LOCUS       sequence-304281-       10210 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein partial extracellular 
            domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-SDK1(Trunc3)-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5815..5850
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5860..5883
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7543..7587
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7588..7614
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7754..7771)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7760..7871
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7989..8009)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7994..8323
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8174..8309
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8236..8255
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8371..8418
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8437..9036
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8437..8456)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8933..8953
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9166..9287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9203..9222)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9257..9276
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9340..10131,10132..10200))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9963..9982
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10201..10210,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccgc tgttggacac
     2761 ctgagcttta cagagatcct ggacaccagc ctgaaggtgt cctggcaaga gcccctggaa
     2821 aagaacggca tcatcaccgg ctaccagatc agctgggaag tgtacggcag aaacgacagc
     2881 agactgaccc acacactgaa cagcaccaca cacgagtaca agatccaggg cctgagcagc
     2941 ctgaccacct acacaattga tgtggccgcc gtgacagccg ttggcacagg actggtcaca
     3001 agcagcacaa tctctagcgg cgtgccacct gatttgcctg gcgctcctag caacctggtc
     3061 atcagcaaca tcagccccag aagcgccaca ctgcagttca gaccaggcta cgatggcaag
     3121 accagcatca gcagatggat cgtggaaggc caagtgggag ccatcggaga tgaggaagag
     3181 tgggtcaccc tgtacgagga agagaacgag cctgacgctc agatgctgga aatccccaat
     3241 ctgacccctt acacacacta ccggttccgg atgaagcaag tgaacatcgt gggcccatct
     3301 ccatacagcc ccagcagcag agtgattcag acactgcagg ctcctccaga tgtggcccct
     3361 acaagcgtga cagtgcggac agcctctgag acaagcctga gactgagatg ggtgcccctg
     3421 cctgatagcc agtacaacgg caatcctgag agcgtgggct acagaatcaa gtactggcgg
     3481 agcgatctgc agtctagcgc tgtggctcag gtggtgtctg accggctgga aagagagttc
     3541 accatcgagg aactggaaga atggatggaa tacgagctgc agatgcaggc cttcaatgcc
     3601 gttggagctg gaccttggag cgaggttgtc agaggcagaa ccagagaaag cgtgccatct
     3661 gccgctccag agaatgtgtc tgccgaagcc gtgtctagca cccagatcct gctgacctgg
     3721 acatccgtgc cagagcagga tcagaatggc ctgatcctgg gctataagat cctgttcaga
     3781 gccaaggatc tggaccccga gcctagatct cacatcgtgc ggggcaatca cacacagtct
     3841 gctctgctgg ccggactgag aaagttcgtg ctgtatgagc tgcaggtcct ggcctttacc
     3901 agaatcggaa atggcgtgcc cagcacaccc ctgattctgg aaagaacaaa ggacgacgcc
     3961 cctggacctc ctgtcagact ggtttttcct gaagtgcggc tgaccagcgt gcggattgtg
     4021 tggcaacctc ctgaggaacc caacggaatc atcctgggat accagatcgc ctacagactg
     4081 gccagcagct cccctcacac ctttacaaca gtggaagtgg gcgccaccgt gcggcagttt
     4141 acagctacag atctggctcc cgagagcgcc tacatcttca gactgagcgc caagacaaga
     4201 caaggctggg gagaacctct ggaagccacc gtgatcacca ccgagaagag agaaagaccc
     4261 gctcctccta gagaactgct ggtgcctcag gccgaagtga ccgctagatc tctgagactt
     4321 cagtgggtgc caggctctga tggcgcctct cctatccggt acttcaccat gcaagtgcgc
     4381 gagctgccta gaggcgagtg gcagacatac agcagctcta tctctcacga ggccaccgcc
     4441 tgtgtggtgg atagactgag gcctttcacc agctacaagc tgcggctgaa ggccaccaac
     4501 gacatcggcg atagcgactt cagcagcgag acagaggcag tgacaaccct gcaggatgtt
     4561 cctggcgagc ctcctggatc tgtgtccgcc acacctcaca caacaagcag cgttctgatc
     4621 cagtggcagc ctcctaggga tgagagcctg aatggactgc tgcagggcta tagaatctac
     4681 taccgcgagc tggaatatga ggccggcagc ggaacagagg ccaagacact gaagaaccct
     4741 atcgctctgc acgccgagct gacagcccag agcagcttta agaccgtgaa cagcagcagc
     4801 acctccacca tgtgcgagct gacccacctg aagaagtatc ggagatacga agtgatcatg
     4861 accgcctaca acatcatcgg cgagagccct gcttctgccc ctgtggaagt gtttgtggga
     4921 gaagccgctc ctgccatggc tccccagaat gtgcaagtga cacctctgac agccagccag
     4981 ctggaagtta catgggaccc tccaccacca gagagccaga acggcaatat tcagggctac
     5041 aagatctact attgggaagc cgacagccag aatgagacag agaagatgaa ggtgctgttt
     5101 ctgcccgagc ctgtcgtgcg cctgaagaat ctgaccagcc acacaaagta cctggtgtct
     5161 atcagcgcct tcaacgctgc tggcgacggc cctaaatctg atcctcagca gggaagaaca
     5221 caccaggcag ctccaggcgc tccaagcttt ctggcctttt ccgagatcac cagcaccact
     5281 ctcaatgtgt cttggggaga gcctgccgcc gctaatggca ttctgcaggg atacagagtg
     5341 gtgtacgagc ctctggctcc agtgcagggc gtgtccaaag tggtcaccgt ggaagttcgc
     5401 ggcaactggc agagatggct gaaagtgcgg gatctgacca agggcgtgac ctacttcttt
     5461 cgggtgcaag ccagaaccat cacatacgga cccgaactgc aggccaatat cacagctgga
     5521 ccagccgaag gatctccagg cagccctaga gatgtcctcg tgacaaagag cgccagcgaa
     5581 ctgaccctgc agtggacaga aggacacagc ggcgataccc ctaccacagg ctatgtgatc
     5641 gaggccagac cttctgacga aggcctgtgg gacatgttcg tgaaggacat ccctcggagc
     5701 gccacaagct acaccctgag cctggataag ctgagacagg gcgtcacata cgagtttaga
     5761 gtggtggccg tgaacgaggc cggctacggc gaaccttcta atcctggcgc gccggaagat
     5821 caagttgatc caagattgat tgatggcaag ggcggatctc tggaggtgct gttccaggga
     5881 ccaggcggaa gcggcaatcc cggcggcgat ctggcccccc agatgctgcg cgagctgcag
     5941 gagaccaatg ccgccctgca ggacgtgcgc gagctgctgc gccaccgcgt gaaggagatc
     6001 accttcctga agaacaccgt gatggagtgc gacgcctgcg gtatgcagcc ctctagaggc
     6061 tccggctcca tcatcccagt tgaggaggag aacccggact tctggaaccg cgaggcagcc
     6121 gaggccctgg gtgccgccaa gaagctgcag cctgcacaga cagccgccaa gaacctcatc
     6181 atcttcctgg gcgatgggat gggggtgtct acggtgacag ctgccaggat cctaaaaggg
     6241 cagaagaagg acaaactggg gcctgagata cccctggcca tggaccgctt cccatatgtg
     6301 gctctgtcca agacatacaa tgtagacaaa catgtgccag acagtggagc cacagccacg
     6361 gcctacctgt gcggggtcaa gggcaacttc cagaccattg gcttgagtgc agccgcccgc
     6421 tttaaccagt gcaacacgac acgcggcaac gaggtcatct ccgtgatgaa tcgggccaag
     6481 aaagcaggga agtcagtggg agtggtaacc accacacgag tgcagcacgc ctcgccagcc
     6541 ggcacctacg cccacacggt gaaccgcaac tggtactcgg acgccgacgt gcctgcctcg
     6601 gcccgccagg aggggtgcca ggacatcgct acgcagctca tctccaacat ggacattgac
     6661 gtgatcctag gtggaggccg aaagtacatg tttcgcatgg gaaccccaga ccctgagtac
     6721 ccagatgact acagccaagg tgggaccagg ctggacggga agaatctggt gcaggaatgg
     6781 ctggcgaagc gccagggtgc ccggtatgtg tggaaccgca ctgagctcat gcaggcttcc
     6841 ctggacccgt ctgtgaccca tctcatgggt ctctttgagc ctggagacat gaaatacgag
     6901 atccaccgag actccacact ggacccctcc ctgatggaga tgacagaggc tgccctgcgc
     6961 ctgctgagca ggaacccccg cggcttcttc ctcttcgtgg agggtggtcg catcgaccat
     7021 ggtcatcatg aaagcagggc ttaccgggca ctgactgaga cgatcatgtt cgacgacgcc
     7081 attgagaggg cgggccagct caccagcgag gaggacacgc tgagcctcgt cactgccgac
     7141 cactcccacg tcttctcctt cggaggctac cccctgcgag ggagctccat cttcgggctg
     7201 gcccctggca aggcccggga caggaaggcc tacacggtcc tcctatacgg aaacggtcca
     7261 ggctatgtgc tcaaggacgg cgcccggccg gatgttaccg agagcgagag cgggagcccc
     7321 gagtatcggc agcagtcagc agtgcccctg gacgaagaga cccacgcagg cgaggacgtg
     7381 gcggtgttcg cgcgcggccc gcaggcgcac ctggttcacg gcgtgcagga gcagaccttc
     7441 atagcgcacg tcatggcctt cgccgcctgc ctggagccct acaccgcctg cgacctggcg
     7501 ccccccgccg gcaccaccga cgccgcgcac ccgggtggcg gaggactgaa cgacatcttc
     7561 gaggctcaga aaatcgaatg gcacgaacat caccatcacc atcaccatca ccattaaggc
     7621 cggcctgtag tcactggcac aacgcgttac ccggtaagcc aatcgggtat acacggtcgt
     7681 catactgcag acagggttct tctactttgc aagatagtct tgagtagtaa aataaataga
     7741 tagagaaaat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     7801 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     7861 gaaattgcat cacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7921 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7981 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     8041 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     8101 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     8161 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8221 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     8281 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     8341 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     8401 ggcatagtat aatacgacaa ggtgaggaac taaaccatga ccgagtacaa gcccacggtg
     8461 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     8521 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     8581 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     8641 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     8701 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     8761 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     8821 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     8881 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga catccgcgcc ccgcaacctc
     8941 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     9001 cgcacctggt gcatgacccg caagcccggt gcctgacacg tgctacgaga tttcgattcc
     9061 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     9121 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     9181 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9241 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     9301 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat taccaatgct taatcagtga
     9361 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9421 gtagataact acgatacggg agggcttacc atctggcccc agcgctgcga tgataccgcg
     9481 agaaccacgc tcaccggctc cggatttatc agcaataaac cagccagccg gaagggccga
     9541 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9601 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca tcgctacagg
     9661 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9721 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9781 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9841 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9901 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9961 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10021 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10081 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10141 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10201 attcttcctt
//
