LOCUS       sequence-304273-       10093 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10093)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-CNTN3-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10093
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5698..5733
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5743..5766
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7426..7470
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7471..7497
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7637..7654)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7643..7754
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7872..7892)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7877..8206
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8057..8192
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8119..8138
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8254..8301
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8320..8919
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8320..8339)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8816..8836
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9049..9170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9086..9105)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9140..9159
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9223..10014,10015..10083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9846..9865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10084..10093,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccga attgctgctg
     2761 caaggccctg tgttcatcaa agagcccagc aacagcatct tccccgtggg cagcgaggac
     2821 aagaagatca cactgcactg cgaggccaga ggcaacccat ctcctcacta tagatggcag
     2881 ctgaacggca gcgacatcga catgagcatg gaacaccggt acaagctgaa tggcggcaac
     2941 ctggtggtca tcaaccccaa cagaaactgg gacaccggca cctaccagtg cttcgccaca
     3001 aatagcctgg gcaccattgt gtccagagag gccaagctgc agttcgccta cctggaaaac
     3061 ttcaagacca agatgcggag caccgtgtcc gttagagaag gacaaggcgt ggtgctgctg
     3121 tgtggacctc ctcctcattc cggcgaactg tcctacgcct ggatcttcaa cgagtacccc
     3181 agcttcgtgg aagaggacag ccgcagattc gtgtcccaag agacaggcca cctgtacatc
     3241 agcaaggtgg aacccagcga cgtgggcaac tatacctgtg tggtcaccag catggtcacc
     3301 aacgccagag tgctgggatc tcctacacct ctggtgctga gatctgatgg cgtgatgggc
     3361 gagtacgagc ccaagatcga ggtgcagttt cctgaaacac tgcctgccgc caagggcagc
     3421 acagtgaagc tggaatgttt cgccctgggc aaccccattc cacagatcaa ttggcggaga
     3481 agcgacggcc tgcctttcag ctccaagatc aagctgagaa agttcagcgg cgtgctggaa
     3541 atccccaact tccagcaaga ggatgccggc agctatgagt gtatcgccga gaacagcaga
     3601 ggcaagaatg tggccagggg cagactgacc tactacgcca aacctcactg ggtgcagctg
     3661 atcaaggacg tggaaatcgc cgtggaagat agcctgtact gggagtgtag agccagcggc
     3721 aagcccaagc ctagctacag atggctgaaa aacggcgctg ccctggtgct ggaagagaga
     3781 acacagattg agaacggcgc cctgaccatc agcaacctgt ctgtgaccga tagcggcatg
     3841 ttccagtgca ttgccgaaaa caagcacggc ctggtgtaca gctctgccga actgaaagtg
     3901 gtggccagcg ctcccgactt cagcaagaac cccatgaaga aactggtgca ggtccaagtg
     3961 ggcagcctgg tgtctctgga ttgcaagcct agagcctctc ctagggctct gagcagctgg
     4021 aaaaaaggcg acgtgtccgt gcaagagcac gagagaatca gcctgctgaa cgacggcggc
     4081 ctgaagatcg ccaatgtgac aaaagccgac gccggcacat acacatgcat ggccgagaat
     4141 cagtttggca aggccaacgg cacaacccac ctggtcgtga ccgagcctac aagaatcaca
     4201 ctggccccta gcaacatgga tgtgtctgtg ggcgagagcg tgatcctgcc ttgtcaggtt
     4261 cagcacgacc ctctgctgga catcatcttc acctggtact tcaatggcgc cctggccgac
     4321 ttcaagaagg atggcagcca ctttgagaaa gtcggcggct ctagcagcgg cgacctgatg
     4381 atcagaaata tccagctgaa gcacagcggc aaatacgtgt gcatggtgca gacaggcgtg
     4441 gactctgtgt ctagcgccgc tgatctgatc gtcagaggat ctcccggacc tcctgagaac
     4501 gtgaaggtgg acgagatcac cgacacaaca gcccagctga gctggaaaga gggcaaagac
     4561 aatcacagcc ccgtgatcag ctacagcatc caggccagaa cacctttcag cgtcggctgg
     4621 cagacagtga ccacagtgcc tgaagtgatc gacggcaaga cccacacagc caccgtggtg
     4681 gaactgaacc cttgggtcga gtatgagttc cgcgtggtgg cctctaacaa gatcggaggc
     4741 ggagagccta gcctgcctag cgaaaaagtt cggactgaag aggccgtgcc tgaggtgcca
     4801 ccatctgaag ttaatggtgg cggcggaagc agaagcgagc tggttattac atgggacccc
     4861 gtgccagagg aactgcagaa tggcgaaggc tttggctacg tggtggcttt cagacctctg
     4921 ggcgtgacca cctggattca gacagtggtc acaagccccg acacacccag atacgtgttc
     4981 agaaacgaga gcatcgtgcc ttacagcccc tatgaagtga aagtgggcgt gtacaacaac
     5041 aaaggcgagg gcccttttag ccctgtgacc accgtgttta gcgccgaaga ggaacctaca
     5101 gtggcccctt ctcaggtgtc cgccaatagc ctgagcagca gcgagattga ggtgtcctgg
     5161 aacacaatcc cctggaagct gagcaacggc catctgctgg gatacgaagt gcggtattgg
     5221 aacggcggag gcaaagagga atccagcagc aagatgaagg tggccggcaa cgagacatct
     5281 gccagactga gaggcctgaa gtccaacctg gcctactata cagccgtgcg ggcctataat
     5341 tctgccggcg ctggaccatt cagcgccacc gtgaatgtga ccacaaagaa aacccctcca
     5401 agccagcctc ctggcaacgt cgtgtggaat gccaccgata ccaaagtgct gctgaactgg
     5461 gagcaagtga aggccatgga aaacgagtcc gaagtgaccg gctacaaagt gttctaccgg
     5521 accagcagcc agaacaacgt ccaggtgctg aacaccaaca agacctctgc agaactggtg
     5581 ctgcctatca aagaggacta catcatcgaa gtcaaggcca caacagatgg cggcgacggc
     5641 acatctagcg agcagatcag aatccccaga atcacctcca tggacgctgg cgcgccggaa
     5701 gatcaagttg atccaagatt gattgatggc aagggcggat ctctggaggt gctgttccag
     5761 ggaccaggcg gaagcggcaa tcccggcggc gatctggccc cccagatgct gcgcgagctg
     5821 caggagacca atgccgccct gcaggacgtg cgcgagctgc tgcgccaccg cgtgaaggag
     5881 atcaccttcc tgaagaacac cgtgatggag tgcgacgcct gcggtatgca gccctctaga
     5941 ggctccggct ccatcatccc agttgaggag gagaacccgg acttctggaa ccgcgaggca
     6001 gccgaggccc tgggtgccgc caagaagctg cagcctgcac agacagccgc caagaacctc
     6061 atcatcttcc tgggcgatgg gatgggggtg tctacggtga cagctgccag gatcctaaaa
     6121 gggcagaaga aggacaaact ggggcctgag atacccctgg ccatggaccg cttcccatat
     6181 gtggctctgt ccaagacata caatgtagac aaacatgtgc cagacagtgg agccacagcc
     6241 acggcctacc tgtgcggggt caagggcaac ttccagacca ttggcttgag tgcagccgcc
     6301 cgctttaacc agtgcaacac gacacgcggc aacgaggtca tctccgtgat gaatcgggcc
     6361 aagaaagcag ggaagtcagt gggagtggta accaccacac gagtgcagca cgcctcgcca
     6421 gccggcacct acgcccacac ggtgaaccgc aactggtact cggacgccga cgtgcctgcc
     6481 tcggcccgcc aggaggggtg ccaggacatc gctacgcagc tcatctccaa catggacatt
     6541 gacgtgatcc taggtggagg ccgaaagtac atgtttcgca tgggaacccc agaccctgag
     6601 tacccagatg actacagcca aggtgggacc aggctggacg ggaagaatct ggtgcaggaa
     6661 tggctggcga agcgccaggg tgcccggtat gtgtggaacc gcactgagct catgcaggct
     6721 tccctggacc cgtctgtgac ccatctcatg ggtctctttg agcctggaga catgaaatac
     6781 gagatccacc gagactccac actggacccc tccctgatgg agatgacaga ggctgccctg
     6841 cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg tcgcatcgac
     6901 catggtcatc atgaaagcag ggcttaccgg gcactgactg agacgatcat gttcgacgac
     6961 gccattgaga gggcgggcca gctcaccagc gaggaggaca cgctgagcct cgtcactgcc
     7021 gaccactccc acgtcttctc cttcggaggc taccccctgc gagggagctc catcttcggg
     7081 ctggcccctg gcaaggcccg ggacaggaag gcctacacgg tcctcctata cggaaacggt
     7141 ccaggctatg tgctcaagga cggcgcccgg ccggatgtta ccgagagcga gagcgggagc
     7201 cccgagtatc ggcagcagtc agcagtgccc ctggacgaag agacccacgc aggcgaggac
     7261 gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca ggagcagacc
     7321 ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc cctacaccgc ctgcgacctg
     7381 gcgccccccg ccggcaccac cgacgccgcg cacccgggtg gcggaggact gaacgacatc
     7441 ttcgaggctc agaaaatcga atggcacgaa catcaccatc accatcacca tcaccattaa
     7501 ggccggcctg tagtcactgg cacaacgcgt tacccggtaa gccaatcggg tatacacggt
     7561 cgtcatactg cagacagggt tcttctactt tgcaagatag tcttgagtag taaaataaat
     7621 agatagagaa aatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     7681 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     7741 gaggaaattg catcacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7801 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7861 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     7921 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     7981 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     8041 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8101 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     8161 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     8221 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     8281 atcggcatag tataatacga caaggtgagg aactaaacca tgaccgagta caagcccacg
     8341 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     8401 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     8461 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     8521 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     8581 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     8641 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     8701 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     8761 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacatccgc gccccgcaac
     8821 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     8881 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac acgtgctacg agatttcgat
     8941 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     9001 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     9061 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     9121 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     9181 ataccgtcga cctctagcta gagcttggcg taatcatggt cattaccaat gcttaatcag
     9241 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9301 cgtgtagata actacgatac gggagggctt accatctggc cccagcgctg cgatgatacc
     9361 gcgagaacca cgctcaccgg ctccggattt atcagcaata aaccagccag ccggaagggc
     9421 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9481 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccatcgctac
     9541 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9601 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9661 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9721 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9781 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9841 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9901 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9961 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10021 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10081 catattcttc ctt
//
