LOCUS       sequence-304277-       10111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-IGSF10(Trunc2)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5716..5751
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5761..5784
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7444..7488
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7489..7515
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7655..7672)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7661..7772
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7890..7910)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7895..8224
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8075..8210
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8137..8156
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8272..8319
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8338..8937
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8338..8357)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8834..8854
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9067..9188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9104..9123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9158..9177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9241..10032,10033..10101))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9864..9883
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10102..10111,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccgt gaaaatgaag
     2761 ggccaaagac ctctggaaca cgacggcgag acagaaggct ctggactgga cgagagcaac
     2821 cctatcgctc acctgaaaga gcctccaggc gcacagctga gaacaagcgc tctgatggaa
     2881 gccgaagtgg gcaagcacac cagcagcacc agcaagcggc acaactacag agagctgacc
     2941 ctgcagagaa gaggcgacag cacccacaga cggttcagag agaacagacg gcacttccca
     3001 cctagcgcca gaagaatcga tccccagcat tgggccgctc tgctggaaaa ggccaagaaa
     3061 aacgccatgc ctgacaagcg cgagaacacc accgtttctc cacctccagt ggtcacccag
     3121 ctgcctaaca ttcccggcga agaggatgac agctctggca tgctggccct gcacgaggaa
     3181 ttcatggtgc ctgccacaaa ggccctgaac ctgcctgcca gaacagtgac cgccgacagc
     3241 agaaccatca gcgacagccc catgaccaac atcaactacg gcaccgagtt cagccccgtg
     3301 gtcaactctc agatcctgcc tcctgaggaa cccaccgact tcaagctgag caccgccatc
     3361 aagaccaccg ccatgagcaa gaacatcaac cccaccatga gcagccagat ccagggcacc
     3421 acaaaccagc acagcagcac agtgttccct ctgctgctgg gcgccaccga gtttcaggat
     3481 agcgatcaga tgggcagagg cagagagcac ttccagagca gacctccaat caccgtgcgg
     3541 accatgatca aggacgtgaa cgtgaagatg ctgtccagca ccaccaacaa actgctgctc
     3601 gagtccgtga ataccaccaa tagccaccag accagcgtgc gggaagtgtc cgagcctaga
     3661 cacaaccact tctacagcca caccacacag atcctgtcca ccagcacatt ccccagcgat
     3721 cctcacacag ccgctcactc tcagttcccc attcctcgga actctaccgt gaacatccct
     3781 ctgttcagaa gattcggccg gcagagaaag atcggcggca ggggcagaat catctcccca
     3841 tacagaaccc ctgtgctgcg gcggcaccgg tactccatct ttagaagcac cacacggggc
     3901 agcagcgaga agtctaccac agcctttagc gccaccgtgc tgaacgtgac ctgtctgagc
     3961 tgtctgccca gagagagact gaccacagcc acagctgccc tgagctttcc atctgccgct
     4021 cctatcacct ttcctaaggc cgatatcgcc agagtgccca gcgaggaatc tacaaccctg
     4081 gtgcagaatc cactgctgct gcttgagaac aaacccagcg tggaaaagac cacacctacc
     4141 atcaagtact tccggaccga gatcagccaa gtgaccccaa caggcgccgt gatgacatac
     4201 gcccctacca gcattcccat ggaaaaaacc cacaaagtga acgccagcta tccccgggtg
     4261 tcctccacaa atgaggccaa gagggacagc gtgatcacct ctagtctgag cggcgccatc
     4321 accaagcctc caatgaccat cattgccatc acacggttca gccggcggaa gatcccttgg
     4381 cagcagaact tcgtgaacaa tcacaacccc aagggcagac tgcggaatca gcacaaggtg
     4441 tccctgcaga aaagcacagc cgtgatgctg cccaagacaa gccctgctct gcctcgggac
     4501 aaagtgtccc ctttccactt caccacactg agcaccagcg tgatgcagat ccctagcaac
     4561 accctgacca ccgctcacca caccaccaca aagacccaca atcctggcag cctgcctacc
     4621 aagaaagagc tgccctttcc tcctctgaac cccatgctgc cctccatcat cagcaaggac
     4681 agctccacca agagcatcat ctctacccag accgccattc ctgccaccac tcctacattt
     4741 cccgcctccg tgattaccta cgagacacag accgagcgga gcagagccca gaccattcag
     4801 cgggaacaag agccccagaa gaagaaccgg accgatccta acatcagccc cgatcagtcc
     4861 agcggcttca caacacccac agccatgaca ccacctgtgc tgacaacagc cgagacaagc
     4921 gtgaagccta gcgtgtccgc cttcacacac agccctccag agaacacaac cggcatctct
     4981 agcaccatca gcttccacag caggaccctg aatctgaccg acgtgatcga ggaactggcc
     5041 caggcctcta cacagaccct gaagtctaca atcgccagcg agacaaccct gagcagcaag
     5101 agccaccagt ccaccacaac cagaaaggcc atcatccggc actctacaat ccctccattc
     5161 ctgtccagct ccgccacact gatgcccgtg cctatcagcc cacctttcac acagagagcc
     5221 gtgaccgaca acgtggccac acctatctct ggcctgatga ccaataccgt cgtgaagctg
     5281 cacgagagca gccggcacaa tgccaaacct cagcagctgg tggcagaggt ggccacatct
     5341 cctaaggtgc accccaacgc caagttcacc atcggcacca cacacttcat ctacagcaac
     5401 ctgctgcaca gcacccctat gcctgctctg acaaccgtga agtcccagaa cagcaagctg
     5461 accccatctc cttgggccga gaaccagttc tggcacaagc cctatagcga gatcgccgag
     5521 aagggcaaga aacccgaggt gtcaatgctg gccacaacag gactgagcga ggccacaact
     5581 ctggtgtccg attgggacgg acagaagaac accaagaagt ccgacttcga caaaaagccc
     5641 gtgcaagagg ccactacctc caagctgctg cctttcgata gcctgagccg gtacatcttc
     5701 gagaaaggcg cgccggaaga tcaagttgat ccaagattga ttgatggcaa gggcggatct
     5761 ctggaggtgc tgttccaggg accaggcgga agcggcaatc ccggcggcga tctggccccc
     5821 cagatgctgc gcgagctgca ggagaccaat gccgccctgc aggacgtgcg cgagctgctg
     5881 cgccaccgcg tgaaggagat caccttcctg aagaacaccg tgatggagtg cgacgcctgc
     5941 ggtatgcagc cctctagagg ctccggctcc atcatcccag ttgaggagga gaacccggac
     6001 ttctggaacc gcgaggcagc cgaggccctg ggtgccgcca agaagctgca gcctgcacag
     6061 acagccgcca agaacctcat catcttcctg ggcgatggga tgggggtgtc tacggtgaca
     6121 gctgccagga tcctaaaagg gcagaagaag gacaaactgg ggcctgagat acccctggcc
     6181 atggaccgct tcccatatgt ggctctgtcc aagacataca atgtagacaa acatgtgcca
     6241 gacagtggag ccacagccac ggcctacctg tgcggggtca agggcaactt ccagaccatt
     6301 ggcttgagtg cagccgcccg ctttaaccag tgcaacacga cacgcggcaa cgaggtcatc
     6361 tccgtgatga atcgggccaa gaaagcaggg aagtcagtgg gagtggtaac caccacacga
     6421 gtgcagcacg cctcgccagc cggcacctac gcccacacgg tgaaccgcaa ctggtactcg
     6481 gacgccgacg tgcctgcctc ggcccgccag gaggggtgcc aggacatcgc tacgcagctc
     6541 atctccaaca tggacattga cgtgatccta ggtggaggcc gaaagtacat gtttcgcatg
     6601 ggaaccccag accctgagta cccagatgac tacagccaag gtgggaccag gctggacggg
     6661 aagaatctgg tgcaggaatg gctggcgaag cgccagggtg cccggtatgt gtggaaccgc
     6721 actgagctca tgcaggcttc cctggacccg tctgtgaccc atctcatggg tctctttgag
     6781 cctggagaca tgaaatacga gatccaccga gactccacac tggacccctc cctgatggag
     6841 atgacagagg ctgccctgcg cctgctgagc aggaaccccc gcggcttctt cctcttcgtg
     6901 gagggtggtc gcatcgacca tggtcatcat gaaagcaggg cttaccgggc actgactgag
     6961 acgatcatgt tcgacgacgc cattgagagg gcgggccagc tcaccagcga ggaggacacg
     7021 ctgagcctcg tcactgccga ccactcccac gtcttctcct tcggaggcta ccccctgcga
     7081 gggagctcca tcttcgggct ggcccctggc aaggcccggg acaggaaggc ctacacggtc
     7141 ctcctatacg gaaacggtcc aggctatgtg ctcaaggacg gcgcccggcc ggatgttacc
     7201 gagagcgaga gcgggagccc cgagtatcgg cagcagtcag cagtgcccct ggacgaagag
     7261 acccacgcag gcgaggacgt ggcggtgttc gcgcgcggcc cgcaggcgca cctggttcac
     7321 ggcgtgcagg agcagacctt catagcgcac gtcatggcct tcgccgcctg cctggagccc
     7381 tacaccgcct gcgacctggc gccccccgcc ggcaccaccg acgccgcgca cccgggtggc
     7441 ggaggactga acgacatctt cgaggctcag aaaatcgaat ggcacgaaca tcaccatcac
     7501 catcaccatc accattaagg ccggcctgta gtcactggca caacgcgtta cccggtaagc
     7561 caatcgggta tacacggtcg tcatactgca gacagggttc ttctactttg caagatagtc
     7621 ttgagtagta aaataaatag atagagaaaa tcagcctcga ctgtgccttc tagttgccag
     7681 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     7741 gtcctttcct aataaaatga ggaaattgca tcacaacact caaccctatc tcggtctatt
     7801 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     7861 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     7921 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     7981 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8041 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     8101 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     8161 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     8221 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     8281 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     8341 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     8401 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     8461 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     8521 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     8581 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     8641 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     8701 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     8761 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     8821 acatccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     8881 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacac
     8941 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     9001 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     9061 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9121 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9181 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     9241 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9301 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9361 cagcgctgcg atgataccgc gagaaccacg ctcaccggct ccggatttat cagcaataaa
     9421 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9481 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9541 cgttgttgcc atcgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9601 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9661 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9721 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9781 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9841 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9901 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9961 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10021 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10081 acggaaatgt tgaatactca tattcttcct t
//
