LOCUS       sequence-304275-       10108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-ADGRF5-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5713..5748
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5758..5781
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7441..7485
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7486..7512
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7652..7669)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7658..7769
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7887..7907)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7892..8221
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8072..8207
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8134..8153
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8269..8316
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8335..8934
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8335..8354)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8831..8851
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9064..9185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9101..9120)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9155..9174
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9238..10029,10030..10098))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9861..9880
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10099..10108,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccgc tctgaattgg
     2761 aactacgaga gcaccatcca tcctctgagc ctgcacgaac atgagcctgc tggcgaagag
     2821 gccctgagac agaaaagagc cgtggccaca aagagcccca ccgccgaaga gtacaccgtg
     2881 aacatcgaga tcagcttcga gaacgccagc tttctggacc ccatcaaggc ctacctgaac
     2941 agcctgagct tccccatcca cggcaacaac accgaccaga tcaccgacat cctgagcatc
     3001 aacgtgacca ccgtgtgcag acctgccggc aatgagatct ggtgtagctg tgaaacaggc
     3061 tacggctggc ccagagagag atgcctgcac aacctgatct gccaagagcg ggatgtgttt
     3121 ctgcctggcc accactgcag ctgcctgaaa gaactgcctc ctaatggccc cttctgcctg
     3181 ctgcaagagg acgtgaccct gaacatgaga gtgcggctga acgtgggctt ccaagaggac
     3241 ctgatgaata ccagcagcgc cctgtacaga agctacaaga ccgacctgga aaccgccttc
     3301 cggaaaggct atggcatcct gcctggcttt aagggcgtga ccgtgacagg ctttaagagc
     3361 ggcagcgtgg tggtcaccta cgaagtgaaa accacacctc ctagcctgga actgatccac
     3421 aaggccaacg aacaggtggt gcagagcctg aaccagacct acaagatgga ctacaacagc
     3481 ttccaggccg tgaccatcaa cgagagcaat ttcttcgtga ccccggaaat catcttcgag
     3541 ggcgacacag tgtccctcgt gtgcgagaaa gaggtgctgt ctagtaacgt gtcctggcgc
     3601 tacgaggaac agcagctgga aatccagaac agctcccggt tcagcatcta cacagccctg
     3661 ttcaacaaca tgaccagcgt gtccaagctg accatccaca acattacacc tggcgacgcc
     3721 ggcgagtatg tgtgcaagct gatcctggat atcttcgagt acgagtgcaa gaaaaagatc
     3781 gacgtgatgc ccatccagat cctcgccaac gaagagatga aggtcatgtg cgacaacaac
     3841 cccgtgtctc tgaactgctg cagccagggc aatgtgaact ggtccaaggt ggaatggaag
     3901 caagagggca agatcaacat ccccggcaca cccgagacag acatcgactc cagctgtagc
     3961 agatacaccc tgaaggccga tggcacccag tgtcctagcg gatcttctgg caccaccgtg
     4021 atctacacat gcgagttcat cagcgcctac ggcgccagag gcagcgccaa tatcaaagtg
     4081 acctttatct ccgtggccaa cctcaccatc acacccgatc ctatctctgt gtccgagggc
     4141 cagaacttca gcatcaagtg catcagcgac gtgtccaact acgacgaggt gtactggaat
     4201 acctccgccg gcatcaagat ctaccagcgg ttctacacca ccagaagata cctggacggc
     4261 gccgagagcg tgctgaccgt gaaaacaagc acaagagagt ggaacggcac ctaccactgc
     4321 atcttccggt acaagaacag ctactctatc gccaccaagg acgtgatcgt gcaccctctg
     4381 cctctgaagc tgaacatcat ggtggaccct ctggaagcca cagtgtcttg ttctggctcc
     4441 caccacatca agtgttgcat cgaagaggac ggcgactaca aagtgacatt ccacaccggc
     4501 tcctcttctc tgcctgccgc caaagaagtg aacaagaagc aagtctgcta taagcacaac
     4561 ttcaacgcct cttccgtgtc atggtgcagc aagaccgtgg acgtgtgctg ccactttacc
     4621 aacgccgcca acaatagcgt gtggtcccct agcatgaagc tcaacctggt gcctggcgag
     4681 aacatcacct gtcaggaccc tgtgattggc gtgggcgagc ctggaaaagt gatccagaag
     4741 ctgtgcagat tcagcaacgt gccaagcagc cctgagtctc ctatcggcgg caccatcacc
     4801 tataagtgtg tgggcagcca gtgggaagag aagcggaacg attgcatctc tgcccctatc
     4861 aactccctgc tgcagatggc caaggcactg atcaagagcc ctagccagga tgagatgctg
     4921 cccacctatc tgaaggacct gtccatcagc atcgacaagg ccgagcacga gatctcttct
     4981 agccctggat ctctgggcgc catcatcaat atcctggacc tgctgagcac cgtgcctaca
     5041 caagtgaact ccgagatgat gacccacgtg ctgtccaccg tgaatgtgat cctgggcaag
     5101 cccgtgctga acacctggaa agttctccag cagcagtgga ccaaccagtc ctctcagctg
     5161 ctgcacagcg tggaaagatt ctctcaggcc ctgcagtctg gcgatagccc tccactgagc
     5221 ttcagccaga ccaacgtgca gatgagcagc atggtcatca agagcagtca ccccgaaacc
     5281 taccagcaga gattcgtgtt cccctacttc gacctgtggg gcaacgtcgt gatcgacaag
     5341 agctatctgg aaaacctgca gagcgacagc agcatcgtga ccatggcctt tccaacactg
     5401 caggccattc tggcccagga catccaagag aacaactttg ccgagagcct ggtcatgaca
     5461 accactgtgt cccacaacac caccatgcca ttccggatca gcatgacctt taagaacaac
     5521 agcccctctg gcggcgagac taagtgcgtg ttctggaact tccggctggc caacaacaca
     5581 ggcggctggg attctagcgg ctgctatgtt gaagaaggcg acggcgataa cgtgacctgc
     5641 atctgtgatc acctgaccag cttctccatc ctgatgagcc ctgacagccc cgatccaagc
     5701 tctggcgcgc cggaagatca agttgatcca agattgattg atggcaaggg cggatctctg
     5761 gaggtgctgt tccagggacc aggcggaagc ggcaatcccg gcggcgatct ggccccccag
     5821 atgctgcgcg agctgcagga gaccaatgcc gccctgcagg acgtgcgcga gctgctgcgc
     5881 caccgcgtga aggagatcac cttcctgaag aacaccgtga tggagtgcga cgcctgcggt
     5941 atgcagccct ctagaggctc cggctccatc atcccagttg aggaggagaa cccggacttc
     6001 tggaaccgcg aggcagccga ggccctgggt gccgccaaga agctgcagcc tgcacagaca
     6061 gccgccaaga acctcatcat cttcctgggc gatgggatgg gggtgtctac ggtgacagct
     6121 gccaggatcc taaaagggca gaagaaggac aaactggggc ctgagatacc cctggccatg
     6181 gaccgcttcc catatgtggc tctgtccaag acatacaatg tagacaaaca tgtgccagac
     6241 agtggagcca cagccacggc ctacctgtgc ggggtcaagg gcaacttcca gaccattggc
     6301 ttgagtgcag ccgcccgctt taaccagtgc aacacgacac gcggcaacga ggtcatctcc
     6361 gtgatgaatc gggccaagaa agcagggaag tcagtgggag tggtaaccac cacacgagtg
     6421 cagcacgcct cgccagccgg cacctacgcc cacacggtga accgcaactg gtactcggac
     6481 gccgacgtgc ctgcctcggc ccgccaggag gggtgccagg acatcgctac gcagctcatc
     6541 tccaacatgg acattgacgt gatcctaggt ggaggccgaa agtacatgtt tcgcatggga
     6601 accccagacc ctgagtaccc agatgactac agccaaggtg ggaccaggct ggacgggaag
     6661 aatctggtgc aggaatggct ggcgaagcgc cagggtgccc ggtatgtgtg gaaccgcact
     6721 gagctcatgc aggcttccct ggacccgtct gtgacccatc tcatgggtct ctttgagcct
     6781 ggagacatga aatacgagat ccaccgagac tccacactgg acccctccct gatggagatg
     6841 acagaggctg ccctgcgcct gctgagcagg aacccccgcg gcttcttcct cttcgtggag
     6901 ggtggtcgca tcgaccatgg tcatcatgaa agcagggctt accgggcact gactgagacg
     6961 atcatgttcg acgacgccat tgagagggcg ggccagctca ccagcgagga ggacacgctg
     7021 agcctcgtca ctgccgacca ctcccacgtc ttctccttcg gaggctaccc cctgcgaggg
     7081 agctccatct tcgggctggc ccctggcaag gcccgggaca ggaaggccta cacggtcctc
     7141 ctatacggaa acggtccagg ctatgtgctc aaggacggcg cccggccgga tgttaccgag
     7201 agcgagagcg ggagccccga gtatcggcag cagtcagcag tgcccctgga cgaagagacc
     7261 cacgcaggcg aggacgtggc ggtgttcgcg cgcggcccgc aggcgcacct ggttcacggc
     7321 gtgcaggagc agaccttcat agcgcacgtc atggccttcg ccgcctgcct ggagccctac
     7381 accgcctgcg acctggcgcc ccccgccggc accaccgacg ccgcgcaccc gggtggcgga
     7441 ggactgaacg acatcttcga ggctcagaaa atcgaatggc acgaacatca ccatcaccat
     7501 caccatcacc attaaggccg gcctgtagtc actggcacaa cgcgttaccc ggtaagccaa
     7561 tcgggtatac acggtcgtca tactgcagac agggttcttc tactttgcaa gatagtcttg
     7621 agtagtaaaa taaatagata gagaaaatca gcctcgactg tgccttctag ttgccagcca
     7681 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     7741 ctttcctaat aaaatgagga aattgcatca caacactcaa ccctatctcg gtctattctt
     7801 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7861 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     7921 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     7981 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8041 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8101 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8161 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     8221 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     8281 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatgacc
     8341 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     8401 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     8461 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     8521 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     8581 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     8641 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     8701 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     8761 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagaca
     8821 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     8881 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacacgtg
     8941 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     9001 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     9061 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9121 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9181 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatta
     9241 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9301 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9361 cgctgcgatg ataccgcgag aaccacgctc accggctccg gatttatcag caataaacca
     9421 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9481 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9541 tgttgccatc gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9601 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9661 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9721 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9781 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9841 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9901 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9961 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10021 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10081 gaaatgttga atactcatat tcttcctt
//
