LOCUS       sequence-304259-       10024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PTPRQ(Trunc2)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5629..5664
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5674..5697
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7357..7401
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7402..7428
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7568..7585)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7574..7685
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7803..7823)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7808..8137
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7988..8123
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8050..8069
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8185..8232
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8251..8850
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8251..8270)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8747..8767
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8980..9101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9017..9036)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9071..9090
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9154..9945,9946..10014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9777..9796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10015..10024,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccga aagataccaa
     2761 gtggacctgg tgcctgacag cggcttcgtg accatcagag atctcggcgg aggcgagtat
     2821 caggtggacg tgtcaaatgt ggtgcccggc acaagatacg acatcaccat cagcagcatc
     2881 agcaccacct acacaagccc cgtgaccaga atcgtgacca ccaacgtgac caagcctgga
     2941 cctcctgtgt ttctggccgg cgagagagtt ggatctgccg gcattctgct gagctggaac
     3001 acccctccta atcctaacgg ccggatcatc agctacatcg tgaagtacaa agaagtgtgc
     3061 ccctggatgc agaccgtgta cacacaagtg cggagcaagc ccgacagcct ggaagtgctg
     3121 ctgaccaacc tgaatcctgg cacaacctac gagatcaagg tggccgccga gaattctgcc
     3181 ggaatcggcg tgttcagcga ccccttcctg tttcaaaccg ccgaatctgc ccctggcaag
     3241 gtggtcaatc tgaccgtgga agcctacaac gccagcgccg tgaagctgat ctggtatctg
     3301 cccagacagc ccaacggcaa gatcaccagc ttcaagatca gcgtgaagca cgctcggagc
     3361 ggcatcgtgg tcaaggacgt gtccattaga gtggaagata tcctgaccgg caagctgccc
     3421 gagtgcaacg agaatagcga gagcttcctg tggtccaccg cctctccatc tccaacactg
     3481 ggcagagtga cccctccaag cagaaccaca cacagcagct ccacactgac ccagaacgag
     3541 atcagcagcg tgtggaaaga acccatcagc ttcgtggtca cccacctgag gccttacacc
     3601 acatacctgt tcgaggtgtc cgccgtgaca acagaggccg gctacatcga tagcaccatc
     3661 gtgcggacac cagagagcgt gccagaaggc cctcctcaga attgcgtgac aggcaacatc
     3721 accggcaaga gcttcagcat cctgtgggac cctccaacca tcgtgaccgg aaagttcagc
     3781 tacagagtgg aactgtacgg ccccagcggc agaatcctgg ataacagcac caaggacctg
     3841 aagttcgcct tcaccaatct gacacccttc actatgtacg atgtgtatat cgccgctgag
     3901 acaagcgccg gcacaggccc taagagcaac atcagcgtgt tcacccctcc agatgtgcct
     3961 ggcgccgtgt ttgatctgca gctggccgag gtggaatcta cccaagtccg gatcacctgg
     4021 aagaagccca ggcaacctaa cggcatcatc aaccagtatc gcgtgaaggt gctggtgccc
     4081 gagacaggca tcatcctgga aaacaccctg ctgaccggga acaacgagta catcaacgac
     4141 cccatggctc ccgagatcgt gaacatcgtg gaacccatgg tcggactgta cgagggctct
     4201 gccgagatgt ctagcgatct gcacagcctg gccacattca tctacaactc tcaccccgac
     4261 aagaacttcc ccgccagaaa tagagccgag gaccagacaa gccctgtggt caccaccaga
     4321 aaccagtaca tcaccgatat tgccgccgag cagctgagct acgtgatcag acgactggtg
     4381 cccttcaccg agcacatgat ctccgtgtcc gcctttacca tcatgggcga gggacctcca
     4441 acagtgctgt ctgtgcggac aagacaacag gtgcccagct ccatcaagat catcaattac
     4501 aagaacatca gctcctccag catcctcctg tactgggacc caccagagta ccctaatgga
     4561 aagatcaccc actacaccat ctacgccatg gaactggaca ccaaccgggc cttccagatc
     4621 acaacaatcg acaacagctt cctgatcacc ggcctgaaga agtacaccaa gtacaagatg
     4681 agagtggccg ccagcacaca cgtgggagag tctagcctga gcgaagagaa cgacatcttt
     4741 gtgcggacca gcgaggacga gcctgaaagc tctcctcagg acgtggaagt gatcgacgtg
     4801 accgccgatg agatccggct gaaatggtca cctcctgaga agcccaatgg catcattatc
     4861 gcctacgagg tgctgtacaa gaatatcgac accctgtaca tgaagaacac ctccaccacc
     4921 gatatcatcc tgcggaacct gcggcctcac acactgtaca atatcagcgt gcggagctac
     4981 accagatttg gccacggcaa tcaggtgtcc agcctgctga gcgtcagaac ctctgagaca
     5041 gtgcctgata gcgcccctga gaacatcacc tataagaaca tctccagcgg cgagatcgag
     5101 ctgtccttcc tgccaccttc tagccccaac ggaatcatta agaagtatac catctacctg
     5161 aagcggagca acggcaacga ggaacggacc atcaacacca ccagcctgac acagaacatc
     5221 aaggtgctca aaaagtacac gcagtatatc atcgaagtgt ccgcaagcac cctgaaaggc
     5281 gagggcgtta gaagcgcccc tatcagcatt ctgaccgaag aggacgcccc tgacagccca
     5341 cctcaggatt tttccgtgaa gcagctgtcc ggcgtgaccg tgaaactgtc ttggcagcct
     5401 cctctggaac ccaacgggat tatcctgtac tacaccgtgt acgtgtggaa ccgcagcagc
     5461 ctgaaaacca tcaatgtgac cgagacatcc ctggaactga gcgacctgga ctacaacgtg
     5521 gaatactccg cctacgtgac agccagcaca agattcggag atggcaagac ccggtccaac
     5581 atcattagct ttcagacccc tgagggcgct ccctccgatg gcgcgccgga agatcaagtt
     5641 gatccaagat tgattgatgg caagggcgga tctctggagg tgctgttcca gggaccaggc
     5701 ggaagcggca atcccggcgg cgatctggcc ccccagatgc tgcgcgagct gcaggagacc
     5761 aatgccgccc tgcaggacgt gcgcgagctg ctgcgccacc gcgtgaagga gatcaccttc
     5821 ctgaagaaca ccgtgatgga gtgcgacgcc tgcggtatgc agccctctag aggctccggc
     5881 tccatcatcc cagttgagga ggagaacccg gacttctgga accgcgaggc agccgaggcc
     5941 ctgggtgccg ccaagaagct gcagcctgca cagacagccg ccaagaacct catcatcttc
     6001 ctgggcgatg ggatgggggt gtctacggtg acagctgcca ggatcctaaa agggcagaag
     6061 aaggacaaac tggggcctga gatacccctg gccatggacc gcttcccata tgtggctctg
     6121 tccaagacat acaatgtaga caaacatgtg ccagacagtg gagccacagc cacggcctac
     6181 ctgtgcgggg tcaagggcaa cttccagacc attggcttga gtgcagccgc ccgctttaac
     6241 cagtgcaaca cgacacgcgg caacgaggtc atctccgtga tgaatcgggc caagaaagca
     6301 gggaagtcag tgggagtggt aaccaccaca cgagtgcagc acgcctcgcc agccggcacc
     6361 tacgcccaca cggtgaaccg caactggtac tcggacgccg acgtgcctgc ctcggcccgc
     6421 caggaggggt gccaggacat cgctacgcag ctcatctcca acatggacat tgacgtgatc
     6481 ctaggtggag gccgaaagta catgtttcgc atgggaaccc cagaccctga gtacccagat
     6541 gactacagcc aaggtgggac caggctggac gggaagaatc tggtgcagga atggctggcg
     6601 aagcgccagg gtgcccggta tgtgtggaac cgcactgagc tcatgcaggc ttccctggac
     6661 ccgtctgtga cccatctcat gggtctcttt gagcctggag acatgaaata cgagatccac
     6721 cgagactcca cactggaccc ctccctgatg gagatgacag aggctgccct gcgcctgctg
     6781 agcaggaacc cccgcggctt cttcctcttc gtggagggtg gtcgcatcga ccatggtcat
     6841 catgaaagca gggcttaccg ggcactgact gagacgatca tgttcgacga cgccattgag
     6901 agggcgggcc agctcaccag cgaggaggac acgctgagcc tcgtcactgc cgaccactcc
     6961 cacgtcttct ccttcggagg ctaccccctg cgagggagct ccatcttcgg gctggcccct
     7021 ggcaaggccc gggacaggaa ggcctacacg gtcctcctat acggaaacgg tccaggctat
     7081 gtgctcaagg acggcgcccg gccggatgtt accgagagcg agagcgggag ccccgagtat
     7141 cggcagcagt cagcagtgcc cctggacgaa gagacccacg caggcgagga cgtggcggtg
     7201 ttcgcgcgcg gcccgcaggc gcacctggtt cacggcgtgc aggagcagac cttcatagcg
     7261 cacgtcatgg ccttcgccgc ctgcctggag ccctacaccg cctgcgacct ggcgcccccc
     7321 gccggcacca ccgacgccgc gcacccgggt ggcggaggac tgaacgacat cttcgaggct
     7381 cagaaaatcg aatggcacga acatcaccat caccatcacc atcaccatta aggccggcct
     7441 gtagtcactg gcacaacgcg ttacccggta agccaatcgg gtatacacgg tcgtcatact
     7501 gcagacaggg ttcttctact ttgcaagata gtcttgagta gtaaaataaa tagatagaga
     7561 aaatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     7621 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     7681 gcatcacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7741 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     7801 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7861 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     7921 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     7981 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8041 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     8101 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     8161 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     8221 gtataatacg acaaggtgag gaactaaacc atgaccgagt acaagcccac ggtgcgcctc
     8281 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     8341 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     8401 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     8461 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     8521 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     8581 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     8641 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     8701 gccgagcgcg ccggggtgcc cgccttcctg gagacatccg cgccccgcaa cctccccttc
     8761 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     8821 tggtgcatga cccgcaagcc cggtgcctga cacgtgctac gagatttcga ttccaccgcc
     8881 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     8941 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     9001 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     9061 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     9121 acctctagct agagcttggc gtaatcatgg tcattaccaa tgcttaatca gtgaggcacc
     9181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     9241 aactacgata cgggagggct taccatctgg ccccagcgct gcgatgatac cgcgagaacc
     9301 acgctcaccg gctccggatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     9361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     9421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccatcgcta caggcatcgt
     9481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     9541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     9601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     9661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     9721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     9781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     9841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     9901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     9961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatattctt
    10021 cctt
//
