LOCUS       sequence-304257-       10018 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PTPRQ(Trunc3)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5623..5658
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5668..5691
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7351..7395
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7396..7422
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7562..7579)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7568..7679
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7797..7817)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7802..8131
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7982..8117
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8044..8063
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8179..8226
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8245..8844
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8245..8264)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8741..8761
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8974..9095
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9011..9030)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9065..9084
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9148..9939,9940..10008))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9771..9790
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10009..10018,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgcccc tccaaaggat
     2761 gtgtactacg ccaatctgag cagcagctcc atcatcctgt tctggacccc tccaagcaag
     2821 cccaacggca tcatccagta ctacagcgtg tactaccgga acaccagcgg caccttcatg
     2881 cagaacttca ccctgcacga agtgaccaac gacttcgaca acatgaccgt gtccaccatc
     2941 atcgacaagc tgaccatctt cagctactac accttctggc tgaccgccag cacaagcgtc
     3001 ggcaatggca acaagagcag cgacatcatc gaggtgtaca cagaccagga catccctgag
     3061 ggcttcgtgg gcaatctgac ctacgagagc atcagcagca ccgccatcaa tgtgtcttgg
     3121 gtgccaccag ctcagcccaa tggcctggtg ttttactacg tgtccctgat cctgcagcag
     3181 acccctagac atgttcggcc tccactggtc acatacgagc ggagcatcta cttcgataac
     3241 ctcgagaagt acaccgacta catcctgaag atcaccccta gcaccgagaa gggcttcagc
     3301 gatacctaca cagcccagct gtacatcaag accgaagagg acgtgcccga gacaagcccc
     3361 atcatcaaca ccttcaagaa cctgtccagc accagcgtgc tgctgtcttg ggaccctcct
     3421 gtgaagccta acggcgccat catctcctac gatctgacac tgcagggccc caacgagaac
     3481 tacagcttca tcaccagcga caactacatc attctggaag aactgagccc cttcacgctc
     3541 tacagctttt ttgccgccgc tcggaccaga aaaggcctgg gacctagcag catcctgttt
     3601 ttctacaccg acgagagcgt gcccctggct cctcctcaga atctgaccct gatcaactgc
     3661 acctccgact tcgtgtggct gaagtggtcc ccttctcctc tgcctggcgg aatcgtgaag
     3721 gtgtacagct ttaagatcca cgagcacgag acagacacca tctactacaa gaacatcagc
     3781 ggctttaaga ccgaggccaa gctcgtggga ctcgagcctg tgtctaccta cagcatcaga
     3841 gtgtccgcct tcaccaaagt tggcaacggc aaccagttca gcaacgtggt caagttcacc
     3901 acacaagagt ccgtgcctga cgtggtgcag aacatgcagt gtatggccac aagctggcag
     3961 agcgtgctgg ttaagtggga cccacctaag aaggccaacg gaatcattac ccagtacatg
     4021 gtcaccgtgg aacggaacag caccaaggtg tccccacagg accacatgta cacattcatc
     4081 aagctgctgg ccaacaccag ctacgtgttc aaagtgcggg cctctacatc tgccggcgag
     4141 ggcgacgaaa gcacatgtca tgtgtccaca ctgcctgaga cagtgcccag cgtgcccacc
     4201 aatatcgcct tttccgatgt gcagagcacc tccgccacac tgacctggat cagacctgat
     4261 accatcctgg gctacttcca gaactacaag atcacaaccc agctgcgggc ccagaaatgc
     4321 aaagagtggg agagcgagga atgcgtcgag taccagaaga tccagtacct gtacgaggcc
     4381 cacctgaccg aggaaaccgt gtacggcctg aagaaattcc ggtggtacag attccaggtg
     4441 gccgcctcta caaacgccgg ctacggaaat gccagcaact ggatcagcac aaagaccctg
     4501 cctggacctc ctgacggccc tcctgaaaat gtgcatgtgg tggccacatc tcccttcagc
     4561 atctccatct cttggagcga gcctgccgtg atcacaggcc ctacctgtta cctgatcgac
     4621 gtgaagtccg tggacaacga cgagttcaac atctccttca tcaagagcaa cgaggaaaac
     4681 aagaccatcg agatcaagga cctggaaatc ttcacccggt acagcgtggt catcaccgcc
     4741 tttacaggca atatcagcgc cgcctacgtg gaaggcaagt cctctgccga gatgatcgtg
     4801 accacactgg aaagcgcccc taaggaccct ccaaacaata tgaccttcca gaaaatcccc
     4861 gacgaagtca ccaagttcca gctgaccttc ctgcctccta gccagcctaa tggcaatatc
     4921 caggtgtacc aggctctggt gtacagagag gatgatccca cagccgtgca gatccacaac
     4981 ctgtctatca tccagaaaac gaacaccttc gtgatcgcca tgctggaagg actgaaaggc
     5041 gggcacacct acaatatctc cgtgtacgcc gtgaatagcg ctggcgcagg acctaaggtg
     5101 cccatgagaa tcaccatgga catcaaggcc cctgccagac ctaagaccaa gcctacacct
     5161 atctacgacg ccaccggcaa gctgctcgtg accagcacca caatcaccat cagaatgccc
     5221 atctgctact actccgacga tcacggcccc atcaagaacg tgcaggtcct ggttacagaa
     5281 acaggcgccc agcacgacgg caacgtgaca aagtggtacg acgcctactt caacaaggcc
     5341 agaccttact tcaccaacga gggcttcccc aatcctcctt gtaccgaggg caagaccaag
     5401 ttcagcggca acgaagagat ctatatcatc ggagccgaca acgcctgcat gatccccggc
     5461 aatgaggaca agatctgcaa tggccctctg aagcccaaga agcagtatct gttcaagttc
     5521 cgggccacca acatcatggg ccagttcacc gacagcgact acagcgaccc tgtgaaaaca
     5581 ctcggcgagg gactgagcga gagaaccgtt gaaggcgcgc cggaagatca agttgatcca
     5641 agattgattg atggcaaggg cggatctctg gaggtgctgt tccagggacc aggcggaagc
     5701 ggcaatcccg gcggcgatct ggccccccag atgctgcgcg agctgcagga gaccaatgcc
     5761 gccctgcagg acgtgcgcga gctgctgcgc caccgcgtga aggagatcac cttcctgaag
     5821 aacaccgtga tggagtgcga cgcctgcggt atgcagccct ctagaggctc cggctccatc
     5881 atcccagttg aggaggagaa cccggacttc tggaaccgcg aggcagccga ggccctgggt
     5941 gccgccaaga agctgcagcc tgcacagaca gccgccaaga acctcatcat cttcctgggc
     6001 gatgggatgg gggtgtctac ggtgacagct gccaggatcc taaaagggca gaagaaggac
     6061 aaactggggc ctgagatacc cctggccatg gaccgcttcc catatgtggc tctgtccaag
     6121 acatacaatg tagacaaaca tgtgccagac agtggagcca cagccacggc ctacctgtgc
     6181 ggggtcaagg gcaacttcca gaccattggc ttgagtgcag ccgcccgctt taaccagtgc
     6241 aacacgacac gcggcaacga ggtcatctcc gtgatgaatc gggccaagaa agcagggaag
     6301 tcagtgggag tggtaaccac cacacgagtg cagcacgcct cgccagccgg cacctacgcc
     6361 cacacggtga accgcaactg gtactcggac gccgacgtgc ctgcctcggc ccgccaggag
     6421 gggtgccagg acatcgctac gcagctcatc tccaacatgg acattgacgt gatcctaggt
     6481 ggaggccgaa agtacatgtt tcgcatggga accccagacc ctgagtaccc agatgactac
     6541 agccaaggtg ggaccaggct ggacgggaag aatctggtgc aggaatggct ggcgaagcgc
     6601 cagggtgccc ggtatgtgtg gaaccgcact gagctcatgc aggcttccct ggacccgtct
     6661 gtgacccatc tcatgggtct ctttgagcct ggagacatga aatacgagat ccaccgagac
     6721 tccacactgg acccctccct gatggagatg acagaggctg ccctgcgcct gctgagcagg
     6781 aacccccgcg gcttcttcct cttcgtggag ggtggtcgca tcgaccatgg tcatcatgaa
     6841 agcagggctt accgggcact gactgagacg atcatgttcg acgacgccat tgagagggcg
     6901 ggccagctca ccagcgagga ggacacgctg agcctcgtca ctgccgacca ctcccacgtc
     6961 ttctccttcg gaggctaccc cctgcgaggg agctccatct tcgggctggc ccctggcaag
     7021 gcccgggaca ggaaggccta cacggtcctc ctatacggaa acggtccagg ctatgtgctc
     7081 aaggacggcg cccggccgga tgttaccgag agcgagagcg ggagccccga gtatcggcag
     7141 cagtcagcag tgcccctgga cgaagagacc cacgcaggcg aggacgtggc ggtgttcgcg
     7201 cgcggcccgc aggcgcacct ggttcacggc gtgcaggagc agaccttcat agcgcacgtc
     7261 atggccttcg ccgcctgcct ggagccctac accgcctgcg acctggcgcc ccccgccggc
     7321 accaccgacg ccgcgcaccc gggtggcgga ggactgaacg acatcttcga ggctcagaaa
     7381 atcgaatggc acgaacatca ccatcaccat caccatcacc attaaggccg gcctgtagtc
     7441 actggcacaa cgcgttaccc ggtaagccaa tcgggtatac acggtcgtca tactgcagac
     7501 agggttcttc tactttgcaa gatagtcttg agtagtaaaa taaatagata gagaaaatca
     7561 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     7621 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatca
     7681 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     7741 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     7801 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7861 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     7921 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7981 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     8041 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     8101 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     8161 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     8221 tacgacaagg tgaggaacta aaccatgacc gagtacaagc ccacggtgcg cctcgccacc
     8281 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     8341 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     8401 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     8461 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     8521 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     8581 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     8641 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     8701 cgcgccgggg tgcccgcctt cctggagaca tccgcgcccc gcaacctccc cttctacgag
     8761 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     8821 atgacccgca agcccggtgc ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     8881 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     8941 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     9001 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     9061 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     9121 agctagagct tggcgtaatc atggtcatta ccaatgctta atcagtgagg cacctatctc
     9181 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9241 gatacgggag ggcttaccat ctggccccag cgctgcgatg ataccgcgag aaccacgctc
     9301 accggctccg gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9361 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9421 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatc gctacaggca tcgtggtgtc
     9481 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9541 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9601 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9661 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9721 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9781 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9841 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9901 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9961 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatat tcttcctt
//
