LOCUS       sequence-304251-        9967 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-CDON-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5572..5607
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5617..5640
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7300..7344
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7345..7371
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7511..7528)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7517..7628
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7746..7766)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7751..8080
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7931..8066
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7993..8012
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8128..8175
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8194..8793
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8194..8213)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8690..8710
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8923..9044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8960..8979)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9014..9033
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9097..9888,9889..9957))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9720..9739
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9958..9967,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccga tctggcccct
     2761 tactttacat ctgaacctct gagcgccgtg cagaaactcg gaggacctgt ggttctgcac
     2821 tgtagcgctc agcctgtgac caccagaatc agctggctgc acaacggcaa gaccctggac
     2881 ggaaacctgg aacacgtgaa gatccaccag ggcaccctga ccatcctgag cctgaatagc
     2941 agcctgctgg gctactacca gtgcctggcc aacaattcta tcggcgccat tgtgtctggc
     3001 cctgccacag tgtctgtggc cgttctgggc gattttggca gcagcaccaa acacgtgatc
     3061 accgccgagg aaaagagcgc cggctttatc ggatgtagag tgcccgagag caaccccaag
     3121 gccgaagtgc ggtataagat cagaggcaag tggctggaac acagcaccga gaactacctg
     3181 atcctgccaa gcggcaacct gcagatcctg aacgtgtccc tggaagataa gggcagctac
     3241 aagtgcgccg cctacaatcc agtgacacac cagctgaagg tggaacccat cggcagaaag
     3301 ctgctggtgt ccagacctag cagcgacgac gtgcacattc tgcaccctac acattctcag
     3361 gccctggccg tgctgagcag atctcctgtt acactggaat gcgtggtgtc cggcgttcca
     3421 gctcctcagg tgtactggct gaaggacgga caggatatcg cccctggcag caattggaga
     3481 aggctgtact ctcacctggc caccgattct gtggaccctg ccgatagcgg caattactcc
     3541 tgtatggccg gcaacaagag cggcgacgtg aaatacgtga cctacatggt caacgtgctg
     3601 gaacatgcca gcatcagcaa gggcctgcag gaccagattg tgtctctggg cgccaccgtg
     3661 cacttcacct gtgatgtgca tggcaacccc gctcctaact gcacctggtt tcacaacgcc
     3721 cagcctattc accctagcgc cagacatctg acagccggca atggactgaa gatcagcggc
     3781 gtgacagtgg aagatgtggg catgtaccag tgtgtggccg acaacggcat cggctttatg
     3841 cactctaccg gcagactgga aatcgagaac gacggcggct tcaagcccgt gatcattaca
     3901 gcccctgtgt ccgccaaagt ggccgatggg gattttgtga ccctgagctg taatgcctct
     3961 ggcctgcctg tgcctgtgat ccgttggtac gatagccacg gcctgatcac cagccatcct
     4021 agccaggtgc tgcggagcaa gagcagaaag tctcagctgt caagacccga gggcctgaat
     4081 ctggaacccg tgtactttgt tctgagccag gctggcgcct ctagcctgca cattcaggct
     4141 gtgacacaag agcacgccgg caagtatatc tgcgaggccg ccaatgagca cggcacaact
     4201 caagccgaag ccagcctgat ggtggtgccc ttcgagacaa acacaaaggc cgagacagtg
     4261 accctgcctg acgccgctca gaacgacgac agatctaaga gagatggcag cgagacaggc
     4321 ctgctgtcca gctttcctgt gaaagtgcac cccagcgccg tggaatctgc ccctgagaaa
     4381 aatgccagcg gcatcagcgt gcccgacgct cctattattc tgagccctcc tcagacacac
     4441 acccctgaca cctacaacct cgtttggaga gccggaaagg atggcggcct gcctatcaac
     4501 gcctacttcg tgaagtaccg gaagctggac gatggcgtgg gaatgctcgg atcttggcac
     4561 acagtcagag tgccaggctc cgagaatgag ctgcatctgg ccgaactgga accttccagc
     4621 ctgtacgagg tgctgatggt tgctagatct gctgccggcg aaggacagcc tgccatgctg
     4681 accttccgga ccagcaaaga gaaaaccgcc agcagcaaga acacccaggc ctcttctcca
     4741 cctgtgggca tccctaagta ccccgtggtg tctgaagccg ctaacaacaa ctttggcgtg
     4801 gtgctgaccg acagcagcag acatagcgga gtgcctgagg ctcccgatag acctacaatc
     4861 agcaccgcca gcgaaaccag cgtgtacgtg acatggattc ccagagccaa tggcggcagc
     4921 cctatcaccg ccttcaaggt cgagtacaag cggatgagaa ccagcaactg gctggtggcc
     4981 gccgaagata ttcctcctag caagctgagc gtggaagtgc gctctctgga accaggcagc
     5041 acctacaagt tcagagtgat cgccatcaac cactacggcg agagcttcag atccagcgcc
     5101 agcagacctt accaggtcgt gggcttcccc aacagattca gcagcaggcc aatcacaggc
     5161 cctcacattg cctacaccga ggccgtgtct gacacccaga tcatgctgaa gtggacctac
     5221 attcccagct ccaacaacaa cacccctatc cagggcttct acatctacta cagacccacc
     5281 gacagcgaca acgactccga ctacaagcga gatgtggtgg aaggcagcaa gcagtggcac
     5341 atgattggcc atctgcagcc cgagacaagc tacgacatca agatgcagtg cttcaacgaa
     5401 ggcggcgagt ccgagttcag caacgtgatg atctgcgaga caaaagtgaa gagagtgcct
     5461 ggggccagcg agtaccctgt gaaggatctg agcacccctc ctaattctct cggctctggc
     5521 ggaaatgtgg gcccagctac atctcctgcc agatcctctg atggcgcgcc ggaagatcaa
     5581 gttgatccaa gattgattga tggcaagggc ggatctctgg aggtgctgtt ccagggacca
     5641 ggcggaagcg gcaatcccgg cggcgatctg gccccccaga tgctgcgcga gctgcaggag
     5701 accaatgccg ccctgcagga cgtgcgcgag ctgctgcgcc accgcgtgaa ggagatcacc
     5761 ttcctgaaga acaccgtgat ggagtgcgac gcctgcggta tgcagccctc tagaggctcc
     5821 ggctccatca tcccagttga ggaggagaac ccggacttct ggaaccgcga ggcagccgag
     5881 gccctgggtg ccgccaagaa gctgcagcct gcacagacag ccgccaagaa cctcatcatc
     5941 ttcctgggcg atgggatggg ggtgtctacg gtgacagctg ccaggatcct aaaagggcag
     6001 aagaaggaca aactggggcc tgagataccc ctggccatgg accgcttccc atatgtggct
     6061 ctgtccaaga catacaatgt agacaaacat gtgccagaca gtggagccac agccacggcc
     6121 tacctgtgcg gggtcaaggg caacttccag accattggct tgagtgcagc cgcccgcttt
     6181 aaccagtgca acacgacacg cggcaacgag gtcatctccg tgatgaatcg ggccaagaaa
     6241 gcagggaagt cagtgggagt ggtaaccacc acacgagtgc agcacgcctc gccagccggc
     6301 acctacgccc acacggtgaa ccgcaactgg tactcggacg ccgacgtgcc tgcctcggcc
     6361 cgccaggagg ggtgccagga catcgctacg cagctcatct ccaacatgga cattgacgtg
     6421 atcctaggtg gaggccgaaa gtacatgttt cgcatgggaa ccccagaccc tgagtaccca
     6481 gatgactaca gccaaggtgg gaccaggctg gacgggaaga atctggtgca ggaatggctg
     6541 gcgaagcgcc agggtgcccg gtatgtgtgg aaccgcactg agctcatgca ggcttccctg
     6601 gacccgtctg tgacccatct catgggtctc tttgagcctg gagacatgaa atacgagatc
     6661 caccgagact ccacactgga cccctccctg atggagatga cagaggctgc cctgcgcctg
     6721 ctgagcagga acccccgcgg cttcttcctc ttcgtggagg gtggtcgcat cgaccatggt
     6781 catcatgaaa gcagggctta ccgggcactg actgagacga tcatgttcga cgacgccatt
     6841 gagagggcgg gccagctcac cagcgaggag gacacgctga gcctcgtcac tgccgaccac
     6901 tcccacgtct tctccttcgg aggctacccc ctgcgaggga gctccatctt cgggctggcc
     6961 cctggcaagg cccgggacag gaaggcctac acggtcctcc tatacggaaa cggtccaggc
     7021 tatgtgctca aggacggcgc ccggccggat gttaccgaga gcgagagcgg gagccccgag
     7081 tatcggcagc agtcagcagt gcccctggac gaagagaccc acgcaggcga ggacgtggcg
     7141 gtgttcgcgc gcggcccgca ggcgcacctg gttcacggcg tgcaggagca gaccttcata
     7201 gcgcacgtca tggccttcgc cgcctgcctg gagccctaca ccgcctgcga cctggcgccc
     7261 cccgccggca ccaccgacgc cgcgcacccg ggtggcggag gactgaacga catcttcgag
     7321 gctcagaaaa tcgaatggca cgaacatcac catcaccatc accatcacca ttaaggccgg
     7381 cctgtagtca ctggcacaac gcgttacccg gtaagccaat cgggtataca cggtcgtcat
     7441 actgcagaca gggttcttct actttgcaag atagtcttga gtagtaaaat aaatagatag
     7501 agaaaatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7561 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7621 attgcatcac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7681 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     7741 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     7801 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     7861 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     7921 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7981 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     8041 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     8101 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     8161 atagtataat acgacaaggt gaggaactaa accatgaccg agtacaagcc cacggtgcgc
     8221 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     8281 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     8341 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     8401 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     8461 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     8521 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     8581 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     8641 gcggccgagc gcgccggggt gcccgccttc ctggagacat ccgcgccccg caacctcccc
     8701 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     8761 acctggtgca tgacccgcaa gcccggtgcc tgacacgtgc tacgagattt cgattccacc
     8821 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     8881 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     8941 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9001 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     9061 tcgacctcta gctagagctt ggcgtaatca tggtcattac caatgcttaa tcagtgaggc
     9121 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9181 gataactacg atacgggagg gcttaccatc tggccccagc gctgcgatga taccgcgaga
     9241 accacgctca ccggctccgg atttatcagc aataaaccag ccagccggaa gggccgagcg
     9301 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9361 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccatcg ctacaggcat
     9421 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9481 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9541 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9601 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9661 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9721 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9781 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9841 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     9901 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatatt
     9961 cttcctt
//
