gaaggacttc
     3541 caagtgaaca tcaccgccga cagcctgttt gccgagggaa aacctctgga actcgtgtgc
     3601 ctggtggtgt cctctggaag agatcctcag ctgcaaggca tctggttctt caacggaacc
     3661 gagatcgccc acatcgatgc tggcggagtg ctgggcctga agaacgacta caaagagaga
     3721 gccagccagg gcgagctgca agtgtctaaa ctgggaccta aggccttcag cctgaagatt
     3781 ttcagtctgg gccctgagga cgagggcgcc tatagatgtg tggtggccga agtgatgaag
     3841 accagaacag gctcttggca ggtcctgcag agaaagcaga gccccgatag ccacgtgcac
     3901 ctgagaaaac ctgccgccag atctgtcgtg atgagcacca agaacaagca gcaggtcgtg
     3961 tgggaaggcg aaaccctggc ctttctgtgt aaagccggcg gagctgagag ccctttgtct
     4021 gtgtcttggt ggcacatccc cagagatcag acccagcctg agtttgtggc cggcatggga
     4081 caagatggca ttgtgcagct gggagccagc tacggcgtgc catcttatca cggcaacacc
     4141 cggctggaaa agatggactg ggccaccttt cagctggaaa tcaccttcac cgccatcacc
     4201 gatagcggca cctacgagtg cagagtgtcc gagaagtcca gaaatcaggc ccgggatctg
     4261 agctggaccc agaaaatcag cgtgaccgtg aagtccctgg aaagcagcct gcaggtttcc
     4321 ctgatgagca gacagcccca agtgatgctg accaacacct tcgacctgag ctgtgtcgtc
     4381 agagccggct attctgacct gaaggtgcca ctgaccgtga cctggcagtt tcagcctgcc
     4441 agcagccaca tcttccacca gctgatcaga atcacccaca acggcacaat cgagtggggc
     4501 aacttcctga gccggttcca gaaaaagacc aaggtgtcac agtccctgtt tagaagccag
     4561 ctgctggtgc acgacgccac agaggaagaa acaggcgtct accagtgcga ggtggaagtg
     4621 tacgaccgga acagcctgta caacaacaga cctcctagag ccagcgccat ctctcaccct
     4681 ctgagaattg ccgtgacact gcccgagagc aagctgaaag tgaacagcag atcccaggtg
     4741 caagagctga gcatcaacag caacaccgac atcgagtgca gcatcctgag caggtccaat
     4801 ggcaacctgc agctggccat catctggtac tttagccccg tgtccaccaa cgcctcttgg
     4861 ctgaagatcc tggaaatgga ccagacaaac gtgatcaaga ccggcgacga gtttcacaca
     4921 ccccagcgga agcagaagtt ccacacagag aaggtgtccc aggacctgtt ccagctgcat
     4981 atcctgaacg tggaagatag cgaccggggc aagtaccact gtgccgtgga agaatggctc
     5041 ctgagcacaa acggcacctg gcacaaactg ggcgagaaga agtctggcct gaccgagctg
     5101 aagctgaagc ctacaggctc taaagtgcgg gtgtccaagg tgtactggac cgagaatgtg
     5161 accgagcata gagaagtggc catccggtgt agcctggaat ctgtgggaag cagcgccaca
     5221 ctgtacagcg tgatgtggta ctggaacaga gagaacagcg gcagcaagct cctggtgcat
     5281 ctgcaacacg atggcctgct ggaatatggc gaggaaggac tgcggcggca tctgcactgt
     5341 tacagaagca gctccaccga cttcgtcctg aagctgcacc aggtcgagat ggaagatgcc
     5401 ggaatgtact ggtgtagagt ggccgagtgg cagctgcatg gacaccctag caaatggatc
     5461 aaccaggcca gcgacgagtc ccagagaatg gtgctgacag tgctgccaag cgagcccaca
     5521 ctgcctagca gaatctgtag ctctgcccct ggcgcgccgg aagatcaagt tgatccaaga
     5581 ttgattgatg gcaagggcgg atctctggag gtgctgttcc agggaccagg cggaagcggc
     5641 aatcccggcg gcgatctggc cccccagatg ctgcgcgagc tgcaggagac caatgccgcc
     5701 ctgcaggacg tgcgcgagct gctgcgccac cgcgtgaagg agatcacctt cctgaagaac
     5761 accgtgatgg agtgcgacgc ctgcggtatg cagccctcta gaggctccgg ctccatcatc
     5821 ccagttgagg aggagaaccc ggacttctgg aaccgcgagg cagccgaggc cctgggtgcc
     5881 gccaagaagc tgcagcctgc acagacagcc gccaagaacc tcatcatctt cctgggcgat
     5941 gggatggggg tgtctacggt gacagctgcc aggatcctaa aagggcagaa gaaggacaaa
     6001 ctggggcctg agatacccct ggccatggac cgcttcccat atgtggctct gtccaagaca
     6061 tacaatgtag acaaacatgt gccagacagt ggagccacag ccacggccta cctgtgcggg
     6121 gtcaagggca acttccagac cattggcttg agtgcagccg cccgctttaa ccagtgcaac
     6181 acgacacgcg gcaacgaggt catctccgtg atgaatcggg ccaagaaagc agggaagtca
     6241 gtgggagtgg taaccaccac acgagtgcag cacgcctcgc cagccggcac ctacgcccac
     6301 acggtgaacc gcaactggta ctcggacgcc gacgtgcctg cctcggcccg ccaggagggg
     6361 tgccaggaca tcgctacgca gctcatctcc aacatggaca ttgacgtgat cctaggtgga
     6421 ggccgaaagt acatgtttcg catgggaacc ccagaccctg agtacccaga tgactacagc
     6481 caaggtggga ccaggctgga cgggaagaat ctggtgcagg aatggctggc gaagcgccag
     6541 ggtgcccggt atgtgtggaa ccgcactgag ctcatgcagg cttccctgga cccgtctgtg
     6601 acccatctca tgggtctctt tgagcctgga gacatgaaat acgagatcca ccgagactcc
     6661 acactggacc cctccctgat ggagatgaca gaggctgccc tgcgcctgct gagcaggaac
     6721 ccccgcggct tcttcctctt cgtggagggt ggtcgcatcg accatggtca tcatgaaagc
     6781 agggcttacc gggcactgac tgagacgatc atgttcgacg acgccattga gagggcgggc
     6841 cagctcacca gcgaggagga cacgctgagc ctcgtcactg ccgaccactc ccacgtcttc
     6901 tccttcggag gctaccccct gcgagggagc tccatcttcg ggctggcccc tggcaaggcc
     6961 cgggacagga aggcctacac ggtcctccta tacggaaacg gtccaggcta tgtgctcaag
     7021 gacggcgccc ggccggatgt taccgagagc gagagcggga gccccgagta tcggcagcag
     7081 tcagcagtgc ccctggacga agagacccac gcaggcgagg acgtggcggt gttcgcgcgc
     7141 ggcccgcagg cgcacctggt tcacggcgtg caggagcaga ccttcatagc gcacgtcatg
     7201 gccttcgccg cctgcctgga gccctacacc gcctgcgacc tggcgccccc cgccggcacc
     7261 accgacgccg cgcacccggg tggcggagga ctgaacgaca tcttcgaggc tcagaaaatc
     7321 gaatggcacg aacatcacca tcaccatcac catcaccatt aaggccggcc tgtagtcact
     7381 ggcacaacgc gttacccggt aagccaatcg ggtatacacg gtcgtcatac tgcagacagg
     7441 gttcttctac tttgcaagat agtcttgagt agtaaaataa atagatagag aaaatcagcc
     7501 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7561 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcacaa
     7621 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7681 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     7741 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7801 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     7861 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7921 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7981 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     8041 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     8101 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     8161 gacaaggtga ggaactaaac catgaccgag tacaagccca cggtgcgcct cgccacccgc
     8221 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg
     8281 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc
     8341 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg
     8401 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg
     8461 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg
     8521 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac
     8581 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc
     8641 gccggggtgc ccgccttcct ggagacatcc gcgccccgca acctcccctt ctacgagcgg
     8701 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg
     8761 acccgcaagc ccggtgcctg acacgtgcta cgagatttcg attccaccgc cgccttctat
     8821 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8881 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     8941 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9001 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     9061 tagagcttgg cgtaatcatg gtcattacca atgcttaatc agtgaggcac ctatctcagc
     9121 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9181 acgggagggc ttaccatctg gccccagcgc tgcgatgata ccgcgagaac cacgctcacc
     9241 ggctccggat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9301 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9361 ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct acaggcatcg tggtgtcacg
     9421 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9481 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9541 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9601 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9661 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9721 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9781 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9841 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9901 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatattct tcctt
//
