LOCUS       sequence-30428-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   He L, Olson DP, Wu X, Karpova TS, McNally JG, Lipsky PE
  TITLE     A flow cytometric method to detect protein-protein interaction in 
            living cells by directly visualizing donor fluorophore quenching 
            during CFP-->YFP fluorescence resonance energy transfer (FRET).
  JOURNAL   Cytometry A. 2003 Oct . 55(2):71-85.
  PUBMED    14505312
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cytometry 
            A. 2003 Oct . 55(2):71-85."
COMMENT     Sequence Label: pCMV-ECFPEYFP
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    132..188
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(189..440)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(231..252)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     580..599
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aaacagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
       61 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagttat
      121 ctagatccgg tggatcccgg gcccgcggta ccgtcgactg cagaattcga agcttgagct
      181 cgagatctct tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
      241 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctggtagctc
      301 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
      361 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg
      421 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
      481 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
      541 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
      601 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
      661 tgctgcttca tgtggtcggg gtagcgggcg aagcactgca ggccgtagcc gaaggtggtc
      721 acga
//
