LOCUS       sequence-304263-       10081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-IGSF10(Trunc3)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5686..5721
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5731..5754
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7414..7458
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7459..7485
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7625..7642)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7631..7742
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7860..7880)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7865..8194
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8045..8180
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8107..8126
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8242..8289
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8308..8907
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8308..8327)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8804..8824
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9037..9158
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9074..9093)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9128..9147
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9211..10002,10003..10071))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9834..9853
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10072..10081,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgcccc tagaatcgtt
     2761 ggaggaaagg ccgccagctt cacaatccct gccaatagcg acgcctttct gccttgcgaa
     2821 gccgtgggaa atcctctgcc taccatccac tggacaagag tgcctagcgg cctggacctg
     2881 agcaagagaa agcagaacag cagagtccag gtgctgccca atggcaccct gagcatccag
     2941 agagtggaaa tccaggacag aggccagtac ctgtgcagcg ccagcaatct gtttggcacc
     3001 gatcatctgc acgtgaccct gagcgtggtg tcttaccctc ctagaatcct ggaacggcgg
     3061 accaaagaaa tcaccgtgca cagcggctcc accgtggaac tgaaatgtag agccgagggc
     3121 agaccctctc ctaccgtgac atggattctg gccaatcaga ccgtggtgtc cgagtctagt
     3181 cagggcagta gacaggccgt ggttaccgtg gatggaaccc tggtgctgca caacctgtcc
     3241 atctacgacc ggggcttcta taagtgcgtg gcctctaatc ctggcggaca ggatagcctg
     3301 ctggtcaaga tccaagtgat cgccgctcct cctgtgattc tggaacagcg gagacaagtg
     3361 attgtcggca catggggcga gagcctgaaa ctgccttgta cagccaaggg cacaccccag
     3421 ccatctgtgt actgggtgct gtctgatggc accgaagtga agcccctgca gtttaccaac
     3481 agcaagctgt tcctgttcag caacggcacc ctgtacatca gaaacctggc cagcagcgac
     3541 agaggcacct acgagtgtat cgccacaagc agcacaggca gcgagagaag agtggtcatg
     3601 ctgaccatgg aagagagagt gacaagcccc agaatcgagg ccgcctctca gaaacggaca
     3661 gaagtgaact tcggcgacaa gctgctgctg aactgtagcg ccacaggcga gcctaagcct
     3721 cagattatgt ggcggctgcc aagcaaagcc gtggtggatc agcagcatag agtcggcagc
     3781 tggattcacg tgtaccctaa tggcagcctg ttcatcggca gcgtgacaga gaaggatagc
     3841 ggcgtgtacc tgtgtgtggc cagaaacaag atgggcgacg acctgatcct gatgcatgtg
     3901 tccctgagac tgaagcccgc caagatcgat cacaagcagt acttcagaaa gcaggtcctg
     3961 cacggcaagg acttccaggt ggactgtaaa gcctctggca gccccgtgcc tgagatttct
     4021 tggagcctgc cagacggcac catgatcaac aatgccatgc aggccgacga cagcggccac
     4081 agaacaagaa gatacaccct gtttaacaac gggaccctct acttcaacaa agtgggcgtc
     4141 gccgaggaag gcgactacac ctgttatgcc cagaacaccc tgggcaaaga cgagatgaag
     4201 gtccacctga ccgtgatcac cgccgctcca aggatcagac agagcaacaa gaccaacaag
     4261 cggatcaagg ccggcgatac agccgtgctg gattgcgaag tgacaggcga ccccaagcca
     4321 aagattttct ggctgctgcc cagcaacgac atgatcagct tcagcatcga ccggtacacc
     4381 ttccacgcca acggcagcct gaccattaac aaagtgaagc tgctggactc cggcgagtac
     4441 gtgtgcgtcg caagaaatcc tagcggcgac gacaccaaga tgtacaagct ggatgtggtg
     4501 tccaagcctc ctctgatcaa cggcctgtac accaaccgga ccgtgattaa ggccacagca
     4561 gtgcggcaca gcaagaagca ctttgactgc agagccgaag ggaccccatc tcctgaagtg
     4621 atgtggatta tgcccgacaa catcttcctg acagcccctt actacggcag ccggatcaca
     4681 gtgcacaaga acggcacact ggaaatccgg aacgtgcggc tgagcgacag cgccgatttc
     4741 atttgtgtgg cccggaatga aggcggcgag tctgtgctgg ttgttcagct ggaagtgctg
     4801 gaaatgctgc ggaggcccac cttcagaaac cccttcaatg agaagatcgt ggctcagctg
     4861 ggcaagagca cagccctgaa ttgctctgtg gacggcaacc ctccacctga gatcatctgg
     4921 attctgccta acggcaccag gttcagcaat ggccctcaga gctaccagta tctgatcgcc
     4981 tccaacggct ccttcatcat cagcaagacc accagagagg acgccggcaa gtatagatgc
     5041 gccgctagaa acaaagtcgg ctacatcgag aagctggtca tcctggaaat cgggcagaaa
     5101 cccgtgatcc tgacatacgc ccctggcaca gtgaagggca tttctggcga aagcctgagc
     5161 ctgcactgtg tgtccgatgg catccccaag cctaacatca agtggaccat gccaagcggc
     5221 tacgtggtgg acagacccca gatcaatggc aagtacatcc tgcacgacaa cggcactctg
     5281 gtcatcaaag aggccaccgc ctacgacagg ggcaactata tctgcaaggc ccagaactcc
     5341 gtgggacaca ccctgattac cgtgcctgtg atgatcgtgg cctatcctcc tcggatcacc
     5401 aacagacctc cacggtctat cgtgaccaga accggcgctg cctttcagct gcattgtgtg
     5461 gctctgggag tgcctaagcc agagatcacc tgggagatgc ctgatcacag cctgctgagc
     5521 accgccagca aagagagaac acacggctct gagcagctgc atctgcaggg caccctggtt
     5581 atccagaatc ctcagaccag cgactccggc atctacaaat gcaccgccaa gaatcctctg
     5641 ggcagcgatt acgccgccac ctacattcaa gtgattggcg cgccggaaga tcaagttgat
     5701 ccaagattga ttgatggcaa gggcggatct ctggaggtgc tgttccaggg accaggcgga
     5761 agcggcaatc ccggcggcga tctggccccc cagatgctgc gcgagctgca ggagaccaat
     5821 gccgccctgc aggacgtgcg cgagctgctg cgccaccgcg tgaaggagat caccttcctg
     5881 aagaacaccg tgatggagtg cgacgcctgc ggtatgcagc cctctagagg ctccggctcc
     5941 atcatcccag ttgaggagga gaacccggac ttctggaacc gcgaggcagc cgaggccctg
     6001 ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat catcttcctg
     6061 ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg gcagaagaag
     6121 gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt ggctctgtcc
     6181 aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac ggcctacctg
     6241 tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg ctttaaccag
     6301 tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa gaaagcaggg
     6361 aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc cggcacctac
     6421 gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc ggcccgccag
     6481 gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga cgtgatccta
     6541 ggtggaggcc gaaagtacat gtttcgcatg ggaaccccag accctgagta cccagatgac
     6601 tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg gctggcgaag
     6661 cgccagggtg cccggtatgt gtggaaccgc actgagctca tgcaggcttc cctggacccg
     6721 tctgtgaccc atctcatggg tctctttgag cctggagaca tgaaatacga gatccaccga
     6781 gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg cctgctgagc
     6841 aggaaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca tggtcatcat
     6901 gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc cattgagagg
     6961 gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga ccactcccac
     7021 gtcttctcct tcggaggcta ccccctgcga gggagctcca tcttcgggct ggcccctggc
     7081 aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc aggctatgtg
     7141 ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc cgagtatcgg
     7201 cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt ggcggtgttc
     7261 gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt catagcgcac
     7321 gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc gccccccgcc
     7381 ggcaccaccg acgccgcgca cccgggtggc ggaggactga acgacatctt cgaggctcag
     7441 aaaatcgaat ggcacgaaca tcaccatcac catcaccatc accattaagg ccggcctgta
     7501 gtcactggca caacgcgtta cccggtaagc caatcgggta tacacggtcg tcatactgca
     7561 gacagggttc ttctactttg caagatagtc ttgagtagta aaataaatag atagagaaaa
     7621 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     7681 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     7741 tcacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     7801 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     7861 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7921 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     7981 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8041 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8101 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     8161 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     8221 tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     8281 taatacgaca aggtgaggaa ctaaaccatg accgagtaca agcccacggt gcgcctcgcc
     8341 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     8401 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     8461 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     8521 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     8581 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     8641 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     8701 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     8761 gagcgcgccg gggtgcccgc cttcctggag acatccgcgc cccgcaacct ccccttctac
     8821 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     8881 tgcatgaccc gcaagcccgg tgcctgacac gtgctacgag atttcgattc caccgccgcc
     8941 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     9001 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     9061 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     9121 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     9181 tctagctaga gcttggcgta atcatggtca ttaccaatgc ttaatcagtg aggcacctat
     9241 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     9301 tacgatacgg gagggcttac catctggccc cagcgctgcg atgataccgc gagaaccacg
     9361 ctcaccggct ccggatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     9421 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9481 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc atcgctacag gcatcgtggt
     9541 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9601 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9661 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9721 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9781 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9841 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9901 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9961 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    10021 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tattcttcct
    10081 t
//
