LOCUS       sequence-304289-       10306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-CNTN5-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5911..5946
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5956..5979
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7639..7683
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7684..7710
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(7850..7867)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7856..7967
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8085..8105)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8090..8419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8270..8405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8332..8351
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8467..8514
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8533..9132
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8533..8552)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9029..9049
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9262..9383
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9299..9318)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9353..9372
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9436..10227,10228..10296))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10059..10078
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10297..10306,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccga gtattctaag
     2761 tctctgcctg gcctgagcac cagctatgcc gctctgctga gaatcaagaa gtccagcagc
     2821 agctccctgt tcggcagcaa gaccagacct agatacagca gccctagcct gggcaccctg
     2881 tctgcctctt ctccatcttg gcttggcgcc gctcagaact actacagccc catcaacctg
     2941 taccacagca gcgacgcctt caagcaggat gagtccgtgg attacggccc cgtgttcgtg
     3001 caagagcccg acgacatcat cttccccacc gacagcgacg agaagaaggt ggccctgaat
     3061 tgcgaagtgc ggggcaatcc tgtgcctagc taccggtggc tgagaaacgg cacagagatc
     3121 gacctggaaa gcgactaccg gtacagcctg atcgacggca ccttcatcat cagcaacccc
     3181 agcgaggcca aggacagcgg ccattatcag tgcctggcca ccaataccgt gggcagcatt
     3241 ctgagcagag aggccacact gcagttcgcc tacctgggca atttcagcgg cagaaccaga
     3301 tccgccgtgt ccgtcagaga aggacaaggc gtggtgctga tgtgcagccc tccacctcac
     3361 agccccgaga tcatctacag ctgggtgttc aacgagttcc ccagcttcgt ggccgaggac
     3421 agcagacggt tcatcagcca agagacaggc aatctgtaca tcagcaaggt gcagaccagc
     3481 gacgtgggct cctacatctg cctggtcaag aacaccgtga ccaacgccag agtgctgagc
     3541 cctcctacac ctctgacact gagaaatgac ggcgtgatgg gcgagtacga gcccaagatc
     3601 gaggtgcact tcccctttac tgtgaccgcc gccaagggca ccaccgtgaa gatggaatgt
     3661 ttcgccctgg gcaaccccgt gcctaccatc acatggatga aggtcaacgg ctacatcccc
     3721 agcaaggccc gcctgagaaa atctcaggcc gtgctggaaa tccccaacgt gcagctggat
     3781 gacgccggca tctatgagtg cagagccgag aactcccggg gcaagaatag cttcagaggc
     3841 cagctgcagg tctacacata ccctcactgg gtcgagaagc tgaacgatac ccagctggat
     3901 agcggcagcc ctctgagatg ggagtgtaaa gccaccggca agcccagacc aacctacaga
     3961 tggctgaaga acggcgtgcc actgagccca cagagcagag tggaaatggt caatggcgtc
     4021 ctgatgatcc acaacgtgaa ccagagcgac gccggaatgt accagtgtct ggccgagaac
     4081 aaatacggcg ccatctacgc ctctgccgag ctgaagattc tggccagcgc tcccacattc
     4141 gccctgaacc agctgaagaa aaccatcatc gtgaccaagg accaagaggt ggtcatcgag
     4201 tgcaagcctc agggcagccc taagccaacc atcagctgga agaaaggcga cagagccgtg
     4261 cgcgagaaca agcggattgc catcctgcct gatggcagcc tgagaatcct gaacgccagc
     4321 aagagcgacg agggcaaata cgtgtgcagg ggcgagaatg tgtttggcag cgccgagatt
     4381 atcgccagcc tgagcgtgaa agagcccacc agaatcgagc tgacccctaa gagaacagag
     4441 ctgaccgtgg gcgagagcat cgtgctgaat tgcaaggcca tccacgatgc cagcctggac
     4501 gtgacctttt actggaccct gaagggccag cctatcgact tcgaggaaga aggcggacac
     4561 ttcgagtcca tcagagccca ggcctctagc gccgatctga tgatcagaaa tatcctgctg
     4621 atgcacgccg gaagatacgg ctgcagagtg cagacaacag ccgacagcgt gtccgatgaa
     4681 gccgagctgc ttgttagagg ccctcctgga ccaccaggca tcgtgatcgt ggaagagatc
     4741 accgagagca ccgccacact gtcttggagc cctgccgccg ataatcacag ccctatcagc
     4801 agctacaacc tgcaggccag atctcccttt agcctcggct ggcagaccgt gaaaaccgtg
     4861 cctgagatca tcaccggcga catggaatct gccatggccg tggacctgaa tccttgggtc
     4921 gagtatgagt tcagagtggt ggccacaaat cccatcggca ccggcgatcc tagcacaccc
     4981 agcagaatga tccggaccaa tgaggccgtg cctaagacag cccctaccaa cgtgtccggc
     5041 agatctggca gaaggcacga gctggttatt gcctgggagc ccgtgtccga ggaattccag
     5101 aatggcgaag gctttggcta catcgtggcc ttcagaccca atggcaccag aggctggaaa
     5161 gaaaagatgg tcaccagctc cgaggccagc aagttcatct accgggatga gagcgtgcca
     5221 ccactgacac ccttcgaagt gaaagtgggc gtgtacaaca acaaaggcga cggccctttc
     5281 agccagatcg tggtcatttg ttctgccgag ggcgagcctt ctgctgcccc aacagatgtg
     5341 aaagccacct ccgtgtctgt gtccgagatc ctggtggcct ggaagcacat caaagagtcc
     5401 ctgggcagac ctcagggctt cgaagtcggc tactggaagg acatggaaca agaggacacc
     5461 gccgagacag tgaaaacaag aggcaacgag agcttcgtga tcctgaccgg actggaaggc
     5521 aacacactgt accactttac cgtgcgggcc tacaacggcg ctggatatgg acctcctagc
     5581 tctgaggtgt ccgccaccac caagaagtct ccaccatctc aggctccctc caatctcaga
     5641 tgggagcagc agggatctca ggtgtccctc ggatgggagc ctgtgatccc tctggccaat
     5701 gagtctgagg tcgtgggcta caaggtgttc taccggcaag agggccacag caacagccaa
     5761 gtgatcgaga cacagaaact gcaggccgtg gtgccacttc ctgatgccgg ggtgtacatc
     5821 atcgaagtcc gggcctattc tgaaggcgga gatggcacag ccagcagcca gattagagtg
     5881 ccatcttaca gcggcggcaa aggcgcgccg gaagatcaag ttgatccaag attgattgat
     5941 ggcaagggcg gatctctgga ggtgctgttc cagggaccag gcggaagcgg caatcccggc
     6001 ggcgatctgg ccccccagat gctgcgcgag ctgcaggaga ccaatgccgc cctgcaggac
     6061 gtgcgcgagc tgctgcgcca ccgcgtgaag gagatcacct tcctgaagaa caccgtgatg
     6121 gagtgcgacg cctgcggtat gcagccctct agaggctccg gctccatcat cccagttgag
     6181 gaggagaacc cggacttctg gaaccgcgag gcagccgagg ccctgggtgc cgccaagaag
     6241 ctgcagcctg cacagacagc cgccaagaac ctcatcatct tcctgggcga tgggatgggg
     6301 gtgtctacgg tgacagctgc caggatccta aaagggcaga agaaggacaa actggggcct
     6361 gagatacccc tggccatgga ccgcttccca tatgtggctc tgtccaagac atacaatgta
     6421 gacaaacatg tgccagacag tggagccaca gccacggcct acctgtgcgg ggtcaagggc
     6481 aacttccaga ccattggctt gagtgcagcc gcccgcttta accagtgcaa cacgacacgc
     6541 ggcaacgagg tcatctccgt gatgaatcgg gccaagaaag cagggaagtc agtgggagtg
     6601 gtaaccacca cacgagtgca gcacgcctcg ccagccggca cctacgccca cacggtgaac
     6661 cgcaactggt actcggacgc cgacgtgcct gcctcggccc gccaggaggg gtgccaggac
     6721 atcgctacgc agctcatctc caacatggac attgacgtga tcctaggtgg aggccgaaag
     6781 tacatgtttc gcatgggaac cccagaccct gagtacccag atgactacag ccaaggtggg
     6841 accaggctgg acgggaagaa tctggtgcag gaatggctgg cgaagcgcca gggtgcccgg
     6901 tatgtgtgga accgcactga gctcatgcag gcttccctgg acccgtctgt gacccatctc
     6961 atgggtctct ttgagcctgg agacatgaaa tacgagatcc accgagactc cacactggac
     7021 ccctccctga tggagatgac agaggctgcc ctgcgcctgc tgagcaggaa cccccgcggc
     7081 ttcttcctct tcgtggaggg tggtcgcatc gaccatggtc atcatgaaag cagggcttac
     7141 cgggcactga ctgagacgat catgttcgac gacgccattg agagggcggg ccagctcacc
     7201 agcgaggagg acacgctgag cctcgtcact gccgaccact cccacgtctt ctccttcgga
     7261 ggctaccccc tgcgagggag ctccatcttc gggctggccc ctggcaaggc ccgggacagg
     7321 aaggcctaca cggtcctcct atacggaaac ggtccaggct atgtgctcaa ggacggcgcc
     7381 cggccggatg ttaccgagag cgagagcggg agccccgagt atcggcagca gtcagcagtg
     7441 cccctggacg aagagaccca cgcaggcgag gacgtggcgg tgttcgcgcg cggcccgcag
     7501 gcgcacctgg ttcacggcgt gcaggagcag accttcatag cgcacgtcat ggccttcgcc
     7561 gcctgcctgg agccctacac cgcctgcgac ctggcgcccc ccgccggcac caccgacgcc
     7621 gcgcacccgg gtggcggagg actgaacgac atcttcgagg ctcagaaaat cgaatggcac
     7681 gaacatcacc atcaccatca ccatcaccat taaggccggc ctgtagtcac tggcacaacg
     7741 cgttacccgg taagccaatc gggtatacac ggtcgtcata ctgcagacag ggttcttcta
     7801 ctttgcaaga tagtcttgag tagtaaaata aatagataga gaaaatcagc ctcgactgtg
     7861 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     7921 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcaca acactcaacc
     7981 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     8041 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     8101 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8161 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8221 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     8281 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8341 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     8401 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     8461 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     8521 aggaactaaa ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     8581 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     8641 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     8701 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     8761 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     8821 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     8881 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     8941 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     9001 cccgccttcc tggagacatc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     9061 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     9121 cccggtgcct gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg
     9181 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     9241 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     9301 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9361 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     9421 gcgtaatcat ggtcattacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9481 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9541 cttaccatct ggccccagcg ctgcgatgat accgcgagaa ccacgctcac cggctccgga
     9601 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9661 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9721 taatagtttg cgcaacgttg ttgccatcgc tacaggcatc gtggtgtcac gctcgtcgtt
     9781 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9841 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9901 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9961 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
    10021 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
    10081 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
    10141 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
    10201 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
    10261 gggaataagg gcgacacgga aatgttgaat actcatattc ttcctt
//
